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| Hauptverfasser: | , , , , , |
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| Format: | Artículo científico |
| Sprache: | en |
| Veröffentlicht: |
Frontiers in toxicology
2025
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/41561475/ |
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Inhaltsangabe:
- Comparative assessment of solvents toxicity using early life stages of amphibians and cell lines: a case study with dimethyl sulfoxide. Coelho, Sónia Dias Campos, Diana Almeida, Mónica Quintaneiro, Carla Oliveira, Miguel Lopes, Isabel The reduction in the number of animals being used in experimental assays has been a concern of the scientific community. In this sense, non-animal alternative methods have been increasingly tested. This study intended to explore how cell-based responses compare to organismal outcomes and if the former models could contribute to minimizing the number of live animals needed in subsequent stages of hazard/risk assessment of chemicals on amphibians. For this, the toxicity of the commonly used solvent dimethyl sulfoxide (DMSO) was assessed in early life stages (embryos and tadpoles) of two anuran species ( and ) and in 2 cell lines of (A6 and XTC-2). In the assays, mortality, teratogenic effects, and biometric parameters were evaluated, while for assays, the assessed endpoint was viability. Overall, the obtained data suggest similar sensitivity of both species and life stages to DMSO. The 96 h-LC estimated for embryos and tadpoles were, respectively, 2.19% and 2.56% for and 3.19 and 3.41 for . The solvent DMSO induced several malformations in early life stages, which may have implications for the fitness of organisms at later stages. A slightly higher sensitivity to DMSO was observed in the approaches comparatively to approach (72 h-LC of 3.10% and 2.62% for A6 and XTC-2, respectively), though it can not be considered significantly different. As such, it is suggested that the latter approach may be considered to serve for first screenings of the ecotoxicity of organic solvents. Such a strategy of using assays as screening tools, has the potential to reduce the number of animals to be used in subsequent testing phases by providing information for the refinement of concentrations to be tested in assays, thereby supporting both reduction and replacement objectives.