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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Fish & shellfish immunology
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41565087/ |
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| _version_ | 1868266097666424833 |
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| author | Feng, Jianhua Wang, Yafang Huang, Wenji Xie, Dawei Huang, Yinghao Tao, Xinran Zhang, Tiantian Wu, Haixia Zou, Jun Wang, Junya |
| author_facet | Feng, Jianhua Wang, Yafang Huang, Wenji Xie, Dawei Huang, Yinghao Tao, Xinran Zhang, Tiantian Wu, Haixia Zou, Jun Wang, Junya Feng, Jianhua Wang, Yafang Huang, Wenji Xie, Dawei Huang, Yinghao Tao, Xinran Zhang, Tiantian Wu, Haixia Zou, Jun Wang, Junya |
| collection | PubMed - marine biology |
| contents | RBX1 and RBX2 promote GCRV replication in grass carp (Ctenopharyngodon idella). Feng, Jianhua Wang, Yafang Huang, Wenji Xie, Dawei Huang, Yinghao Tao, Xinran Zhang, Tiantian Wu, Haixia Zou, Jun Wang, Junya Animals Carps Fish Proteins Immunity, Innate Virus Replication Fish Diseases Phylogeny Reoviridae Reoviridae Infections Rhabdoviridae Sequence Alignment Gene Expression Regulation Ubiquitin-Protein Ligases Amino Acid Sequence Rhabdoviridae Infections Cullin-RING ligases (CRLs) constitute the most diverse E3 ligase family in the ubiquitin-proteasome system, yet their antiviral roles in bony fish remain poorly understood. Here, we identify the catalytic cores of CRLs, the RING-box proteins RBX1 and RBX2, as key modulators of antiviral signaling in cyprinid fish. Phylogenetic and structural analyses revealed that Rbx1 and Rbx2 are highly conserved and widely expressed, with preferential enrichment in primordial germ and immune-related cells. Upon infection with grass carp reovirus (GCRV) or spring viremia of carp virus (SVCV) in cyprinid fish, Rbxs were rapidly induced in vivo and in vitro. RBX1 and RBX2 synergistically promote viral replication by directly interacting with interferon regulatory factors (IRF) 3 and IRF7 and facilitating their ubiquitin-mediated regulation. Together, our findings uncover RBX1 and RBX2 as evolutionarily conserved negative regulators of fish innate immunity and provide mechanistic insight into the ubiquitin-mediated control of interferon homeostasis across vertebrates. |
| format | Artículo científico |
| id | pubmed_41565087 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Fish & shellfish immunology |
| record_format | pubmed |
| spellingShingle | RBX1 and RBX2 promote GCRV replication in grass carp (Ctenopharyngodon idella). Feng, Jianhua Wang, Yafang Huang, Wenji Xie, Dawei Huang, Yinghao Tao, Xinran Zhang, Tiantian Wu, Haixia Zou, Jun Wang, Junya Animals Carps Fish Proteins Immunity, Innate Virus Replication Fish Diseases Phylogeny Reoviridae Reoviridae Infections Rhabdoviridae Sequence Alignment Gene Expression Regulation Ubiquitin-Protein Ligases Amino Acid Sequence Rhabdoviridae Infections RBX1 and RBX2 promote GCRV replication in grass carp (Ctenopharyngodon idella). Feng, Jianhua Wang, Yafang Huang, Wenji Xie, Dawei Huang, Yinghao Tao, Xinran Zhang, Tiantian Wu, Haixia Zou, Jun Wang, Junya Animals Carps Fish Proteins Immunity, Innate Virus Replication Fish Diseases Phylogeny Reoviridae Reoviridae Infections Rhabdoviridae Sequence Alignment Gene Expression Regulation Ubiquitin-Protein Ligases Amino Acid Sequence Rhabdoviridae Infections Cullin-RING ligases (CRLs) constitute the most diverse E3 ligase family in the ubiquitin-proteasome system, yet their antiviral roles in bony fish remain poorly understood. Here, we identify the catalytic cores of CRLs, the RING-box proteins RBX1 and RBX2, as key modulators of antiviral signaling in cyprinid fish. Phylogenetic and structural analyses revealed that Rbx1 and Rbx2 are highly conserved and widely expressed, with preferential enrichment in primordial germ and immune-related cells. Upon infection with grass carp reovirus (GCRV) or spring viremia of carp virus (SVCV) in cyprinid fish, Rbxs were rapidly induced in vivo and in vitro. RBX1 and RBX2 synergistically promote viral replication by directly interacting with interferon regulatory factors (IRF) 3 and IRF7 and facilitating their ubiquitin-mediated regulation. Together, our findings uncover RBX1 and RBX2 as evolutionarily conserved negative regulators of fish innate immunity and provide mechanistic insight into the ubiquitin-mediated control of interferon homeostasis across vertebrates. |
| title | RBX1 and RBX2 promote GCRV replication in grass carp (Ctenopharyngodon idella). |
| topic | Animals Carps Fish Proteins Immunity, Innate Virus Replication Fish Diseases Phylogeny Reoviridae Reoviridae Infections Rhabdoviridae Sequence Alignment Gene Expression Regulation Ubiquitin-Protein Ligases Amino Acid Sequence Rhabdoviridae Infections |
| url | https://pubmed.ncbi.nlm.nih.gov/41565087/ |