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Hauptverfasser: Cao, Yun-Chao, Zhou, Yu-Qing, Wu, Jia-Yao, Munang'andu, Hetron Mweemba, Peng, Bo
Format: Artículo científico
Sprache:en
Veröffentlicht: Journal of hazardous materials 2026
Schlagworte:
Online-Zugang:https://pubmed.ncbi.nlm.nih.gov/41455227/
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author Cao, Yun-Chao
Zhou, Yu-Qing
Wu, Jia-Yao
Munang'andu, Hetron Mweemba
Peng, Bo
author_facet Cao, Yun-Chao
Zhou, Yu-Qing
Wu, Jia-Yao
Munang'andu, Hetron Mweemba
Peng, Bo
Cao, Yun-Chao
Zhou, Yu-Qing
Wu, Jia-Yao
Munang'andu, Hetron Mweemba
Peng, Bo
collection PubMed - marine biology
contents Arachidonic acid reverses microplastic-induced macrophage dysfunction in teleost fish. Cao, Yun-Chao Zhou, Yu-Qing Wu, Jia-Yao Munang'andu, Hetron Mweemba Peng, Bo Animals Macrophages Microplastics Arachidonic Acid Cichlids Water Pollutants, Chemical Polystyrenes Phagocytosis Lysosomes Streptococcal Infections Oxidative Stress Cytokines Fish Diseases T-Lymphocytes Microplastic pollution poses a significant threat to aquaculture by compromising fish immunity, particularly macrophage function. This study investigated the impact of polystyrene microplastics (PS) on Nile tilapia (Oreochromis niloticus) macrophages and explored metabolic interventions to reverse PS-induced damage. PS exposure increased tilapia susceptibility to Streptococcus agalactiae infection, reducing fish survival. PS accumulated in head kidney macrophages, impairing phagocytosis, altering cytokine expression, elevating oxidative stress and malondialdehyde levels, and suppressing T-cell proliferation. Transcriptomics revealed PS dysregulated lysosomal pathways, reducing lysosomal membrane permeability and bacterial killing capacity. Metabolomic screening identified arachidonic acid (AA) as the most significantly suppressed metabolite in PS-exposed macrophages. Exogenous AA administration restored macrophage function including phagocytosis, cytokine expression, oxidative stress, enhanced lysosomal integrity, improved bactericidal activity, and increased survival during S. agalactiae challenge in PS-exposed fish. AA also reversed PS-induced transcriptional dysregulation of lysosomal genes. These results demonstrate that AA rectifies PS-induced macrophage dysfunction and lysosomal impairment, supporting its potential as a dietary supplement to mitigate microplastic immunotoxicity in aquaculture.
format Artículo científico
id pubmed_41455227
institution PubMed
language en
publishDate 2026
publisher Journal of hazardous materials
record_format pubmed
spellingShingle Arachidonic acid reverses microplastic-induced macrophage dysfunction in teleost fish.
Cao, Yun-Chao
Zhou, Yu-Qing
Wu, Jia-Yao
Munang'andu, Hetron Mweemba
Peng, Bo
Animals
Macrophages
Microplastics
Arachidonic Acid
Cichlids
Water Pollutants, Chemical
Polystyrenes
Phagocytosis
Lysosomes
Streptococcal Infections
Oxidative Stress
Cytokines
Fish Diseases
T-Lymphocytes
Arachidonic acid reverses microplastic-induced macrophage dysfunction in teleost fish. Cao, Yun-Chao Zhou, Yu-Qing Wu, Jia-Yao Munang'andu, Hetron Mweemba Peng, Bo Animals Macrophages Microplastics Arachidonic Acid Cichlids Water Pollutants, Chemical Polystyrenes Phagocytosis Lysosomes Streptococcal Infections Oxidative Stress Cytokines Fish Diseases T-Lymphocytes Microplastic pollution poses a significant threat to aquaculture by compromising fish immunity, particularly macrophage function. This study investigated the impact of polystyrene microplastics (PS) on Nile tilapia (Oreochromis niloticus) macrophages and explored metabolic interventions to reverse PS-induced damage. PS exposure increased tilapia susceptibility to Streptococcus agalactiae infection, reducing fish survival. PS accumulated in head kidney macrophages, impairing phagocytosis, altering cytokine expression, elevating oxidative stress and malondialdehyde levels, and suppressing T-cell proliferation. Transcriptomics revealed PS dysregulated lysosomal pathways, reducing lysosomal membrane permeability and bacterial killing capacity. Metabolomic screening identified arachidonic acid (AA) as the most significantly suppressed metabolite in PS-exposed macrophages. Exogenous AA administration restored macrophage function including phagocytosis, cytokine expression, oxidative stress, enhanced lysosomal integrity, improved bactericidal activity, and increased survival during S. agalactiae challenge in PS-exposed fish. AA also reversed PS-induced transcriptional dysregulation of lysosomal genes. These results demonstrate that AA rectifies PS-induced macrophage dysfunction and lysosomal impairment, supporting its potential as a dietary supplement to mitigate microplastic immunotoxicity in aquaculture.
title Arachidonic acid reverses microplastic-induced macrophage dysfunction in teleost fish.
topic Animals
Macrophages
Microplastics
Arachidonic Acid
Cichlids
Water Pollutants, Chemical
Polystyrenes
Phagocytosis
Lysosomes
Streptococcal Infections
Oxidative Stress
Cytokines
Fish Diseases
T-Lymphocytes
url https://pubmed.ncbi.nlm.nih.gov/41455227/