Effects of temperature on the structure and bioactivity of exopolysaccharides from Pseudoalteromonas agarivorans Hao2018: integrated transcriptomic and metabolomic analysis.
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| Format: | Artículo científico |
| Sprache: | en |
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International journal of biological macromolecules
2026
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| author | Shi, Shengbo Wang, Wei Wang, Yuanyuan Li, Jinghua Fang, Longyu Geng, Rui Ju, Yuhao Chen, Junfeng Liu, Wenlin Hao, Lujiang |
| author_facet | Shi, Shengbo Wang, Wei Wang, Yuanyuan Li, Jinghua Fang, Longyu Geng, Rui Ju, Yuhao Chen, Junfeng Liu, Wenlin Hao, Lujiang Shi, Shengbo Wang, Wei Wang, Yuanyuan Li, Jinghua Fang, Longyu Geng, Rui Ju, Yuhao Chen, Junfeng Liu, Wenlin Hao, Lujiang |
| collection | PubMed - marine biology |
| contents | Effects of temperature on the structure and bioactivity of exopolysaccharides from Pseudoalteromonas agarivorans Hao2018: integrated transcriptomic and metabolomic analysis. Shi, Shengbo Wang, Wei Wang, Yuanyuan Li, Jinghua Fang, Longyu Geng, Rui Ju, Yuhao Chen, Junfeng Liu, Wenlin Hao, Lujiang Pseudoalteromonas Polysaccharides, Bacterial Temperature Animals Metabolomics Transcriptome Gene Expression Profiling Monosaccharides Bacterial exopolysaccharides (EPS) are high-molecular-weight carbohydrate polymers synthesized and secreted by bacteria, aiding in their adaptation to environmental changes and mitigating associated damage. This study systematically investigated the effects of fermentation temperature on the structure characteristics and biological activity of EPS from Pseudoalteromonas agarivorans Hao2018 (P. agarivorans Hao2018). As the temperature increased from 15 °C to 25 °C, variations in monosaccharide composition ratios were observed, with a higher proportion of mannose and rhamnose. Correspondingly, the glycosidic bond configuration and higher-order structure becoming more compact and ordered, forming a stable three-dimensional network. EPS-25 °C exhibited enhanced free radical scavenging activity and improved anti-aging effects in a Caenorhabditis elegans model. However, when the temperature rose to 35 °C, EPS showed increased structural heterogeneity and reduced bioactivity, suggesting that elevated temperatures lead to less favorable polysaccharide structural organization. Integrated transcriptomic and metabolomic analyses revealed that temperature variations impacted the expression of genes and pathways involved in EPS synthesis, along with changes in the levels of key sugar metabolism intermediates. Notably, genes involved in dTDP-rhamnose synthesis were upregulated at 25 °C, which is consistent with the observed changes in the levels of associated metabolites. Furthermore, heterologous synthesis of dTDP-rhamnose was achieved using engineered strains via a multi-enzyme cascade reaction, demonstrating the feasibility of EPS precursor production. This study provides insight into temperature-dependent EPS synthesis and structure-function relationships in marine bacteria, and offers a basis for exploring the potential application of P. agarivorans Hao2018 EPS as a natural antioxidant. |
| format | Artículo científico |
| id | pubmed_41519345 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | International journal of biological macromolecules |
| record_format | pubmed |
| spellingShingle | Effects of temperature on the structure and bioactivity of exopolysaccharides from Pseudoalteromonas agarivorans Hao2018: integrated transcriptomic and metabolomic analysis. Shi, Shengbo Wang, Wei Wang, Yuanyuan Li, Jinghua Fang, Longyu Geng, Rui Ju, Yuhao Chen, Junfeng Liu, Wenlin Hao, Lujiang Pseudoalteromonas Polysaccharides, Bacterial Temperature Animals Metabolomics Transcriptome Gene Expression Profiling Monosaccharides Effects of temperature on the structure and bioactivity of exopolysaccharides from Pseudoalteromonas agarivorans Hao2018: integrated transcriptomic and metabolomic analysis. Shi, Shengbo Wang, Wei Wang, Yuanyuan Li, Jinghua Fang, Longyu Geng, Rui Ju, Yuhao Chen, Junfeng Liu, Wenlin Hao, Lujiang Pseudoalteromonas Polysaccharides, Bacterial Temperature Animals Metabolomics Transcriptome Gene Expression Profiling Monosaccharides Bacterial exopolysaccharides (EPS) are high-molecular-weight carbohydrate polymers synthesized and secreted by bacteria, aiding in their adaptation to environmental changes and mitigating associated damage. This study systematically investigated the effects of fermentation temperature on the structure characteristics and biological activity of EPS from Pseudoalteromonas agarivorans Hao2018 (P. agarivorans Hao2018). As the temperature increased from 15 °C to 25 °C, variations in monosaccharide composition ratios were observed, with a higher proportion of mannose and rhamnose. Correspondingly, the glycosidic bond configuration and higher-order structure becoming more compact and ordered, forming a stable three-dimensional network. EPS-25 °C exhibited enhanced free radical scavenging activity and improved anti-aging effects in a Caenorhabditis elegans model. However, when the temperature rose to 35 °C, EPS showed increased structural heterogeneity and reduced bioactivity, suggesting that elevated temperatures lead to less favorable polysaccharide structural organization. Integrated transcriptomic and metabolomic analyses revealed that temperature variations impacted the expression of genes and pathways involved in EPS synthesis, along with changes in the levels of key sugar metabolism intermediates. Notably, genes involved in dTDP-rhamnose synthesis were upregulated at 25 °C, which is consistent with the observed changes in the levels of associated metabolites. Furthermore, heterologous synthesis of dTDP-rhamnose was achieved using engineered strains via a multi-enzyme cascade reaction, demonstrating the feasibility of EPS precursor production. This study provides insight into temperature-dependent EPS synthesis and structure-function relationships in marine bacteria, and offers a basis for exploring the potential application of P. agarivorans Hao2018 EPS as a natural antioxidant. |
| title | Effects of temperature on the structure and bioactivity of exopolysaccharides from Pseudoalteromonas agarivorans Hao2018: integrated transcriptomic and metabolomic analysis. |
| topic | Pseudoalteromonas Polysaccharides, Bacterial Temperature Animals Metabolomics Transcriptome Gene Expression Profiling Monosaccharides |
| url | https://pubmed.ncbi.nlm.nih.gov/41519345/ |