Evolutionary dynamics of FoxQ2 transcription factors across metazoans reveals three ancient paralogs.
Fuente:
PubMed
Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Communications biology
2025
|
| Materias: | |
| Acceso en línea: | |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| _version_ | 1868266108337782785 |
|---|---|
| author | Gattoni, Giacomo Lin, Che-Yi York, Joshua R Shew, Colin Keitley, Daniel LaBonne, Carole Yu, Jr-Kai Gillis, J Andrew Benito-Gutiérrez, Elia |
| author_facet | Gattoni, Giacomo Lin, Che-Yi York, Joshua R Shew, Colin Keitley, Daniel LaBonne, Carole Yu, Jr-Kai Gillis, J Andrew Benito-Gutiérrez, Elia Gattoni, Giacomo Lin, Che-Yi York, Joshua R Shew, Colin Keitley, Daniel LaBonne, Carole Yu, Jr-Kai Gillis, J Andrew Benito-Gutiérrez, Elia |
| collection | PubMed - marine biology |
| contents | Evolutionary dynamics of FoxQ2 transcription factors across metazoans reveals three ancient paralogs. Gattoni, Giacomo Lin, Che-Yi York, Joshua R Shew, Colin Keitley, Daniel LaBonne, Carole Yu, Jr-Kai Gillis, J Andrew Benito-Gutiérrez, Elia Animals Forkhead Transcription Factors Evolution, Molecular Phylogeny Synteny FoxQ2 is a highly conserved Forkhead-box transcription factor expressed anteriorly in cnidarians and bilaterians, yet its evolution is marked by rapid divergence and lineage-specific duplications or losses. Moreover, its presence and localization in vertebrate groups remains unclear. To reconcile these conflicting reports of conservation and divergence, we combine phylogenetic and synteny analyses of FoxQ2 sequences from 21 animal phyla. We uncover three ancient FoxQ2 paralogs in bilaterians-FoxQ2I, FoxQ2II, and FoxQ2III. All three were present in the chordate ancestor, and two are retained in vertebrates, indicating a richer FoxQ2 repertoire in vertebrates than previously recognized. To assess FoxQ2 expression, we analyzed mollusk, acoel, amphioxus, and zebrafish single-cell transcriptomic datasets, and conducted fluorescent in situ hybridization in amphioxus, lamprey, skate, zebrafish, and chicken. FoxQ2I and FoxQ2II show conserved anterior expression, while FoxQ2III is expressed in the gut endoderm in chordates, including amphioxus, lamprey, and skate. We also predict conserved transcription factor binding sites across amphioxus genera, revealing stage- and cell-type-specific regulatory interactions for FoxQ2I in deuterostomes. Overall, this work clarifies FoxQ2's evolutionary history, identifies the endodermally expressed paralog FoxQ2III, and proposes that early duplication of FoxQ2I/II enabled subfunctionalization, driving the fast evolutionary rate of FoxQ2 sequences observed in bilaterians. |
| format | Artículo científico |
| id | pubmed_41423499 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Communications biology |
| record_format | pubmed |
| spellingShingle | Evolutionary dynamics of FoxQ2 transcription factors across metazoans reveals three ancient paralogs. Gattoni, Giacomo Lin, Che-Yi York, Joshua R Shew, Colin Keitley, Daniel LaBonne, Carole Yu, Jr-Kai Gillis, J Andrew Benito-Gutiérrez, Elia Animals Forkhead Transcription Factors Evolution, Molecular Phylogeny Synteny Evolutionary dynamics of FoxQ2 transcription factors across metazoans reveals three ancient paralogs. Gattoni, Giacomo Lin, Che-Yi York, Joshua R Shew, Colin Keitley, Daniel LaBonne, Carole Yu, Jr-Kai Gillis, J Andrew Benito-Gutiérrez, Elia Animals Forkhead Transcription Factors Evolution, Molecular Phylogeny Synteny FoxQ2 is a highly conserved Forkhead-box transcription factor expressed anteriorly in cnidarians and bilaterians, yet its evolution is marked by rapid divergence and lineage-specific duplications or losses. Moreover, its presence and localization in vertebrate groups remains unclear. To reconcile these conflicting reports of conservation and divergence, we combine phylogenetic and synteny analyses of FoxQ2 sequences from 21 animal phyla. We uncover three ancient FoxQ2 paralogs in bilaterians-FoxQ2I, FoxQ2II, and FoxQ2III. All three were present in the chordate ancestor, and two are retained in vertebrates, indicating a richer FoxQ2 repertoire in vertebrates than previously recognized. To assess FoxQ2 expression, we analyzed mollusk, acoel, amphioxus, and zebrafish single-cell transcriptomic datasets, and conducted fluorescent in situ hybridization in amphioxus, lamprey, skate, zebrafish, and chicken. FoxQ2I and FoxQ2II show conserved anterior expression, while FoxQ2III is expressed in the gut endoderm in chordates, including amphioxus, lamprey, and skate. We also predict conserved transcription factor binding sites across amphioxus genera, revealing stage- and cell-type-specific regulatory interactions for FoxQ2I in deuterostomes. Overall, this work clarifies FoxQ2's evolutionary history, identifies the endodermally expressed paralog FoxQ2III, and proposes that early duplication of FoxQ2I/II enabled subfunctionalization, driving the fast evolutionary rate of FoxQ2 sequences observed in bilaterians. |
| title | Evolutionary dynamics of FoxQ2 transcription factors across metazoans reveals three ancient paralogs. |
| topic | Animals Forkhead Transcription Factors Evolution, Molecular Phylogeny Synteny |
| url | https://pubmed.ncbi.nlm.nih.gov/41423499/ |