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| Auteurs principaux: | , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
| Publié: |
Journal of hazardous materials
2026
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| Sujets: | |
| Accès en ligne: | https://pubmed.ncbi.nlm.nih.gov/41581399/ |
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| _version_ | 1868266095173959681 |
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| author | Abuduaini, Munire Sang, Yuxuan Xie, Zhangzhang Zhang, Yuechao Wang, Oumei Li, Liangyan Liu, Fanghua |
| author_facet | Abuduaini, Munire Sang, Yuxuan Xie, Zhangzhang Zhang, Yuechao Wang, Oumei Li, Liangyan Liu, Fanghua Abuduaini, Munire Sang, Yuxuan Xie, Zhangzhang Zhang, Yuechao Wang, Oumei Li, Liangyan Liu, Fanghua |
| collection | PubMed - marine biology |
| contents | Mechanistic insights into the efficient degradation and detoxification of chloramphenicol by Clostridium pasteurianum DSM 525. Abuduaini, Munire Sang, Yuxuan Xie, Zhangzhang Zhang, Yuechao Wang, Oumei Li, Liangyan Liu, Fanghua Clostridium Inactivation, Metabolic Chloramphenicol Environmental Pollutants Biodegradation, Environmental Gene Expression Regulation, Bacterial Metabolic Networks and Pathways Chloramphenicol (CAP), a chlorinated nitroaromatic antibiotic, posed significant ecological and human health risks due to its persistence and toxicity. Although microbial degradation represented a promising remediation strategy, its practical application was often constrained by slow degradation kinetics and incomplete biotransformation. In this study, rapid and efficient CAP degradation by the Gram-positive anaerobe Clostridium pasteurianum DSM 525 was demonstrated. The strain achieved 83.6 % removal of 400 mg L CAP within 8 h, and the degradation kinetics followed a first-order model (R ≥ 0.91). Metabolite analysis revealed a nitroreduction pathway, with CAP initially transformed into 4-aminophenyl allyl alcohol and subsequently converted into a dichlorinated aromatic amine (AMCl). Integrated transcriptomic analysis uncovered a coordinated cellular response underlying the high degradation efficiency. Genes involved in the pentose phosphate pathway were significantly up-regulated, indicating enhanced NADPH supply. In parallel, a nitroreductase gene (logFC = 2.43) responsible for antibiotic reduction and an EamA family transporter potentially involved in CAP uptake (logFC = 2.07) were markedly induced, together with the up-regulation of genes encoding FF-ATP synthase, which was likely involved in ATP-dependent proton translocation and maintenance of transmembrane electrochemical gradients to support substrate transport. Concurrently, global stress-response mechanisms were activated to alleviate CAP-induced cellular stress. Collectively, this study elucidated the metabolic coordination underlying efficient CAP biodegradation and highlighted the potential of anaerobic Gram-positive bacteria for the bioremediation of nitroaromatic antibiotics. |
| format | Artículo científico |
| id | pubmed_41581399 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Journal of hazardous materials |
| record_format | pubmed |
| spellingShingle | Mechanistic insights into the efficient degradation and detoxification of chloramphenicol by Clostridium pasteurianum DSM 525. Abuduaini, Munire Sang, Yuxuan Xie, Zhangzhang Zhang, Yuechao Wang, Oumei Li, Liangyan Liu, Fanghua Clostridium Inactivation, Metabolic Chloramphenicol Environmental Pollutants Biodegradation, Environmental Gene Expression Regulation, Bacterial Metabolic Networks and Pathways Mechanistic insights into the efficient degradation and detoxification of chloramphenicol by Clostridium pasteurianum DSM 525. Abuduaini, Munire Sang, Yuxuan Xie, Zhangzhang Zhang, Yuechao Wang, Oumei Li, Liangyan Liu, Fanghua Clostridium Inactivation, Metabolic Chloramphenicol Environmental Pollutants Biodegradation, Environmental Gene Expression Regulation, Bacterial Metabolic Networks and Pathways Chloramphenicol (CAP), a chlorinated nitroaromatic antibiotic, posed significant ecological and human health risks due to its persistence and toxicity. Although microbial degradation represented a promising remediation strategy, its practical application was often constrained by slow degradation kinetics and incomplete biotransformation. In this study, rapid and efficient CAP degradation by the Gram-positive anaerobe Clostridium pasteurianum DSM 525 was demonstrated. The strain achieved 83.6 % removal of 400 mg L CAP within 8 h, and the degradation kinetics followed a first-order model (R ≥ 0.91). Metabolite analysis revealed a nitroreduction pathway, with CAP initially transformed into 4-aminophenyl allyl alcohol and subsequently converted into a dichlorinated aromatic amine (AMCl). Integrated transcriptomic analysis uncovered a coordinated cellular response underlying the high degradation efficiency. Genes involved in the pentose phosphate pathway were significantly up-regulated, indicating enhanced NADPH supply. In parallel, a nitroreductase gene (logFC = 2.43) responsible for antibiotic reduction and an EamA family transporter potentially involved in CAP uptake (logFC = 2.07) were markedly induced, together with the up-regulation of genes encoding FF-ATP synthase, which was likely involved in ATP-dependent proton translocation and maintenance of transmembrane electrochemical gradients to support substrate transport. Concurrently, global stress-response mechanisms were activated to alleviate CAP-induced cellular stress. Collectively, this study elucidated the metabolic coordination underlying efficient CAP biodegradation and highlighted the potential of anaerobic Gram-positive bacteria for the bioremediation of nitroaromatic antibiotics. |
| title | Mechanistic insights into the efficient degradation and detoxification of chloramphenicol by Clostridium pasteurianum DSM 525. |
| topic | Clostridium Inactivation, Metabolic Chloramphenicol Environmental Pollutants Biodegradation, Environmental Gene Expression Regulation, Bacterial Metabolic Networks and Pathways |
| url | https://pubmed.ncbi.nlm.nih.gov/41581399/ |