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| Format: | Artículo científico |
| Sprache: | en |
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Metabolites
2025
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/41441029/ |
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| author | Carbonell-Garzón, Estela Ibanco-Cañete, Ricardo Sanchez-Jerez, Pablo Egea, Frutos C Marhuenda |
| author_facet | Carbonell-Garzón, Estela Ibanco-Cañete, Ricardo Sanchez-Jerez, Pablo Egea, Frutos C Marhuenda Carbonell-Garzón, Estela Ibanco-Cañete, Ricardo Sanchez-Jerez, Pablo Egea, Frutos C Marhuenda |
| collection | PubMed - marine biology |
| contents | Osmolytes vs. Anabolic Reserves: Contrasting Gonadal Metabolomes in Two Sympatric Mediterranean Sea Urchins. Carbonell-Garzón, Estela Ibanco-Cañete, Ricardo Sanchez-Jerez, Pablo Egea, Frutos C Marhuenda The Mediterranean sea urchins and co-occur on shallow rocky reefs but display contrasting ecological and physiological traits. We compared their gonadal metabolomes to identify species-specific metabolic strategies. High-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy to intact gonadal tissues, combining multivariate chemometric modelling with targeted integration, boxplot-based univariate analysis and pathway analysis. showed an osmolyte- and redox-oriented phenotype with elevated betaine, taurine, sarcosine, trimethylamine (TMA), trimethylamine N-oxide (TMAO), carnitine, creatine, malonate, methylmalonate, uridine and xanthine. In contrast, exhibited an amino-acid-enriched anabolic profile dominated by lysine, glycine and glutamine, together with higher levels of formaldehyde, methanol and 3-carboxypropyl-trimethylammonium. Pathway analysis indicated that metabolites mapped onto glycine/serine-threonine metabolism and the folate-linked one-carbon pool, whereas metabolites were enriched in glyoxylate/dicarboxylate, nitrogen and amino-acid pathways. These contrasting osmolyte-C versus nitrogen-amino-acid strategies are compatible with species-specific host-microbiota metabolic interactions inferred from published microbiome data. Overall, our results support a framework in which adopts a resilience-oriented osmolyte strategy and an efficiency-oriented anabolic strategy, highlighting HR-MAS NMR metabolomics as a powerful approach to investigate adaptive biochemical diversity in marine invertebrates. |
| format | Artículo científico |
| id | pubmed_41441029 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Metabolites |
| record_format | pubmed |
| spellingShingle | Osmolytes vs. Anabolic Reserves: Contrasting Gonadal Metabolomes in Two Sympatric Mediterranean Sea Urchins. Carbonell-Garzón, Estela Ibanco-Cañete, Ricardo Sanchez-Jerez, Pablo Egea, Frutos C Marhuenda Osmolytes vs. Anabolic Reserves: Contrasting Gonadal Metabolomes in Two Sympatric Mediterranean Sea Urchins. Carbonell-Garzón, Estela Ibanco-Cañete, Ricardo Sanchez-Jerez, Pablo Egea, Frutos C Marhuenda The Mediterranean sea urchins and co-occur on shallow rocky reefs but display contrasting ecological and physiological traits. We compared their gonadal metabolomes to identify species-specific metabolic strategies. High-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy to intact gonadal tissues, combining multivariate chemometric modelling with targeted integration, boxplot-based univariate analysis and pathway analysis. showed an osmolyte- and redox-oriented phenotype with elevated betaine, taurine, sarcosine, trimethylamine (TMA), trimethylamine N-oxide (TMAO), carnitine, creatine, malonate, methylmalonate, uridine and xanthine. In contrast, exhibited an amino-acid-enriched anabolic profile dominated by lysine, glycine and glutamine, together with higher levels of formaldehyde, methanol and 3-carboxypropyl-trimethylammonium. Pathway analysis indicated that metabolites mapped onto glycine/serine-threonine metabolism and the folate-linked one-carbon pool, whereas metabolites were enriched in glyoxylate/dicarboxylate, nitrogen and amino-acid pathways. These contrasting osmolyte-C versus nitrogen-amino-acid strategies are compatible with species-specific host-microbiota metabolic interactions inferred from published microbiome data. Overall, our results support a framework in which adopts a resilience-oriented osmolyte strategy and an efficiency-oriented anabolic strategy, highlighting HR-MAS NMR metabolomics as a powerful approach to investigate adaptive biochemical diversity in marine invertebrates. |
| title | Osmolytes vs. Anabolic Reserves: Contrasting Gonadal Metabolomes in Two Sympatric Mediterranean Sea Urchins. |
| url | https://pubmed.ncbi.nlm.nih.gov/41441029/ |