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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Fish & shellfish immunology
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41380863/ |
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Table of Contents:
- Bacterial infection triggers CD3ε T cell expansion and interaction with macrophages in grass carp (Ctenopharyngodon idella). Gao, Wa Cen, Jing Zhu, Liwen Yan, Hui Ji, Yueshuang Xu, Jing Wang, Bangjie Zhang, Yanwei Zou, Jun Wang, Junya Animals Carps Aeromonas hydrophila Fish Diseases Gram-Negative Bacterial Infections Macrophages T-Lymphocytes Fish Proteins CD3 Complex While T cells are well established as key players in mammalian adaptive immunity, their functional roles in teleosts remain poorly understood. Here, a monoclonal antibody against grass carp (Ctenopharyngodon idella) CD3ε was generated, and characterized by Western blotting, flow cytometry, and immunofluorescence. In healthy fish, CD3ε T cells accounted for 5.1 %, 4.0 %, and 2.6 % of splenic, head kidney, and peripheral blood lymphocytes, respectively. Upon Aeromonas hydrophila infection, CD3ε T cell populations elevated significantly in immune tissues such as spleen, head kidney, and posterior intestine, accompanied by upregulated transcription of T cell markers (Cd3ε, Tcr-β, and Lck). Strikingly, immunofluorescence analysis revealed that CD3ε T cells and MCSFR macrophages were located in the same region in the spleen tissue of A. hydrophila-infected fish, suggesting functional crosstalk between T cells and macrophages. Our findings provide the first evidence of T cell-macrophage interaction in teleost antibacterial defense, offering insights into the adaptive immune responses in fish.