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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
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Current microbiology
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41385108/ |
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| author | Xiao, Jun Yuan, Xin-Ting Chen, Le-Bin OuYang, Yu-Ting Huang, Xin Tan, Jia-Hao Liu, Jun Wang, Shuang Jiao, Jian-Yu Li, Wen-Jun |
| author_facet | Xiao, Jun Yuan, Xin-Ting Chen, Le-Bin OuYang, Yu-Ting Huang, Xin Tan, Jia-Hao Liu, Jun Wang, Shuang Jiao, Jian-Yu Li, Wen-Jun Xiao, Jun Yuan, Xin-Ting Chen, Le-Bin OuYang, Yu-Ting Huang, Xin Tan, Jia-Hao Liu, Jun Wang, Shuang Jiao, Jian-Yu Li, Wen-Jun |
| collection | PubMed - marine biology |
| contents | Brevibacillus nigriterrae sp. nov., a Novel Denitrification Bacterium Isolated from Black Soil. Xiao, Jun Yuan, Xin-Ting Chen, Le-Bin OuYang, Yu-Ting Huang, Xin Tan, Jia-Hao Liu, Jun Wang, Shuang Jiao, Jian-Yu Li, Wen-Jun Soil Microbiology Phylogeny RNA, Ribosomal, 16S Brevibacillus Fatty Acids DNA, Bacterial Base Composition China Bacterial Typing Techniques Denitrification Nucleic Acid Hybridization Phospholipids Vitamin K 2 Sequence Analysis, DNA A Gram-stain-positive, rod-shaped, endospore-forming, immotile and aerobic bacterial strain, designated as SYSU BS000544, was isolated from a black soil sample in Harbin, Heilongjiang province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU BS000544 belongs to the genus Brevibacillus, and showed the highest sequence similarity to Brevibacillus ginsengisoli KCTC 13938 (98.09%). Strain SYSU BS000544 was found to grow at 25-37 °C (optimum, 30 °C) and pH 6.0-7.0 (optimum, pH 6.5) and in the presence of 0% (w/v) NaCl. Polar lipids comprised of phosphophatidylinositol, phosphatidylethanolamine, one unidentified aminophospholipid, and two unidentified polar lipids. The major cellular fatty acids (> 5%) were iso-C ω9c and/or 10-methyl C, iso-C, iso-C, iso-C and anteiso-C. The predominant respiratory quinone was MK-7. The genomic G + C content was 48.84% based on the whole genome analysis. The average nucleotide identity (≤ 78.88%) and digital DNA-DNA hybridization (≤ 15.7%) values between strain SYSU BS000544 and other members of the genus Brevibacillus were all lower than the threshold values recommended for distinguishing novel species. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain SYSU BS000544 represents a novel species of the genus Brevibacillus, for which the name Brevibacillus nigriterrae sp. nov. is proposed. The type strain is SYSU BS000544 (= MCCC 1K09332 = KCTC 43752). |
| format | Artículo científico |
| id | pubmed_41385108 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Current microbiology |
| record_format | pubmed |
| spellingShingle | Brevibacillus nigriterrae sp. nov., a Novel Denitrification Bacterium Isolated from Black Soil. Xiao, Jun Yuan, Xin-Ting Chen, Le-Bin OuYang, Yu-Ting Huang, Xin Tan, Jia-Hao Liu, Jun Wang, Shuang Jiao, Jian-Yu Li, Wen-Jun Soil Microbiology Phylogeny RNA, Ribosomal, 16S Brevibacillus Fatty Acids DNA, Bacterial Base Composition China Bacterial Typing Techniques Denitrification Nucleic Acid Hybridization Phospholipids Vitamin K 2 Sequence Analysis, DNA Brevibacillus nigriterrae sp. nov., a Novel Denitrification Bacterium Isolated from Black Soil. Xiao, Jun Yuan, Xin-Ting Chen, Le-Bin OuYang, Yu-Ting Huang, Xin Tan, Jia-Hao Liu, Jun Wang, Shuang Jiao, Jian-Yu Li, Wen-Jun Soil Microbiology Phylogeny RNA, Ribosomal, 16S Brevibacillus Fatty Acids DNA, Bacterial Base Composition China Bacterial Typing Techniques Denitrification Nucleic Acid Hybridization Phospholipids Vitamin K 2 Sequence Analysis, DNA A Gram-stain-positive, rod-shaped, endospore-forming, immotile and aerobic bacterial strain, designated as SYSU BS000544, was isolated from a black soil sample in Harbin, Heilongjiang province, China. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SYSU BS000544 belongs to the genus Brevibacillus, and showed the highest sequence similarity to Brevibacillus ginsengisoli KCTC 13938 (98.09%). Strain SYSU BS000544 was found to grow at 25-37 °C (optimum, 30 °C) and pH 6.0-7.0 (optimum, pH 6.5) and in the presence of 0% (w/v) NaCl. Polar lipids comprised of phosphophatidylinositol, phosphatidylethanolamine, one unidentified aminophospholipid, and two unidentified polar lipids. The major cellular fatty acids (> 5%) were iso-C ω9c and/or 10-methyl C, iso-C, iso-C, iso-C and anteiso-C. The predominant respiratory quinone was MK-7. The genomic G + C content was 48.84% based on the whole genome analysis. The average nucleotide identity (≤ 78.88%) and digital DNA-DNA hybridization (≤ 15.7%) values between strain SYSU BS000544 and other members of the genus Brevibacillus were all lower than the threshold values recommended for distinguishing novel species. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain SYSU BS000544 represents a novel species of the genus Brevibacillus, for which the name Brevibacillus nigriterrae sp. nov. is proposed. The type strain is SYSU BS000544 (= MCCC 1K09332 = KCTC 43752). |
| title | Brevibacillus nigriterrae sp. nov., a Novel Denitrification Bacterium Isolated from Black Soil. |
| topic | Soil Microbiology Phylogeny RNA, Ribosomal, 16S Brevibacillus Fatty Acids DNA, Bacterial Base Composition China Bacterial Typing Techniques Denitrification Nucleic Acid Hybridization Phospholipids Vitamin K 2 Sequence Analysis, DNA |
| url | https://pubmed.ncbi.nlm.nih.gov/41385108/ |