Evaluation of an Infertile, All-Male ZZ Line Exhibiting Female-like Growth in Chinese Tongue Sole (): Growth Performance, Flesh Quality, and Muscle Metabolome.
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| Format: | Artículo científico |
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Biology
2026
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| author | Chen, Zhangfan Wu, Yinqi Ding, Lanqing Li, Pengfei Wang, Mengqi Yan, Xu Cheng, Fangzhou Jiang, He Cui, Zhongkai Chen, Songlin |
| author_facet | Chen, Zhangfan Wu, Yinqi Ding, Lanqing Li, Pengfei Wang, Mengqi Yan, Xu Cheng, Fangzhou Jiang, He Cui, Zhongkai Chen, Songlin Chen, Zhangfan Wu, Yinqi Ding, Lanqing Li, Pengfei Wang, Mengqi Yan, Xu Cheng, Fangzhou Jiang, He Cui, Zhongkai Chen, Songlin |
| collection | PubMed - marine biology |
| contents | Evaluation of an Infertile, All-Male ZZ Line Exhibiting Female-like Growth in Chinese Tongue Sole (): Growth Performance, Flesh Quality, and Muscle Metabolome. Chen, Zhangfan Wu, Yinqi Ding, Lanqing Li, Pengfei Wang, Mengqi Yan, Xu Cheng, Fangzhou Jiang, He Cui, Zhongkai Chen, Songlin Chinese tongue sole () is an important marine aquaculture species that exhibits pronounced female-biased sexual size dimorphism, which limits the economic value of male stocks. The F4 generation of genome-edited ZZ males carried a stable 8 bp deletion in , developed ovarian lamella-like gonads and were completely sterile, as in vitro fertilization with their gonads produced no viable embryos. From 12 to 15 months post-hatch (mph), their growth rate was significantly higher than that of wild-type males and ultimately comparable to that of females. They weighed 3.2-fold heavier and measured 1.38-fold longer than their 15 mph wild-type male counterparts. Muscle nutritional composition and most texture traits of ZZ males did not differ from wild-type females. However, their fat content was significantly lower than that of wild-type males, while their Omega6 content was significantly higher. Metabolomic analysis identified 1262 metabolites and revealed differential enrichment of pathways related to amino acids, energy, and antioxidant and neuromuscular metabolism, without evidence of detrimental shifts. Overall, editing yields a fast-growing, functionally sterile male line whose flesh quality is similar to that of wild-type tongue sole, supporting its potential use in sex-control breeding in Chinese tongue sole and providing a valuable model for studying sexual size dimorphism. |
| format | Artículo científico |
| id | pubmed_41514934 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Biology |
| record_format | pubmed |
| spellingShingle | Evaluation of an Infertile, All-Male ZZ Line Exhibiting Female-like Growth in Chinese Tongue Sole (): Growth Performance, Flesh Quality, and Muscle Metabolome. Chen, Zhangfan Wu, Yinqi Ding, Lanqing Li, Pengfei Wang, Mengqi Yan, Xu Cheng, Fangzhou Jiang, He Cui, Zhongkai Chen, Songlin Evaluation of an Infertile, All-Male ZZ Line Exhibiting Female-like Growth in Chinese Tongue Sole (): Growth Performance, Flesh Quality, and Muscle Metabolome. Chen, Zhangfan Wu, Yinqi Ding, Lanqing Li, Pengfei Wang, Mengqi Yan, Xu Cheng, Fangzhou Jiang, He Cui, Zhongkai Chen, Songlin Chinese tongue sole () is an important marine aquaculture species that exhibits pronounced female-biased sexual size dimorphism, which limits the economic value of male stocks. The F4 generation of genome-edited ZZ males carried a stable 8 bp deletion in , developed ovarian lamella-like gonads and were completely sterile, as in vitro fertilization with their gonads produced no viable embryos. From 12 to 15 months post-hatch (mph), their growth rate was significantly higher than that of wild-type males and ultimately comparable to that of females. They weighed 3.2-fold heavier and measured 1.38-fold longer than their 15 mph wild-type male counterparts. Muscle nutritional composition and most texture traits of ZZ males did not differ from wild-type females. However, their fat content was significantly lower than that of wild-type males, while their Omega6 content was significantly higher. Metabolomic analysis identified 1262 metabolites and revealed differential enrichment of pathways related to amino acids, energy, and antioxidant and neuromuscular metabolism, without evidence of detrimental shifts. Overall, editing yields a fast-growing, functionally sterile male line whose flesh quality is similar to that of wild-type tongue sole, supporting its potential use in sex-control breeding in Chinese tongue sole and providing a valuable model for studying sexual size dimorphism. |
| title | Evaluation of an Infertile, All-Male ZZ Line Exhibiting Female-like Growth in Chinese Tongue Sole (): Growth Performance, Flesh Quality, and Muscle Metabolome. |
| url | https://pubmed.ncbi.nlm.nih.gov/41514934/ |