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| Hauptverfasser: | , , , , , , , |
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| Format: | Artículo científico |
| Sprache: | en |
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Veterinary journal (London, England : 1997)
2026
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/41520871/ |
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Inhaltsangabe:
- Point-of-need visual test for the detection of γ-glutamyltransferase in calf serum. Campàs, Mònica Tolós, Martina Leonardo, Sandra Llonch, Lourdes Devant, Maria Saco, Yolanda Bassols, Anna Martí, Sònia Animals gamma-Glutamyltransferase Cattle Sensitivity and Specificity Female Colostrum Point-of-Care Systems Adequate colostrum intake is critical for the passive transfer of immunity in calves, and serum γ-glutamyltransferase (GGT; EC 2.3.2.2) is a potential indicator. However, conventional assays for its detection require laboratory facilities, limiting their use in the field. Herein, a simple visual test has been developed for the semi-quantitative determination of serum GGT using immobilized reagents on paper supports. The enzyme substrate, drop volume, and membrane type were optimized, with γ-L-glutamyl-3-carboxy-4-nitroanilide, 10 µL drop volume, and a binder-free microfiber glass membrane providing the best performance. The visual test showed a limit of semi-quantification (LOSQ) of 39 U/L, while digital analysis slightly improved the sensitivity. When applied to calf serum samples, the test reliably reflected GGT evolution: negligible at birth, peak activity at 2 days (corresponding to colostrum intake), declining by day 14, and further decreasing at day 18. The visual test showed strong agreement with the conventional enzyme assay (κ = 0.94), correctly estimating 26 out of 31 samples (84 %), and digital analysis of the membranes correlated even more closely (R = 0.9924). This point-of-need (PON) device offers a rapid, low-cost, and practical tool for on-farm monitoring of colostrum intake, supporting early detection of failure of transfer of passive immunity (FTPI) and improved calf health management.