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| Format: | Artículo científico |
| Sprache: | en |
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Ecology and evolution
2026
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/41522215/ |
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| _version_ | 1868266100236484608 |
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| author | Zhang, Yi Duan, Jiawen Zheng, Yimeng Chen, Xiaoqi Li, Chenhui Xie, Zhenyu Huang, Aiyou |
| author_facet | Zhang, Yi Duan, Jiawen Zheng, Yimeng Chen, Xiaoqi Li, Chenhui Xie, Zhenyu Huang, Aiyou Zhang, Yi Duan, Jiawen Zheng, Yimeng Chen, Xiaoqi Li, Chenhui Xie, Zhenyu Huang, Aiyou |
| collection | PubMed - marine biology |
| contents | Transcriptomic Responses of the Marine Diatom to High Carbon and Low Nitrogen Stress. Zhang, Yi Duan, Jiawen Zheng, Yimeng Chen, Xiaoqi Li, Chenhui Xie, Zhenyu Huang, Aiyou Diatoms play a pivotal role in global biogeochemical cycling and marine primary productivity, making them ideal model organisms for understanding how phytoplankton respond to environmental fluctuations associated with global climate change. In natural marine systems, diatoms frequently encounter simultaneous variations in carbon and nitrogen availability, yet most previous studies have examined the effects of these factors in isolation. To elucidate the integrated transcriptional mechanisms underlying diatom acclimation to coupled carbon-nitrogen (C-N) imbalance, we employed RNA sequencing (RNA-Seq) to characterize the global transcriptional response of the model diatom to high CO (~2000 μatm) and low nitrogen (10% of nitrogen concentration in f/2 medium) under parallel culture conditions. The results revealed both shared and distinct transcriptional responses between the two treatments. Key genes involved in carbon metabolism, such as phosphoglycerate mutase (PGAM_7) and dihydrolipoamide succinyltransferase (PHATRDRAFT_40430), were significantly upregulated, indicating enhanced glycolytic and TCA cycle activity. In contrast, the Calvin-cycle enzyme fructose-1,6-bisphosphatase (FBPC4) was downregulated. Genes associated with nitrogen assimilation-including nitrate reductase (PHATRDRAFT_54983), nitrite reductases (PHATRDRAFT_13154, PHATRDRAFT_8155), and ferredoxin-nitrite reductase (PHATRDRAFT_27757)-were strongly induced under both conditions. Pathway enrichment analysis further indicated the activation of lactic acid fermentation and nitrogen salvage pathways, suggesting a metabolic shift toward energy conservation and nutrient recycling. Collectively, these findings provide an overview of the transcriptional adjustments that enable to maintain C-N homeostasis under high CO and low nitrogen stress, offering new insights into diatom metabolic plasticity under changing ocean conditions. |
| format | Artículo científico |
| id | pubmed_41522215 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Ecology and evolution |
| record_format | pubmed |
| spellingShingle | Transcriptomic Responses of the Marine Diatom to High Carbon and Low Nitrogen Stress. Zhang, Yi Duan, Jiawen Zheng, Yimeng Chen, Xiaoqi Li, Chenhui Xie, Zhenyu Huang, Aiyou Transcriptomic Responses of the Marine Diatom to High Carbon and Low Nitrogen Stress. Zhang, Yi Duan, Jiawen Zheng, Yimeng Chen, Xiaoqi Li, Chenhui Xie, Zhenyu Huang, Aiyou Diatoms play a pivotal role in global biogeochemical cycling and marine primary productivity, making them ideal model organisms for understanding how phytoplankton respond to environmental fluctuations associated with global climate change. In natural marine systems, diatoms frequently encounter simultaneous variations in carbon and nitrogen availability, yet most previous studies have examined the effects of these factors in isolation. To elucidate the integrated transcriptional mechanisms underlying diatom acclimation to coupled carbon-nitrogen (C-N) imbalance, we employed RNA sequencing (RNA-Seq) to characterize the global transcriptional response of the model diatom to high CO (~2000 μatm) and low nitrogen (10% of nitrogen concentration in f/2 medium) under parallel culture conditions. The results revealed both shared and distinct transcriptional responses between the two treatments. Key genes involved in carbon metabolism, such as phosphoglycerate mutase (PGAM_7) and dihydrolipoamide succinyltransferase (PHATRDRAFT_40430), were significantly upregulated, indicating enhanced glycolytic and TCA cycle activity. In contrast, the Calvin-cycle enzyme fructose-1,6-bisphosphatase (FBPC4) was downregulated. Genes associated with nitrogen assimilation-including nitrate reductase (PHATRDRAFT_54983), nitrite reductases (PHATRDRAFT_13154, PHATRDRAFT_8155), and ferredoxin-nitrite reductase (PHATRDRAFT_27757)-were strongly induced under both conditions. Pathway enrichment analysis further indicated the activation of lactic acid fermentation and nitrogen salvage pathways, suggesting a metabolic shift toward energy conservation and nutrient recycling. Collectively, these findings provide an overview of the transcriptional adjustments that enable to maintain C-N homeostasis under high CO and low nitrogen stress, offering new insights into diatom metabolic plasticity under changing ocean conditions. |
| title | Transcriptomic Responses of the Marine Diatom to High Carbon and Low Nitrogen Stress. |
| url | https://pubmed.ncbi.nlm.nih.gov/41522215/ |