Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Medicina (Kaunas, Lithuania)
2026
|
| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41597504/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266092686737408 |
|---|---|
| author | Susanto, Feri Riyanti Syakuri, Hamdan Nursid, Muhammad Schäberle, Till F Mettal, Ute Choi, Jae-Suk Meinita, Maria Dyah Nur |
| author_facet | Susanto, Feri Riyanti Syakuri, Hamdan Nursid, Muhammad Schäberle, Till F Mettal, Ute Choi, Jae-Suk Meinita, Maria Dyah Nur Susanto, Feri Riyanti Syakuri, Hamdan Nursid, Muhammad Schäberle, Till F Mettal, Ute Choi, Jae-Suk Meinita, Maria Dyah Nur |
| collection | PubMed - marine biology |
| contents | Untargeted LC-HRMS-Based Metabolomic and Antibacterial Potential of Against Multidrug-Resistant Bacteria. Susanto, Feri Riyanti Syakuri, Hamdan Nursid, Muhammad Schäberle, Till F Mettal, Ute Choi, Jae-Suk Meinita, Maria Dyah Nur Sargassum Anti-Bacterial Agents Metabolomics Drug Resistance, Multiple, Bacterial Chromatography, Liquid Microbial Sensitivity Tests Liquid Chromatography-Mass Spectrometry Humans The rise in antimicrobial resistance is one of the major challenges to global health systems, which necessitates the development of new antibacterial compounds. The bioactive compounds of brown seaweed have demonstrated potential antibacterial activity. This study applied metabolomic profiling and molecular networking in combination with antibacterial screening assays to assess the antimicrobial properties of extracts against multidrug-resistant bacteria. Two extraction methods, i.e., maceration and microwave extraction, were used. Therewith, untargeted metabolomic profiling was performed using Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS). Molecular networks (MNs) were established and compound dereplication was conducted using the spectral database of the Global Natural Products Social Molecular Networking platform (GNPS). Additionally, antimicrobial assays were conducted against Gram-positive and Gram-negative bacterial strains, including multidrug-resistant bacteria, i.e., methicillin-resistant ATCC 33592 (MRSA) and β-lactamase, producing ATCC 35218 (TEM-1 positive strain). Dereplication resulted in the prediction of six compounds with reported antimicrobial properties, i.e., 13-docosenamide, 9-octadecenamide, pheophorbide A, ouabain, sarmentoside B and AC1L1X1Z. Antibacterial screening of the extracts revealed that the ethyl acetate maceration extracts exhibited the strongest inhibitory activity, with inhibition values between 85 and 98% against ATCC 33592. This metabolomics study requires further research to isolate, purify, confirm, and validate the dereplicated compounds that may have potential antibacterial activity. |
| format | Artículo científico |
| id | pubmed_41597504 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Medicina (Kaunas, Lithuania) |
| record_format | pubmed |
| spellingShingle | Untargeted LC-HRMS-Based Metabolomic and Antibacterial Potential of Against Multidrug-Resistant Bacteria. Susanto, Feri Riyanti Syakuri, Hamdan Nursid, Muhammad Schäberle, Till F Mettal, Ute Choi, Jae-Suk Meinita, Maria Dyah Nur Sargassum Anti-Bacterial Agents Metabolomics Drug Resistance, Multiple, Bacterial Chromatography, Liquid Microbial Sensitivity Tests Liquid Chromatography-Mass Spectrometry Humans Untargeted LC-HRMS-Based Metabolomic and Antibacterial Potential of Against Multidrug-Resistant Bacteria. Susanto, Feri Riyanti Syakuri, Hamdan Nursid, Muhammad Schäberle, Till F Mettal, Ute Choi, Jae-Suk Meinita, Maria Dyah Nur Sargassum Anti-Bacterial Agents Metabolomics Drug Resistance, Multiple, Bacterial Chromatography, Liquid Microbial Sensitivity Tests Liquid Chromatography-Mass Spectrometry Humans The rise in antimicrobial resistance is one of the major challenges to global health systems, which necessitates the development of new antibacterial compounds. The bioactive compounds of brown seaweed have demonstrated potential antibacterial activity. This study applied metabolomic profiling and molecular networking in combination with antibacterial screening assays to assess the antimicrobial properties of extracts against multidrug-resistant bacteria. Two extraction methods, i.e., maceration and microwave extraction, were used. Therewith, untargeted metabolomic profiling was performed using Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS). Molecular networks (MNs) were established and compound dereplication was conducted using the spectral database of the Global Natural Products Social Molecular Networking platform (GNPS). Additionally, antimicrobial assays were conducted against Gram-positive and Gram-negative bacterial strains, including multidrug-resistant bacteria, i.e., methicillin-resistant ATCC 33592 (MRSA) and β-lactamase, producing ATCC 35218 (TEM-1 positive strain). Dereplication resulted in the prediction of six compounds with reported antimicrobial properties, i.e., 13-docosenamide, 9-octadecenamide, pheophorbide A, ouabain, sarmentoside B and AC1L1X1Z. Antibacterial screening of the extracts revealed that the ethyl acetate maceration extracts exhibited the strongest inhibitory activity, with inhibition values between 85 and 98% against ATCC 33592. This metabolomics study requires further research to isolate, purify, confirm, and validate the dereplicated compounds that may have potential antibacterial activity. |
| title | Untargeted LC-HRMS-Based Metabolomic and Antibacterial Potential of Against Multidrug-Resistant Bacteria. |
| topic | Sargassum Anti-Bacterial Agents Metabolomics Drug Resistance, Multiple, Bacterial Chromatography, Liquid Microbial Sensitivity Tests Liquid Chromatography-Mass Spectrometry Humans |
| url | https://pubmed.ncbi.nlm.nih.gov/41597504/ |