Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Bioresource technology
2026
|
| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41421672/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266108343025665 |
|---|---|
| author | Liang, Yajing Liu, Weihe Cheng, Chen Liu, Xiang Liu, Zhongqi Wu, Huaizhi Xu, Huifang Lu, Xuefeng |
| author_facet | Liang, Yajing Liu, Weihe Cheng, Chen Liu, Xiang Liu, Zhongqi Wu, Huaizhi Xu, Huifang Lu, Xuefeng Liang, Yajing Liu, Weihe Cheng, Chen Liu, Xiang Liu, Zhongqi Wu, Huaizhi Xu, Huifang Lu, Xuefeng |
| collection | PubMed - marine biology |
| contents | High-Yield production and Kilogram-Scale preparation of 2-O-α-d-Glyceroglycoside through Whole-Cell catalysis using a novel sucrose phosphorylase. Liang, Yajing Liu, Weihe Cheng, Chen Liu, Xiang Liu, Zhongqi Wu, Huaizhi Xu, Huifang Lu, Xuefeng Glucosyltransferases Streptococcus mutans Biocatalysis 2-O-α-d-glyceroglycoside (2-αGG) is a versatile compound with significant potential for applications in cosmetic, pharmaceutical, and food industries. Achieving regio- and stereo-selective biosynthesis with high productivity and recovery continues to be a central challenge in biocatalytic manufacturing. Through bioinformatic exploration and enzymatic characterization, we identified a novel sucrose phosphorylase (SmSP2) from Streptococcus mutans that exhibits the highest transglycosylation activity at the glycerol 2-OH position while demonstrating low hydrolytic activity. We then developed a whole-cell biocatalytic system operating in an unbuffered, salt-free aqueous medium. Using sucrose fed-batch operation, this system achieved unprecedented αGG production of 444 g/L via one-step SPase catalysis. Furthermore, a coupled microfiltration-simulated moving bed (MF-SMB) purification process was developed to enable efficient and environmentally friendly recovery, yielding αGG with a purity of 94.3 % and a product recovery rate exceeding 91.3 %. Pilot-scale (100 L) production successfully generated 42 kg of the αGG product. This work establishes an integrated green manufacturing platform that combines enzymatic precision with industrial scalability for efficient 2-αGG production. |
| format | Artículo científico |
| id | pubmed_41421672 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Bioresource technology |
| record_format | pubmed |
| spellingShingle | High-Yield production and Kilogram-Scale preparation of 2-O-α-d-Glyceroglycoside through Whole-Cell catalysis using a novel sucrose phosphorylase. Liang, Yajing Liu, Weihe Cheng, Chen Liu, Xiang Liu, Zhongqi Wu, Huaizhi Xu, Huifang Lu, Xuefeng Glucosyltransferases Streptococcus mutans Biocatalysis High-Yield production and Kilogram-Scale preparation of 2-O-α-d-Glyceroglycoside through Whole-Cell catalysis using a novel sucrose phosphorylase. Liang, Yajing Liu, Weihe Cheng, Chen Liu, Xiang Liu, Zhongqi Wu, Huaizhi Xu, Huifang Lu, Xuefeng Glucosyltransferases Streptococcus mutans Biocatalysis 2-O-α-d-glyceroglycoside (2-αGG) is a versatile compound with significant potential for applications in cosmetic, pharmaceutical, and food industries. Achieving regio- and stereo-selective biosynthesis with high productivity and recovery continues to be a central challenge in biocatalytic manufacturing. Through bioinformatic exploration and enzymatic characterization, we identified a novel sucrose phosphorylase (SmSP2) from Streptococcus mutans that exhibits the highest transglycosylation activity at the glycerol 2-OH position while demonstrating low hydrolytic activity. We then developed a whole-cell biocatalytic system operating in an unbuffered, salt-free aqueous medium. Using sucrose fed-batch operation, this system achieved unprecedented αGG production of 444 g/L via one-step SPase catalysis. Furthermore, a coupled microfiltration-simulated moving bed (MF-SMB) purification process was developed to enable efficient and environmentally friendly recovery, yielding αGG with a purity of 94.3 % and a product recovery rate exceeding 91.3 %. Pilot-scale (100 L) production successfully generated 42 kg of the αGG product. This work establishes an integrated green manufacturing platform that combines enzymatic precision with industrial scalability for efficient 2-αGG production. |
| title | High-Yield production and Kilogram-Scale preparation of 2-O-α-d-Glyceroglycoside through Whole-Cell catalysis using a novel sucrose phosphorylase. |
| topic | Glucosyltransferases Streptococcus mutans Biocatalysis |
| url | https://pubmed.ncbi.nlm.nih.gov/41421672/ |