Identification of molecular nociceptors in Octopus vulgaris through functional characterisation in Caenorhabditis elegans.
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| Main Authors: | , , , , , |
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| Format: | Artículo científico |
| Language: | en |
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Biology open
2026
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| _version_ | 1868266108332539905 |
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| author | Pieroni, Eleonora Maria O'Connor, Vincent Holden-Dye, Lindy Imperadore, Pamela Fiorito, Graziano Dillon, James |
| author_facet | Pieroni, Eleonora Maria O'Connor, Vincent Holden-Dye, Lindy Imperadore, Pamela Fiorito, Graziano Dillon, James Pieroni, Eleonora Maria O'Connor, Vincent Holden-Dye, Lindy Imperadore, Pamela Fiorito, Graziano Dillon, James |
| collection | PubMed - marine biology |
| contents | Identification of molecular nociceptors in Octopus vulgaris through functional characterisation in Caenorhabditis elegans. Pieroni, Eleonora Maria O'Connor, Vincent Holden-Dye, Lindy Imperadore, Pamela Fiorito, Graziano Dillon, James Animals Caenorhabditis elegans Nociceptors Octopodiformes Computational Biology Transcriptome Nociception Gene Expression Profiling Nociception, a phenomenon crucial for animal survival, deploys evolutionarily conserved molecular mechanisms. Among invertebrate species, cephalopods are of particular interest as they possess a well-developed brain speculated to be able to encode pain-like states. This has led to their inclusion in the Directive 2010/63 EU for welfare protection. However, the molecular mechanisms of nociception in cephalopods are still poorly characterised and it is important to address this knowledge gap to better understand cephalopods' capacity to express pain states. Here we describe a bioinformatic strategy utilising conserved nociceptive genes, to identify the orthologous candidates in the Octopus vulgaris transcriptome. We identified 51 genes we predict to function in nociception. These add to the mechanosensory TRPN and the unique chemotactile receptors recently identified in octopus suckers, thus expanding the set of genes that merit further functional characterisation in cephalopods. We therefore selected 38 orthologues in Caenorhabditis elegans, a tractable experimental platform and tested loss of function mutant strains of distinct functional gene classes (e.g. osm-9, egl-3, frpr-3) in a low pH avoidance paradigm. This identified 19 nociceptive-related genes to be prioritised for further functional characterisation in O. vulgaris. |
| format | Artículo científico |
| id | pubmed_41431869 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Biology open |
| record_format | pubmed |
| spellingShingle | Identification of molecular nociceptors in Octopus vulgaris through functional characterisation in Caenorhabditis elegans. Pieroni, Eleonora Maria O'Connor, Vincent Holden-Dye, Lindy Imperadore, Pamela Fiorito, Graziano Dillon, James Animals Caenorhabditis elegans Nociceptors Octopodiformes Computational Biology Transcriptome Nociception Gene Expression Profiling Identification of molecular nociceptors in Octopus vulgaris through functional characterisation in Caenorhabditis elegans. Pieroni, Eleonora Maria O'Connor, Vincent Holden-Dye, Lindy Imperadore, Pamela Fiorito, Graziano Dillon, James Animals Caenorhabditis elegans Nociceptors Octopodiformes Computational Biology Transcriptome Nociception Gene Expression Profiling Nociception, a phenomenon crucial for animal survival, deploys evolutionarily conserved molecular mechanisms. Among invertebrate species, cephalopods are of particular interest as they possess a well-developed brain speculated to be able to encode pain-like states. This has led to their inclusion in the Directive 2010/63 EU for welfare protection. However, the molecular mechanisms of nociception in cephalopods are still poorly characterised and it is important to address this knowledge gap to better understand cephalopods' capacity to express pain states. Here we describe a bioinformatic strategy utilising conserved nociceptive genes, to identify the orthologous candidates in the Octopus vulgaris transcriptome. We identified 51 genes we predict to function in nociception. These add to the mechanosensory TRPN and the unique chemotactile receptors recently identified in octopus suckers, thus expanding the set of genes that merit further functional characterisation in cephalopods. We therefore selected 38 orthologues in Caenorhabditis elegans, a tractable experimental platform and tested loss of function mutant strains of distinct functional gene classes (e.g. osm-9, egl-3, frpr-3) in a low pH avoidance paradigm. This identified 19 nociceptive-related genes to be prioritised for further functional characterisation in O. vulgaris. |
| title | Identification of molecular nociceptors in Octopus vulgaris through functional characterisation in Caenorhabditis elegans. |
| topic | Animals Caenorhabditis elegans Nociceptors Octopodiformes Computational Biology Transcriptome Nociception Gene Expression Profiling |
| url | https://pubmed.ncbi.nlm.nih.gov/41431869/ |