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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Nature communications
2026
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| Soggetti: | |
| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/41530154/ |
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| _version_ | 1868266100219707392 |
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| author | Li, Huan Liu, Fujiang Li, Jingjing Shi, Chenggang Lin, Zhenyi Qin, Yucheng Pan, Rongrong Wu, Xiaotong Peng, Yongying Xing, Chaofan Wang, Yiquan Qu, Qingming Li, Guang |
| author_facet | Li, Huan Liu, Fujiang Li, Jingjing Shi, Chenggang Lin, Zhenyi Qin, Yucheng Pan, Rongrong Wu, Xiaotong Peng, Yongying Xing, Chaofan Wang, Yiquan Qu, Qingming Li, Guang Li, Huan Liu, Fujiang Li, Jingjing Shi, Chenggang Lin, Zhenyi Qin, Yucheng Pan, Rongrong Wu, Xiaotong Peng, Yongying Xing, Chaofan Wang, Yiquan Qu, Qingming Li, Guang |
| collection | PubMed - marine biology |
| contents | Evolutionary dynamics of sex determination in Branchiostoma belcheri driven by repeated transposition of a single novel gene. Li, Huan Liu, Fujiang Li, Jingjing Shi, Chenggang Lin, Zhenyi Qin, Yucheng Pan, Rongrong Wu, Xiaotong Peng, Yongying Xing, Chaofan Wang, Yiquan Qu, Qingming Li, Guang Animals Sex Determination Processes Lancelets Female Male Evolution, Molecular DNA Transposable Elements CRISPR-Cas Systems Sex determination systems display striking evolutionary flexibility, yet the mechanisms underlying their transitions remain poorly understood. Using newly generated genome assemblies, we investigated the evolving sex-determining system in the amphioxus Branchiostoma belcheri. We identified two female-specific sex-determining regions (SDRs) on chromosome 13, both derived from independent transpositions of the autosomal gene tesD, which shows testis-specific expression in amphioxus species. CRISPR/Cas9 knockout experiments in Branchiostoma floridae confirmed that tesD functions as a male-determination gene, with loss of function producing an all-female phenotype. In B. belcheri, the older SDR (tesDwa) inserted into the coding region of twai, while the younger SDR (tesDwb), flanked by active Zator-1 transposons, inserted into the 3' UTR of vps9c and later translocated to autosomes in ~10% of individuals. Transcriptomic analyses revealed that W-linked tesDwa and tesDwb produce antisense long non-coding RNAs that likely suppress tesD transcription in females, whereas autosomal tesDwb is not expressed and appears non-functional. The insertion sites and co-transcription with host genes suggest promoter hijacking. Together, these findings demonstrate that recurrent transpositions can generate new functional SDRs that coexist with older ones, driving dynamic turnover of sex determination in B. belcheri. |
| format | Artículo científico |
| id | pubmed_41530154 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Nature communications |
| record_format | pubmed |
| spellingShingle | Evolutionary dynamics of sex determination in Branchiostoma belcheri driven by repeated transposition of a single novel gene. Li, Huan Liu, Fujiang Li, Jingjing Shi, Chenggang Lin, Zhenyi Qin, Yucheng Pan, Rongrong Wu, Xiaotong Peng, Yongying Xing, Chaofan Wang, Yiquan Qu, Qingming Li, Guang Animals Sex Determination Processes Lancelets Female Male Evolution, Molecular DNA Transposable Elements CRISPR-Cas Systems Evolutionary dynamics of sex determination in Branchiostoma belcheri driven by repeated transposition of a single novel gene. Li, Huan Liu, Fujiang Li, Jingjing Shi, Chenggang Lin, Zhenyi Qin, Yucheng Pan, Rongrong Wu, Xiaotong Peng, Yongying Xing, Chaofan Wang, Yiquan Qu, Qingming Li, Guang Animals Sex Determination Processes Lancelets Female Male Evolution, Molecular DNA Transposable Elements CRISPR-Cas Systems Sex determination systems display striking evolutionary flexibility, yet the mechanisms underlying their transitions remain poorly understood. Using newly generated genome assemblies, we investigated the evolving sex-determining system in the amphioxus Branchiostoma belcheri. We identified two female-specific sex-determining regions (SDRs) on chromosome 13, both derived from independent transpositions of the autosomal gene tesD, which shows testis-specific expression in amphioxus species. CRISPR/Cas9 knockout experiments in Branchiostoma floridae confirmed that tesD functions as a male-determination gene, with loss of function producing an all-female phenotype. In B. belcheri, the older SDR (tesDwa) inserted into the coding region of twai, while the younger SDR (tesDwb), flanked by active Zator-1 transposons, inserted into the 3' UTR of vps9c and later translocated to autosomes in ~10% of individuals. Transcriptomic analyses revealed that W-linked tesDwa and tesDwb produce antisense long non-coding RNAs that likely suppress tesD transcription in females, whereas autosomal tesDwb is not expressed and appears non-functional. The insertion sites and co-transcription with host genes suggest promoter hijacking. Together, these findings demonstrate that recurrent transpositions can generate new functional SDRs that coexist with older ones, driving dynamic turnover of sex determination in B. belcheri. |
| title | Evolutionary dynamics of sex determination in Branchiostoma belcheri driven by repeated transposition of a single novel gene. |
| topic | Animals Sex Determination Processes Lancelets Female Male Evolution, Molecular DNA Transposable Elements CRISPR-Cas Systems |
| url | https://pubmed.ncbi.nlm.nih.gov/41530154/ |