An integrated immunoinformatic approach to design a novel multiepitope chimeric vaccine against as a causal agent of bloodstream infections.
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| Natura: | Artículo científico |
| Lingua: | en |
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Frontiers in immunology
2025
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| _version_ | 1868266108335685632 |
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| author | Yu, Rongrong Hasan, Ahmad Ibrahim, Muhammad Alonazi, Wadi B Bin, Li |
| author_facet | Yu, Rongrong Hasan, Ahmad Ibrahim, Muhammad Alonazi, Wadi B Bin, Li Yu, Rongrong Hasan, Ahmad Ibrahim, Muhammad Alonazi, Wadi B Bin, Li |
| collection | PubMed - marine biology |
| contents | An integrated immunoinformatic approach to design a novel multiepitope chimeric vaccine against as a causal agent of bloodstream infections. Yu, Rongrong Hasan, Ahmad Ibrahim, Muhammad Alonazi, Wadi B Bin, Li Bacterial Vaccines Mycoplasma Infections Humans Mycoplasma Computational Biology Epitopes, B-Lymphocyte Animals Epitopes Proteomics Vaccine Development Sepsis is increasingly recognized as an emerging human pathogen, despite its primary association with marine mammals. It has recently been identified as a causative agent of bloodstream infections and sepsis, a major cause of mortality among hospitalized patients. To date, no approved vaccine is available against , underscoring the urgent need for preventive strategies. The current study was aimed at employing immunoinformatic approaches to design a vaccine based on multiple epitopes derived from the six core proteomic datasets of representative strains. By subtractive genomics, we retrieved 3,576 nonredundant proteins from proteomes following only one putative immunoglobulin-blocking virulence outer membrane protein conserved in six strains. The epitopes derived from the putative immunoglobulin-blocking virulence protein exhibited promising features such as strong binding affinity, lack of allergenicity, nontoxic properties, high antigenicity scores, and excellent solubility. Moreover, these epitopes include nine linear B cell epitopes, eight MHC class I epitopes, and five MHC class II epitopes. In addition, adjuvants and linker molecules were successfully merged into a chimeric vaccine with significant immunogenicity and stimulation of both adaptive and innate immune responses. The promising potential of the selected vaccine candidates was further validated through their favorable physico-chemical characteristics, strong interaction with TLR-4, and stable performance in molecular dynamics simulations. These results suggest that the putative immunoglobulin-blocking outer membrane virulence protein could effectively participate in activating the primary innate immune response, thereby serving as a strong foundation for subsequent adaptive immune activation. The proposed vaccine provides substantial basis for developing effective preventive and therapeutic measures against the zoonotic , whose association with sepsis, soft tissue, and respiratory infections, particularly in immunocompromised individuals emphasizes the crucial need for vaccine development. |
| format | Artículo científico |
| id | pubmed_41425596 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Frontiers in immunology |
| record_format | pubmed |
| spellingShingle | An integrated immunoinformatic approach to design a novel multiepitope chimeric vaccine against as a causal agent of bloodstream infections. Yu, Rongrong Hasan, Ahmad Ibrahim, Muhammad Alonazi, Wadi B Bin, Li Bacterial Vaccines Mycoplasma Infections Humans Mycoplasma Computational Biology Epitopes, B-Lymphocyte Animals Epitopes Proteomics Vaccine Development Sepsis An integrated immunoinformatic approach to design a novel multiepitope chimeric vaccine against as a causal agent of bloodstream infections. Yu, Rongrong Hasan, Ahmad Ibrahim, Muhammad Alonazi, Wadi B Bin, Li Bacterial Vaccines Mycoplasma Infections Humans Mycoplasma Computational Biology Epitopes, B-Lymphocyte Animals Epitopes Proteomics Vaccine Development Sepsis is increasingly recognized as an emerging human pathogen, despite its primary association with marine mammals. It has recently been identified as a causative agent of bloodstream infections and sepsis, a major cause of mortality among hospitalized patients. To date, no approved vaccine is available against , underscoring the urgent need for preventive strategies. The current study was aimed at employing immunoinformatic approaches to design a vaccine based on multiple epitopes derived from the six core proteomic datasets of representative strains. By subtractive genomics, we retrieved 3,576 nonredundant proteins from proteomes following only one putative immunoglobulin-blocking virulence outer membrane protein conserved in six strains. The epitopes derived from the putative immunoglobulin-blocking virulence protein exhibited promising features such as strong binding affinity, lack of allergenicity, nontoxic properties, high antigenicity scores, and excellent solubility. Moreover, these epitopes include nine linear B cell epitopes, eight MHC class I epitopes, and five MHC class II epitopes. In addition, adjuvants and linker molecules were successfully merged into a chimeric vaccine with significant immunogenicity and stimulation of both adaptive and innate immune responses. The promising potential of the selected vaccine candidates was further validated through their favorable physico-chemical characteristics, strong interaction with TLR-4, and stable performance in molecular dynamics simulations. These results suggest that the putative immunoglobulin-blocking outer membrane virulence protein could effectively participate in activating the primary innate immune response, thereby serving as a strong foundation for subsequent adaptive immune activation. The proposed vaccine provides substantial basis for developing effective preventive and therapeutic measures against the zoonotic , whose association with sepsis, soft tissue, and respiratory infections, particularly in immunocompromised individuals emphasizes the crucial need for vaccine development. |
| title | An integrated immunoinformatic approach to design a novel multiepitope chimeric vaccine against as a causal agent of bloodstream infections. |
| topic | Bacterial Vaccines Mycoplasma Infections Humans Mycoplasma Computational Biology Epitopes, B-Lymphocyte Animals Epitopes Proteomics Vaccine Development Sepsis |
| url | https://pubmed.ncbi.nlm.nih.gov/41425596/ |