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| Autores principales: | , , , , , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Journal of biological engineering
2025
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| Acceso en línea: | https://pubmed.ncbi.nlm.nih.gov/41466312/ |
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| _version_ | 1868266105543327744 |
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| author | Chen, Yu Burke, Adam Chriscoli, Vincent Yang, Mengru Chang, Ping Li, Tianpei Zhang, Buke Goodacre, Royston Liu, Lu-Ning |
| author_facet | Chen, Yu Burke, Adam Chriscoli, Vincent Yang, Mengru Chang, Ping Li, Tianpei Zhang, Buke Goodacre, Royston Liu, Lu-Ning Chen, Yu Burke, Adam Chriscoli, Vincent Yang, Mengru Chang, Ping Li, Tianpei Zhang, Buke Goodacre, Royston Liu, Lu-Ning |
| collection | PubMed - marine biology |
| contents | Reprogramme the E. coli metabolism by engineering a functional carbon-fixation pathway. Chen, Yu Burke, Adam Chriscoli, Vincent Yang, Mengru Chang, Ping Li, Tianpei Zhang, Buke Goodacre, Royston Liu, Lu-Ning BACKGROUND: Rising atmospheric CO₂ levels and their impact on climate change have intensified the need for innovative carbon capture and fixation strategies. The Calvin-Benson-Bassham (CBB) cycle, a central metabolic pathway in all photoautotrophic organisms and many autotrophic bacteria, plays a pivotal role in global carbon assimilation but is limited by the low catalytic efficiency of Rubisco. RESULTS: Here, we engineered a complete, functional CBB cycle in Escherichia coli, by heterologously expressing up to 13 genes encoding phosphoribulokinase, α-carboxysomes, and inorganic carbon pumps. This bioengineering approach allowed E. coli to utilize atmospheric CO2 and led to increased levels of sugars such as ribose (4.94-fold) and xylitol (8.94-fold). Detailed metabolomic profiling of central carbon metabolism using gas chromatography-mass spectrometry (GC-MS) demonstrated that installation of the CBB cycle has a notable impact on the metabolic landscape of E. coli, resulting in substantial alterations in central carbon and amino acid metabolism. These findings deepen our understanding of the natural biological carbon-fixation pathway and its engineering in heterotrophic hosts. Furthermore, this work provides a versatile platform for evaluating and selecting efficient carbon-fixation modules, as well as assessing metabolic bottlenecks in engineered systems. CONCLUSION: These advances offer practical guidance for rational metabolic engineering in diverse organisms for biotechnological applications, including carbon sequestration, sustainable bioproduction, and crop improvement. |
| format | Artículo científico |
| id | pubmed_41466312 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Journal of biological engineering |
| record_format | pubmed |
| spellingShingle | Reprogramme the E. coli metabolism by engineering a functional carbon-fixation pathway. Chen, Yu Burke, Adam Chriscoli, Vincent Yang, Mengru Chang, Ping Li, Tianpei Zhang, Buke Goodacre, Royston Liu, Lu-Ning Reprogramme the E. coli metabolism by engineering a functional carbon-fixation pathway. Chen, Yu Burke, Adam Chriscoli, Vincent Yang, Mengru Chang, Ping Li, Tianpei Zhang, Buke Goodacre, Royston Liu, Lu-Ning BACKGROUND: Rising atmospheric CO₂ levels and their impact on climate change have intensified the need for innovative carbon capture and fixation strategies. The Calvin-Benson-Bassham (CBB) cycle, a central metabolic pathway in all photoautotrophic organisms and many autotrophic bacteria, plays a pivotal role in global carbon assimilation but is limited by the low catalytic efficiency of Rubisco. RESULTS: Here, we engineered a complete, functional CBB cycle in Escherichia coli, by heterologously expressing up to 13 genes encoding phosphoribulokinase, α-carboxysomes, and inorganic carbon pumps. This bioengineering approach allowed E. coli to utilize atmospheric CO2 and led to increased levels of sugars such as ribose (4.94-fold) and xylitol (8.94-fold). Detailed metabolomic profiling of central carbon metabolism using gas chromatography-mass spectrometry (GC-MS) demonstrated that installation of the CBB cycle has a notable impact on the metabolic landscape of E. coli, resulting in substantial alterations in central carbon and amino acid metabolism. These findings deepen our understanding of the natural biological carbon-fixation pathway and its engineering in heterotrophic hosts. Furthermore, this work provides a versatile platform for evaluating and selecting efficient carbon-fixation modules, as well as assessing metabolic bottlenecks in engineered systems. CONCLUSION: These advances offer practical guidance for rational metabolic engineering in diverse organisms for biotechnological applications, including carbon sequestration, sustainable bioproduction, and crop improvement. |
| title | Reprogramme the E. coli metabolism by engineering a functional carbon-fixation pathway. |
| url | https://pubmed.ncbi.nlm.nih.gov/41466312/ |