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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Animals : an open access journal from MDPI
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41514701/ |
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Table of Contents:
- Establishment of a CRISPR/Cas9-Based Genome Editing System in . Yan, Junjun Qian, Siyu Li, Guo Liu, Yujie Zhou, Liqian Ye, Tiantian Liu, Cui Meng, Jilun Jie, Yukun Gu, Zhimin The giant freshwater prawn is a globally farmed decapod species of high economic and nutritional value, but its genetic improvement has been constrained by the lack of an efficient genome editing workflow. Here, we optimized a CRISPR/Cas9 microinjection system for targeted mutagenesis in embryos. A controlled mating-based approach enabled routine collection of one-cell embryos, and developmental staging identified 0.5-2 h post-fertilization as the optimal injection window. Optimization of the needle tip (~1 μm) minimized mechanical damage, lowering early embryo mortality to ~10%. Using this system, the eye-development gene and the sex-related gene were successfully edited. editing produced a 46.9% mutation rate and an average 50% reduction in eye pigment area. editing achieved an 84% mutation rate, while post-injection survival to hatching was approximately 35%. These results establish an efficient and reproducible CRISPR/Cas9 editing system in , providing a foundation for functional genomics and genetic improvement in this commercially important prawn species.