A comparative review of vector insertion techniques in Saccharomyces cerevisiae.
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| Format: | Artículo científico |
| Language: | en |
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Journal of microbiological methods
2026
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| _version_ | 1868266108328345601 |
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| author | Gopalakrishnan, Ritika Kannan, Kamala Gunasekaran, Ragul Ramachandran, Priya Ganapathy, Dhanraj Pitchiah, Sivaperumal |
| author_facet | Gopalakrishnan, Ritika Kannan, Kamala Gunasekaran, Ragul Ramachandran, Priya Ganapathy, Dhanraj Pitchiah, Sivaperumal Gopalakrishnan, Ritika Kannan, Kamala Gunasekaran, Ragul Ramachandran, Priya Ganapathy, Dhanraj Pitchiah, Sivaperumal |
| collection | PubMed - marine biology |
| contents | A comparative review of vector insertion techniques in Saccharomyces cerevisiae. Gopalakrishnan, Ritika Kannan, Kamala Gunasekaran, Ragul Ramachandran, Priya Ganapathy, Dhanraj Pitchiah, Sivaperumal CRISPR-Cas Systems Gene Editing Genetic Engineering Genetic Vectors Genome, Fungal Homologous Recombination Mutagenesis, Insertional Saccharomyces cerevisiae Synthetic Biology Saccharomyces cerevisiae, a model organism in genetics and molecular biology has been extensively engineered using various vector insertion techniques. This review compares and contrasts three prominent techniques: In vivo homologous recombination (HR), Cre-lox recombination and CRISPR/Cas9. In vivo HR leverages the organism's innate DNA repair machinery for easy vector integration and targeted genome modifications. Cre-lox recombination offers high specificity and efficiency at loxP sites, making it ideal for targeted gene excision or integration. CRISPR/Cas9 has revolutionized genome engineering with its precision and ability to target multiple loci simultaneously. Each technique has its strengths and limitations, including site dependency, off-target effects, and strain-specific variability. This review provides a comprehensive overview of these vector insertion techniques, highlighting their applications, advantages, and limitations in S. cerevisiae genome engineering and synthetic biology. |
| format | Artículo científico |
| id | pubmed_41435996 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Journal of microbiological methods |
| record_format | pubmed |
| spellingShingle | A comparative review of vector insertion techniques in Saccharomyces cerevisiae. Gopalakrishnan, Ritika Kannan, Kamala Gunasekaran, Ragul Ramachandran, Priya Ganapathy, Dhanraj Pitchiah, Sivaperumal CRISPR-Cas Systems Gene Editing Genetic Engineering Genetic Vectors Genome, Fungal Homologous Recombination Mutagenesis, Insertional Saccharomyces cerevisiae Synthetic Biology A comparative review of vector insertion techniques in Saccharomyces cerevisiae. Gopalakrishnan, Ritika Kannan, Kamala Gunasekaran, Ragul Ramachandran, Priya Ganapathy, Dhanraj Pitchiah, Sivaperumal CRISPR-Cas Systems Gene Editing Genetic Engineering Genetic Vectors Genome, Fungal Homologous Recombination Mutagenesis, Insertional Saccharomyces cerevisiae Synthetic Biology Saccharomyces cerevisiae, a model organism in genetics and molecular biology has been extensively engineered using various vector insertion techniques. This review compares and contrasts three prominent techniques: In vivo homologous recombination (HR), Cre-lox recombination and CRISPR/Cas9. In vivo HR leverages the organism's innate DNA repair machinery for easy vector integration and targeted genome modifications. Cre-lox recombination offers high specificity and efficiency at loxP sites, making it ideal for targeted gene excision or integration. CRISPR/Cas9 has revolutionized genome engineering with its precision and ability to target multiple loci simultaneously. Each technique has its strengths and limitations, including site dependency, off-target effects, and strain-specific variability. This review provides a comprehensive overview of these vector insertion techniques, highlighting their applications, advantages, and limitations in S. cerevisiae genome engineering and synthetic biology. |
| title | A comparative review of vector insertion techniques in Saccharomyces cerevisiae. |
| topic | CRISPR-Cas Systems Gene Editing Genetic Engineering Genetic Vectors Genome, Fungal Homologous Recombination Mutagenesis, Insertional Saccharomyces cerevisiae Synthetic Biology |
| url | https://pubmed.ncbi.nlm.nih.gov/41435996/ |