Point-of-need visual test for the detection of γ-glutamyltransferase in calf serum.
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| Format: | Artículo científico |
| Sprache: | en |
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Veterinary journal (London, England : 1997)
2026
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| author | Campàs, Mònica Tolós, Martina Leonardo, Sandra Llonch, Lourdes Devant, Maria Saco, Yolanda Bassols, Anna Martí, Sònia |
| author_facet | Campàs, Mònica Tolós, Martina Leonardo, Sandra Llonch, Lourdes Devant, Maria Saco, Yolanda Bassols, Anna Martí, Sònia Campàs, Mònica Tolós, Martina Leonardo, Sandra Llonch, Lourdes Devant, Maria Saco, Yolanda Bassols, Anna Martí, Sònia |
| collection | PubMed - marine biology |
| contents | Point-of-need visual test for the detection of γ-glutamyltransferase in calf serum. Campàs, Mònica Tolós, Martina Leonardo, Sandra Llonch, Lourdes Devant, Maria Saco, Yolanda Bassols, Anna Martí, Sònia Animals gamma-Glutamyltransferase Cattle Sensitivity and Specificity Female Colostrum Point-of-Care Systems Adequate colostrum intake is critical for the passive transfer of immunity in calves, and serum γ-glutamyltransferase (GGT; EC 2.3.2.2) is a potential indicator. However, conventional assays for its detection require laboratory facilities, limiting their use in the field. Herein, a simple visual test has been developed for the semi-quantitative determination of serum GGT using immobilized reagents on paper supports. The enzyme substrate, drop volume, and membrane type were optimized, with γ-L-glutamyl-3-carboxy-4-nitroanilide, 10 µL drop volume, and a binder-free microfiber glass membrane providing the best performance. The visual test showed a limit of semi-quantification (LOSQ) of 39 U/L, while digital analysis slightly improved the sensitivity. When applied to calf serum samples, the test reliably reflected GGT evolution: negligible at birth, peak activity at 2 days (corresponding to colostrum intake), declining by day 14, and further decreasing at day 18. The visual test showed strong agreement with the conventional enzyme assay (κ = 0.94), correctly estimating 26 out of 31 samples (84 %), and digital analysis of the membranes correlated even more closely (R = 0.9924). This point-of-need (PON) device offers a rapid, low-cost, and practical tool for on-farm monitoring of colostrum intake, supporting early detection of failure of transfer of passive immunity (FTPI) and improved calf health management. |
| format | Artículo científico |
| id | pubmed_41520871 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Veterinary journal (London, England : 1997) |
| record_format | pubmed |
| spellingShingle | Point-of-need visual test for the detection of γ-glutamyltransferase in calf serum. Campàs, Mònica Tolós, Martina Leonardo, Sandra Llonch, Lourdes Devant, Maria Saco, Yolanda Bassols, Anna Martí, Sònia Animals gamma-Glutamyltransferase Cattle Sensitivity and Specificity Female Colostrum Point-of-Care Systems Point-of-need visual test for the detection of γ-glutamyltransferase in calf serum. Campàs, Mònica Tolós, Martina Leonardo, Sandra Llonch, Lourdes Devant, Maria Saco, Yolanda Bassols, Anna Martí, Sònia Animals gamma-Glutamyltransferase Cattle Sensitivity and Specificity Female Colostrum Point-of-Care Systems Adequate colostrum intake is critical for the passive transfer of immunity in calves, and serum γ-glutamyltransferase (GGT; EC 2.3.2.2) is a potential indicator. However, conventional assays for its detection require laboratory facilities, limiting their use in the field. Herein, a simple visual test has been developed for the semi-quantitative determination of serum GGT using immobilized reagents on paper supports. The enzyme substrate, drop volume, and membrane type were optimized, with γ-L-glutamyl-3-carboxy-4-nitroanilide, 10 µL drop volume, and a binder-free microfiber glass membrane providing the best performance. The visual test showed a limit of semi-quantification (LOSQ) of 39 U/L, while digital analysis slightly improved the sensitivity. When applied to calf serum samples, the test reliably reflected GGT evolution: negligible at birth, peak activity at 2 days (corresponding to colostrum intake), declining by day 14, and further decreasing at day 18. The visual test showed strong agreement with the conventional enzyme assay (κ = 0.94), correctly estimating 26 out of 31 samples (84 %), and digital analysis of the membranes correlated even more closely (R = 0.9924). This point-of-need (PON) device offers a rapid, low-cost, and practical tool for on-farm monitoring of colostrum intake, supporting early detection of failure of transfer of passive immunity (FTPI) and improved calf health management. |
| title | Point-of-need visual test for the detection of γ-glutamyltransferase in calf serum. |
| topic | Animals gamma-Glutamyltransferase Cattle Sensitivity and Specificity Female Colostrum Point-of-Care Systems |
| url | https://pubmed.ncbi.nlm.nih.gov/41520871/ |