Anti-Cancer Activity of Algal Extract: Hopes and Fears of a Possible Alternative Treatment for Canine Mast Cell Tumor.
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| Format: | Artículo científico |
| Sprache: | en |
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Marine drugs
2025
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| author | Mucignat, Greta Lakhdar, Fatima Maghrebi, Hanane Dejnaka, Ewa Lucatello, Lorena Benhniya, Bouchra Capolongo, Francesca Etahiri, Samira Pauletto, Marianna Pawlak, Aleksandra Giantin, Mery Dacasto, Mauro |
| author_facet | Mucignat, Greta Lakhdar, Fatima Maghrebi, Hanane Dejnaka, Ewa Lucatello, Lorena Benhniya, Bouchra Capolongo, Francesca Etahiri, Samira Pauletto, Marianna Pawlak, Aleksandra Giantin, Mery Dacasto, Mauro Mucignat, Greta Lakhdar, Fatima Maghrebi, Hanane Dejnaka, Ewa Lucatello, Lorena Benhniya, Bouchra Capolongo, Francesca Etahiri, Samira Pauletto, Marianna Pawlak, Aleksandra Giantin, Mery Dacasto, Mauro |
| collection | PubMed - marine biology |
| contents | Anti-Cancer Activity of Algal Extract: Hopes and Fears of a Possible Alternative Treatment for Canine Mast Cell Tumor. Mucignat, Greta Lakhdar, Fatima Maghrebi, Hanane Dejnaka, Ewa Lucatello, Lorena Benhniya, Bouchra Capolongo, Francesca Etahiri, Samira Pauletto, Marianna Pawlak, Aleksandra Giantin, Mery Dacasto, Mauro Animals Cell Line, Tumor Antineoplastic Agents Dogs Mastocytoma Apoptosis Cell Cycle DNA Damage Within the "One Health, One Medicine" and comparative oncology paradigms, algal extracts have attracted attention, containing natural compounds (NCs) with biological activities, including anti-cancer properties. To characterize the biological effects of a extract (SCE), two canine mastocytoma and two normal cell lines were used. After a preliminary screening of three algal extracts, SCE cytotoxicity was measured using Alamar Blue, Sulforhodamine B, and Neutral Red Uptake assays. After assessing the selectivity versus tumor cells and its chemical characterization, SCE mechanisms of action were investigated using RNA-seq, quantitative PCR, flow cytometry and immunoblotting approaches. SCE showed an IC comprised between 25 and 35 μg/mL in tumor cell lines, but it also affected normal ones (selectivity index < 2.0). RNA-seq and flow cytometry revealed that SCE negatively affected cell cycle and mevalonate pathway in tumor cells. Additional flow cytometry and immunoblotting investigations suggested a concentration- and time-dependent pro-apoptotic effect of SCE and DNA damage events. In conclusion, SCE demonstrated promising anti-cancer activity in mastocytoma cell lines by targeting the mevalonate pathway, arresting the cell cycle, and inducing apoptosis and DNA damage. Furthermore, the results presented here reinforce the idea that NCs may be promising candidates in comparative anti-cancer chemotherapy. |
| format | Artículo científico |
| id | pubmed_41440891 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Marine drugs |
| record_format | pubmed |
| spellingShingle | Anti-Cancer Activity of Algal Extract: Hopes and Fears of a Possible Alternative Treatment for Canine Mast Cell Tumor. Mucignat, Greta Lakhdar, Fatima Maghrebi, Hanane Dejnaka, Ewa Lucatello, Lorena Benhniya, Bouchra Capolongo, Francesca Etahiri, Samira Pauletto, Marianna Pawlak, Aleksandra Giantin, Mery Dacasto, Mauro Animals Cell Line, Tumor Antineoplastic Agents Dogs Mastocytoma Apoptosis Cell Cycle DNA Damage Anti-Cancer Activity of Algal Extract: Hopes and Fears of a Possible Alternative Treatment for Canine Mast Cell Tumor. Mucignat, Greta Lakhdar, Fatima Maghrebi, Hanane Dejnaka, Ewa Lucatello, Lorena Benhniya, Bouchra Capolongo, Francesca Etahiri, Samira Pauletto, Marianna Pawlak, Aleksandra Giantin, Mery Dacasto, Mauro Animals Cell Line, Tumor Antineoplastic Agents Dogs Mastocytoma Apoptosis Cell Cycle DNA Damage Within the "One Health, One Medicine" and comparative oncology paradigms, algal extracts have attracted attention, containing natural compounds (NCs) with biological activities, including anti-cancer properties. To characterize the biological effects of a extract (SCE), two canine mastocytoma and two normal cell lines were used. After a preliminary screening of three algal extracts, SCE cytotoxicity was measured using Alamar Blue, Sulforhodamine B, and Neutral Red Uptake assays. After assessing the selectivity versus tumor cells and its chemical characterization, SCE mechanisms of action were investigated using RNA-seq, quantitative PCR, flow cytometry and immunoblotting approaches. SCE showed an IC comprised between 25 and 35 μg/mL in tumor cell lines, but it also affected normal ones (selectivity index < 2.0). RNA-seq and flow cytometry revealed that SCE negatively affected cell cycle and mevalonate pathway in tumor cells. Additional flow cytometry and immunoblotting investigations suggested a concentration- and time-dependent pro-apoptotic effect of SCE and DNA damage events. In conclusion, SCE demonstrated promising anti-cancer activity in mastocytoma cell lines by targeting the mevalonate pathway, arresting the cell cycle, and inducing apoptosis and DNA damage. Furthermore, the results presented here reinforce the idea that NCs may be promising candidates in comparative anti-cancer chemotherapy. |
| title | Anti-Cancer Activity of Algal Extract: Hopes and Fears of a Possible Alternative Treatment for Canine Mast Cell Tumor. |
| topic | Animals Cell Line, Tumor Antineoplastic Agents Dogs Mastocytoma Apoptosis Cell Cycle DNA Damage |
| url | https://pubmed.ncbi.nlm.nih.gov/41440891/ |