COX-2 downregulation via G-quadruplex structure induction in the PTGS2-promoter region by mononuclear octahedral cobalt(III) Schiff base complex [CoL] in colorectal cancer cells.
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Biochimica et biophysica acta. General subjects
2026
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| _version_ | 1868266095173959680 |
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| author | Moshtaghian, Abdolvahab Colagar, Abasalt Hosseinzadeh Khaleghian, Ali Zahedi, Tahereh |
| author_facet | Moshtaghian, Abdolvahab Colagar, Abasalt Hosseinzadeh Khaleghian, Ali Zahedi, Tahereh Moshtaghian, Abdolvahab Colagar, Abasalt Hosseinzadeh Khaleghian, Ali Zahedi, Tahereh |
| collection | PubMed - marine biology |
| contents | COX-2 downregulation via G-quadruplex structure induction in the PTGS2-promoter region by mononuclear octahedral cobalt(III) Schiff base complex [CoL] in colorectal cancer cells. Moshtaghian, Abdolvahab Colagar, Abasalt Hosseinzadeh Khaleghian, Ali Zahedi, Tahereh Humans Cyclooxygenase 2 G-Quadruplexes Promoter Regions, Genetic Cobalt Schiff Bases Down-Regulation Colorectal Neoplasms Molecular Docking Simulation HT29 Cells Coordination Complexes G-quadruplex structures within the promoter regions of some oncogenes diminish transcriptional activity. The suppression and downregulation of Cyclooxygenase-2 (COX-2), which is encoded by the prostaglandin-endoperoxide synthase 2 (PTGS2) gene, could control the size of colorectal cancer tumors. This study aimed to investigate the impact of a mononuclear octahedral cobalt(III) Schiff base complex [CoL] (L = 2-((allylimino)methyl)-6-methoxyphenol) on the G-quadruplex structures in the PTGS2 promoter region and to assess its downregulation effects in the human colorectal cancer cell line HT-29. At first, molecular docking was used to evaluate the binding of [CoL] to the PTGS2 promoter region. Then, the potential and stabilization of G-quadruplex formation in the PTGS2 promoter motifs were investigated using circular dichroism (CD) spectroscopy, polyacrylamide gel electrophoresis, and polymerase chain reaction (PCR) stop assays. COX-2 expression was also assessed by real-time PCR and western blot. Molecular docking analysis indicates that [CoL] has a potent interaction with the PTGS2 promoter region. Additionally, [CoL] has an intense inhibitory effect on the PTGS2-PCR stop assay. The CD measurements confirmed the conformational property of G-quadruplex DNA structures induced by [CoL]. Treatment of colorectal cancer cells with the [CoL] resulted in a modest increase in PTGS2 expression by a mean factor of 1.69 (p |
| format | Artículo científico |
| id | pubmed_41581627 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Biochimica et biophysica acta. General subjects |
| record_format | pubmed |
| spellingShingle | COX-2 downregulation via G-quadruplex structure induction in the PTGS2-promoter region by mononuclear octahedral cobalt(III) Schiff base complex [CoL] in colorectal cancer cells. Moshtaghian, Abdolvahab Colagar, Abasalt Hosseinzadeh Khaleghian, Ali Zahedi, Tahereh Humans Cyclooxygenase 2 G-Quadruplexes Promoter Regions, Genetic Cobalt Schiff Bases Down-Regulation Colorectal Neoplasms Molecular Docking Simulation HT29 Cells Coordination Complexes COX-2 downregulation via G-quadruplex structure induction in the PTGS2-promoter region by mononuclear octahedral cobalt(III) Schiff base complex [CoL] in colorectal cancer cells. Moshtaghian, Abdolvahab Colagar, Abasalt Hosseinzadeh Khaleghian, Ali Zahedi, Tahereh Humans Cyclooxygenase 2 G-Quadruplexes Promoter Regions, Genetic Cobalt Schiff Bases Down-Regulation Colorectal Neoplasms Molecular Docking Simulation HT29 Cells Coordination Complexes G-quadruplex structures within the promoter regions of some oncogenes diminish transcriptional activity. The suppression and downregulation of Cyclooxygenase-2 (COX-2), which is encoded by the prostaglandin-endoperoxide synthase 2 (PTGS2) gene, could control the size of colorectal cancer tumors. This study aimed to investigate the impact of a mononuclear octahedral cobalt(III) Schiff base complex [CoL] (L = 2-((allylimino)methyl)-6-methoxyphenol) on the G-quadruplex structures in the PTGS2 promoter region and to assess its downregulation effects in the human colorectal cancer cell line HT-29. At first, molecular docking was used to evaluate the binding of [CoL] to the PTGS2 promoter region. Then, the potential and stabilization of G-quadruplex formation in the PTGS2 promoter motifs were investigated using circular dichroism (CD) spectroscopy, polyacrylamide gel electrophoresis, and polymerase chain reaction (PCR) stop assays. COX-2 expression was also assessed by real-time PCR and western blot. Molecular docking analysis indicates that [CoL] has a potent interaction with the PTGS2 promoter region. Additionally, [CoL] has an intense inhibitory effect on the PTGS2-PCR stop assay. The CD measurements confirmed the conformational property of G-quadruplex DNA structures induced by [CoL]. Treatment of colorectal cancer cells with the [CoL] resulted in a modest increase in PTGS2 expression by a mean factor of 1.69 (p |
| title | COX-2 downregulation via G-quadruplex structure induction in the PTGS2-promoter region by mononuclear octahedral cobalt(III) Schiff base complex [CoL] in colorectal cancer cells. |
| topic | Humans Cyclooxygenase 2 G-Quadruplexes Promoter Regions, Genetic Cobalt Schiff Bases Down-Regulation Colorectal Neoplasms Molecular Docking Simulation HT29 Cells Coordination Complexes |
| url | https://pubmed.ncbi.nlm.nih.gov/41581627/ |