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author Dias, Jonatan
Yen, Ling
Alcon, Francisco
Contreras, Josefa
Abrantes, Nelson
Campos, Isabel
Baldi, Isabelle
Bureau, Mathilde
Christ, Florian
Mandrioli, Daniele
Sgargi, Daria
Pasković, Igor
Pasković, Marija Polić
Glavan, Matjaž
Hofman, Jakub
Lwanga, Esperanza Huerta
Harkes, Paula
Norgaard, Trine
Aparicio, Virginia
Silva, Vera
Schlünssen, Vivi
Alaoui, Abdallah
Scheepers, Paul T J
Mol, Hans
author_facet Dias, Jonatan
Yen, Ling
Alcon, Francisco
Contreras, Josefa
Abrantes, Nelson
Campos, Isabel
Baldi, Isabelle
Bureau, Mathilde
Christ, Florian
Mandrioli, Daniele
Sgargi, Daria
Pasković, Igor
Pasković, Marija Polić
Glavan, Matjaž
Hofman, Jakub
Lwanga, Esperanza Huerta
Harkes, Paula
Norgaard, Trine
Aparicio, Virginia
Silva, Vera
Schlünssen, Vivi
Alaoui, Abdallah
Scheepers, Paul T J
Mol, Hans
Dias, Jonatan
Yen, Ling
Alcon, Francisco
Contreras, Josefa
Abrantes, Nelson
Campos, Isabel
Baldi, Isabelle
Bureau, Mathilde
Christ, Florian
Mandrioli, Daniele
Sgargi, Daria
Pasković, Igor
Pasković, Marija Polić
Glavan, Matjaž
Hofman, Jakub
Lwanga, Esperanza Huerta
Harkes, Paula
Norgaard, Trine
Aparicio, Virginia
Silva, Vera
Schlünssen, Vivi
Alaoui, Abdallah
Scheepers, Paul T J
Mol, Hans
collection PubMed - marine biology
contents Large scale biomonitoring of glyphosate and AMPA by analysis of human and animal feces and comparison with urine. Dias, Jonatan Yen, Ling Alcon, Francisco Contreras, Josefa Abrantes, Nelson Campos, Isabel Baldi, Isabelle Bureau, Mathilde Christ, Florian Mandrioli, Daniele Sgargi, Daria Pasković, Igor Pasković, Marija Polić Glavan, Matjaž Hofman, Jakub Lwanga, Esperanza Huerta Harkes, Paula Norgaard, Trine Aparicio, Virginia Silva, Vera Schlünssen, Vivi Alaoui, Abdallah Scheepers, Paul T J Mol, Hans Glyphosate Glycine Animals Humans Feces Biological Monitoring Herbicides Environmental Exposure Argentina Tetrazoles Isoxazoles alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid Environmental Pollutants Europe Internal exposure of the world's most used herbicide glyphosate and its environmental metabolite AMPA is commonly assessed by analysis of urine, while excretion is mostly through feces. In this study, we explore the feasibility and effectiveness of feces as an alternative matrix for urine for biomonitoring of these two compounds. A method for the determination of polar pesticides was validated and applied for analysis of 716 human and 249 animal feces samples. The samples were collected in 2021, at study sites in ten European countries and one in Argentina. Detection frequencies (DF) and median concentrations (MC) observed in sub-populations (conventional farmers, organic farmers, neighbors (rural), consumers (not involved in agricultural activities) were compared. Glyphosate was rather common in human feces in 71 % of the samples from the European sites and in 100 % of Argentinean samples. Detection in feces was more frequent than in the corresponding urine samples (35 % for Europe, 86 % for Argentina). MC in feces were 17.6 µg/kg (Europe) and 153 µg/kg (Argentina). Variation in DF and MC between study sites was larger than between pooled sub-populations of all study sites. In farm animals, glyphosate was found in the majority of the feces samples. AMPA was found less frequently, depending on the species and farming system. High concentrations of glyphosate and AMPA in bat feces showed that exposure also occurs at higher trophic levels in ecosystems near the investigated sites. In conclusion, analysis of feces reveals widespread exposure of both humans and animals to glyphosate, wider than so far reported based on urine as matrix.
format Artículo científico
id pubmed_41518719
institution PubMed
language en
publishDate 2026
publisher Environment international
record_format pubmed
spellingShingle Large scale biomonitoring of glyphosate and AMPA by analysis of human and animal feces and comparison with urine.
Dias, Jonatan
Yen, Ling
Alcon, Francisco
Contreras, Josefa
Abrantes, Nelson
Campos, Isabel
Baldi, Isabelle
Bureau, Mathilde
Christ, Florian
Mandrioli, Daniele
Sgargi, Daria
Pasković, Igor
Pasković, Marija Polić
Glavan, Matjaž
Hofman, Jakub
Lwanga, Esperanza Huerta
Harkes, Paula
Norgaard, Trine
Aparicio, Virginia
Silva, Vera
Schlünssen, Vivi
Alaoui, Abdallah
Scheepers, Paul T J
Mol, Hans
Glyphosate
Glycine
Animals
Humans
Feces
Biological Monitoring
Herbicides
Environmental Exposure
Argentina
Tetrazoles
Isoxazoles
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Environmental Pollutants
Europe
Large scale biomonitoring of glyphosate and AMPA by analysis of human and animal feces and comparison with urine. Dias, Jonatan Yen, Ling Alcon, Francisco Contreras, Josefa Abrantes, Nelson Campos, Isabel Baldi, Isabelle Bureau, Mathilde Christ, Florian Mandrioli, Daniele Sgargi, Daria Pasković, Igor Pasković, Marija Polić Glavan, Matjaž Hofman, Jakub Lwanga, Esperanza Huerta Harkes, Paula Norgaard, Trine Aparicio, Virginia Silva, Vera Schlünssen, Vivi Alaoui, Abdallah Scheepers, Paul T J Mol, Hans Glyphosate Glycine Animals Humans Feces Biological Monitoring Herbicides Environmental Exposure Argentina Tetrazoles Isoxazoles alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid Environmental Pollutants Europe Internal exposure of the world's most used herbicide glyphosate and its environmental metabolite AMPA is commonly assessed by analysis of urine, while excretion is mostly through feces. In this study, we explore the feasibility and effectiveness of feces as an alternative matrix for urine for biomonitoring of these two compounds. A method for the determination of polar pesticides was validated and applied for analysis of 716 human and 249 animal feces samples. The samples were collected in 2021, at study sites in ten European countries and one in Argentina. Detection frequencies (DF) and median concentrations (MC) observed in sub-populations (conventional farmers, organic farmers, neighbors (rural), consumers (not involved in agricultural activities) were compared. Glyphosate was rather common in human feces in 71 % of the samples from the European sites and in 100 % of Argentinean samples. Detection in feces was more frequent than in the corresponding urine samples (35 % for Europe, 86 % for Argentina). MC in feces were 17.6 µg/kg (Europe) and 153 µg/kg (Argentina). Variation in DF and MC between study sites was larger than between pooled sub-populations of all study sites. In farm animals, glyphosate was found in the majority of the feces samples. AMPA was found less frequently, depending on the species and farming system. High concentrations of glyphosate and AMPA in bat feces showed that exposure also occurs at higher trophic levels in ecosystems near the investigated sites. In conclusion, analysis of feces reveals widespread exposure of both humans and animals to glyphosate, wider than so far reported based on urine as matrix.
title Large scale biomonitoring of glyphosate and AMPA by analysis of human and animal feces and comparison with urine.
topic Glyphosate
Glycine
Animals
Humans
Feces
Biological Monitoring
Herbicides
Environmental Exposure
Argentina
Tetrazoles
Isoxazoles
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Environmental Pollutants
Europe
url https://pubmed.ncbi.nlm.nih.gov/41518719/