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Main Authors: Díaz, Francisco, Inoubli, Sheyma, Queffelec, Julie, González-Martínez, Noa, Kurman, Kelly V, Almeida, Adelaide, Silva-Reis, Rita, Vieira, Catia, Díaz-Reinoso, Beatriz, Flórez-Fernández, Noelia, Torres, M Dolores, Piñeiro-Lago, Beatriz, Cardoso, Susana M, Domínguez, Herminia
Format: Artículo científico
Language:en
Published: Analytical and bioanalytical chemistry 2026
Subjects:
Online Access:https://pubmed.ncbi.nlm.nih.gov/41569418/
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author Díaz, Francisco
Inoubli, Sheyma
Queffelec, Julie
González-Martínez, Noa
Kurman, Kelly V
Almeida, Adelaide
Silva-Reis, Rita
Vieira, Catia
Díaz-Reinoso, Beatriz
Flórez-Fernández, Noelia
Torres, M Dolores
Piñeiro-Lago, Beatriz
Cardoso, Susana M
Domínguez, Herminia
author_facet Díaz, Francisco
Inoubli, Sheyma
Queffelec, Julie
González-Martínez, Noa
Kurman, Kelly V
Almeida, Adelaide
Silva-Reis, Rita
Vieira, Catia
Díaz-Reinoso, Beatriz
Flórez-Fernández, Noelia
Torres, M Dolores
Piñeiro-Lago, Beatriz
Cardoso, Susana M
Domínguez, Herminia
Díaz, Francisco
Inoubli, Sheyma
Queffelec, Julie
González-Martínez, Noa
Kurman, Kelly V
Almeida, Adelaide
Silva-Reis, Rita
Vieira, Catia
Díaz-Reinoso, Beatriz
Flórez-Fernández, Noelia
Torres, M Dolores
Piñeiro-Lago, Beatriz
Cardoso, Susana M
Domínguez, Herminia
collection PubMed - marine biology
contents Microwave hydrodiffusion and gravity pretreatment of Camellia japonica flowers for the extraction of bioactives. Díaz, Francisco Inoubli, Sheyma Queffelec, Julie González-Martínez, Noa Kurman, Kelly V Almeida, Adelaide Silva-Reis, Rita Vieira, Catia Díaz-Reinoso, Beatriz Flórez-Fernández, Noelia Torres, M Dolores Piñeiro-Lago, Beatriz Cardoso, Susana M Domínguez, Herminia Microwaves Camellia Flowers Antioxidants Plant Extracts Anti-Bacterial Agents Phenols Diffusion Staphylococcus aureus The valorization of Camellia japonica flower varieties through the green extraction of their bioactive components is addressed. Microwave hydrodiffusion and gravity (MHG) can be an efficient and rapid pretreatment to dry the petals; for example, in the Royal Velvet variety, the energy consumption was 16 times lower compared to air drying. The hydrolates obtained in this stage represented only 0.5 mg gallic acid equivalents/g flower, but some of the fractions contained up to 10 times higher and a Trolox equivalent antioxidant capacity (TEAC) value equivalent to 0.4 g Trolox/g extract. The dried solids were extracted using 96% ethanol to yield around 30% solubles, which was 50% higher than the extraction yield from the air-dried samples. The MHG dried solids were treated with supercritical CO to yield up to 2.4% of an extract containing phenolics and lipids with a favorable omega-3 to omega-6 ratio. The residual solids from supercritical extraction were further processed with microwave heated pressurized hot water for 5 min at various temperatures. The 200 °C extract achieved a solubilization of 50% of the material, obtaining a product with 22% phenolic content, almost 30% of the antiradical capacity of Trolox, a minimum inhibitory concentration (MIC) value of 8 mg/mL against Staphylococcus aureus, and contained mainly organic acids, tannins like (epi)catechin gallate and B-type (epi)catechin oligomers, and prodelphinidins like (epi)gallocatechin-O-hexoside and (epi)catechin-(epi)gallocatechin gallate. The obtained extracts were proposed for incorporation onto starch films. The results confirm the potential of non-toxic solvents to achieve a complete valorization of this resource.
format Artículo científico
id pubmed_41569418
institution PubMed
language en
publishDate 2026
publisher Analytical and bioanalytical chemistry
record_format pubmed
spellingShingle Microwave hydrodiffusion and gravity pretreatment of Camellia japonica flowers for the extraction of bioactives.
Díaz, Francisco
Inoubli, Sheyma
Queffelec, Julie
González-Martínez, Noa
Kurman, Kelly V
Almeida, Adelaide
Silva-Reis, Rita
Vieira, Catia
Díaz-Reinoso, Beatriz
Flórez-Fernández, Noelia
Torres, M Dolores
Piñeiro-Lago, Beatriz
Cardoso, Susana M
Domínguez, Herminia
Microwaves
Camellia
Flowers
Antioxidants
Plant Extracts
Anti-Bacterial Agents
Phenols
Diffusion
Staphylococcus aureus
Microwave hydrodiffusion and gravity pretreatment of Camellia japonica flowers for the extraction of bioactives. Díaz, Francisco Inoubli, Sheyma Queffelec, Julie González-Martínez, Noa Kurman, Kelly V Almeida, Adelaide Silva-Reis, Rita Vieira, Catia Díaz-Reinoso, Beatriz Flórez-Fernández, Noelia Torres, M Dolores Piñeiro-Lago, Beatriz Cardoso, Susana M Domínguez, Herminia Microwaves Camellia Flowers Antioxidants Plant Extracts Anti-Bacterial Agents Phenols Diffusion Staphylococcus aureus The valorization of Camellia japonica flower varieties through the green extraction of their bioactive components is addressed. Microwave hydrodiffusion and gravity (MHG) can be an efficient and rapid pretreatment to dry the petals; for example, in the Royal Velvet variety, the energy consumption was 16 times lower compared to air drying. The hydrolates obtained in this stage represented only 0.5 mg gallic acid equivalents/g flower, but some of the fractions contained up to 10 times higher and a Trolox equivalent antioxidant capacity (TEAC) value equivalent to 0.4 g Trolox/g extract. The dried solids were extracted using 96% ethanol to yield around 30% solubles, which was 50% higher than the extraction yield from the air-dried samples. The MHG dried solids were treated with supercritical CO to yield up to 2.4% of an extract containing phenolics and lipids with a favorable omega-3 to omega-6 ratio. The residual solids from supercritical extraction were further processed with microwave heated pressurized hot water for 5 min at various temperatures. The 200 °C extract achieved a solubilization of 50% of the material, obtaining a product with 22% phenolic content, almost 30% of the antiradical capacity of Trolox, a minimum inhibitory concentration (MIC) value of 8 mg/mL against Staphylococcus aureus, and contained mainly organic acids, tannins like (epi)catechin gallate and B-type (epi)catechin oligomers, and prodelphinidins like (epi)gallocatechin-O-hexoside and (epi)catechin-(epi)gallocatechin gallate. The obtained extracts were proposed for incorporation onto starch films. The results confirm the potential of non-toxic solvents to achieve a complete valorization of this resource.
title Microwave hydrodiffusion and gravity pretreatment of Camellia japonica flowers for the extraction of bioactives.
topic Microwaves
Camellia
Flowers
Antioxidants
Plant Extracts
Anti-Bacterial Agents
Phenols
Diffusion
Staphylococcus aureus
url https://pubmed.ncbi.nlm.nih.gov/41569418/