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| Auteurs principaux: | , , , , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
| Publié: |
Microorganisms
2025
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| Accès en ligne: | https://pubmed.ncbi.nlm.nih.gov/41597544/ |
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| _version_ | 1868266092685688833 |
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| author | Kurilenko, Valeriya Bystritskaya, Evgeniya Otstavnykh, Nadezhda Velansky, Peter Baldaev, Sergey Eremeev, Viacheslav Ageenko, Natalya Kiselev, Konstantin Nedashkovskaya, Olga Isaeva, Marina |
| author_facet | Kurilenko, Valeriya Bystritskaya, Evgeniya Otstavnykh, Nadezhda Velansky, Peter Baldaev, Sergey Eremeev, Viacheslav Ageenko, Natalya Kiselev, Konstantin Nedashkovskaya, Olga Isaeva, Marina Kurilenko, Valeriya Bystritskaya, Evgeniya Otstavnykh, Nadezhda Velansky, Peter Baldaev, Sergey Eremeev, Viacheslav Ageenko, Natalya Kiselev, Konstantin Nedashkovskaya, Olga Isaeva, Marina |
| collection | PubMed - marine biology |
| contents | Genome-Based Reclassification of [] Bowman and Nichols 2005 as gen. nov., comb. nov. and description of sp. nov. Kurilenko, Valeriya Bystritskaya, Evgeniya Otstavnykh, Nadezhda Velansky, Peter Baldaev, Sergey Eremeev, Viacheslav Ageenko, Natalya Kiselev, Konstantin Nedashkovskaya, Olga Isaeva, Marina The genus , a member of the phylum, is considered a polyphyletic taxonomic group requiring a phylogenetic revision of its members. A novel strain 041-53-Ur-6 was isolated from the cavity fluid of the sea urchin from the Sea of Okhotsk. Analysis of the 16S rRNA gene sequence showed that 041-53-Ur-6 belongs to the family , and its closest neighbor is [] with 97.5% sequence similarity. Phylogenomic analysis confirmed the phylogenetic heterogeneity of the genus and the clear separation of the genera "" and . The inter-genus AAI values between them were 74.0-76.4%, which is slightly lower than the inter-species AAI values observed for each genus. The strain 041-53-Ur-6 (= KMM 8389) formed a separated branch within the [] clade, demonstrating the highest ANI/AAI values of 80.1/81.0% with the strain [] APA-1. The dDDH values between strain KMM 8389 and representatives of the genus "" ranged from 22.6% to 26.7%. Major fatty acids were iso-C, iso-C and iso-C Δ2-OH. The polar lipids included a phosphatidylethanolamine, a phosphatidylglycerol, five unidentified lipids, two unidentified aminolipids, a phosphatidylcholine, and an unidentified aminophospholipid. The genome KMM 8389 is a circular chromosome of 3,031,910 bp in size with a DNA G + C content of 33.5%. It comprises 2702 protein-coding genes and four operons. Functional genomic analysis indicated the potential of KMM 8389 for degrading starch, glycogen, and alginate due to the presence of genes encoding GH13, GH31, and GH65. Furthermore, KMM 8389 possessed PLs 6, 7, 12, and 17, specialized for alginate, confirming the potential adaptation of this strain to algal substrates and surfaces. On the basis of the results of genotypic, chemotaxonomic, and phenotypic analyses, it is clear that the strain KMM 8389 represents a novel species with [.] , [] , [] , [] , [] , [] , and [] as the nearest neighbors. These taxa are classified in a single novel genus, as gen. nov., comb. nov., . comb. nov., . comb. nov., . comb. nov., . comb. nov., . comb. nov., . comb. nov., and sp. nov. 041-53-Ur-6 (=KMM 8389, =KCTC 72011). |
| format | Artículo científico |
| id | pubmed_41597544 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Microorganisms |
| record_format | pubmed |
| spellingShingle | Genome-Based Reclassification of [] Bowman and Nichols 2005 as gen. nov., comb. nov. and description of sp. nov. Kurilenko, Valeriya Bystritskaya, Evgeniya Otstavnykh, Nadezhda Velansky, Peter Baldaev, Sergey Eremeev, Viacheslav Ageenko, Natalya Kiselev, Konstantin Nedashkovskaya, Olga Isaeva, Marina Genome-Based Reclassification of [] Bowman and Nichols 2005 as gen. nov., comb. nov. and description of sp. nov. Kurilenko, Valeriya Bystritskaya, Evgeniya Otstavnykh, Nadezhda Velansky, Peter Baldaev, Sergey Eremeev, Viacheslav Ageenko, Natalya Kiselev, Konstantin Nedashkovskaya, Olga Isaeva, Marina The genus , a member of the phylum, is considered a polyphyletic taxonomic group requiring a phylogenetic revision of its members. A novel strain 041-53-Ur-6 was isolated from the cavity fluid of the sea urchin from the Sea of Okhotsk. Analysis of the 16S rRNA gene sequence showed that 041-53-Ur-6 belongs to the family , and its closest neighbor is [] with 97.5% sequence similarity. Phylogenomic analysis confirmed the phylogenetic heterogeneity of the genus and the clear separation of the genera "" and . The inter-genus AAI values between them were 74.0-76.4%, which is slightly lower than the inter-species AAI values observed for each genus. The strain 041-53-Ur-6 (= KMM 8389) formed a separated branch within the [] clade, demonstrating the highest ANI/AAI values of 80.1/81.0% with the strain [] APA-1. The dDDH values between strain KMM 8389 and representatives of the genus "" ranged from 22.6% to 26.7%. Major fatty acids were iso-C, iso-C and iso-C Δ2-OH. The polar lipids included a phosphatidylethanolamine, a phosphatidylglycerol, five unidentified lipids, two unidentified aminolipids, a phosphatidylcholine, and an unidentified aminophospholipid. The genome KMM 8389 is a circular chromosome of 3,031,910 bp in size with a DNA G + C content of 33.5%. It comprises 2702 protein-coding genes and four operons. Functional genomic analysis indicated the potential of KMM 8389 for degrading starch, glycogen, and alginate due to the presence of genes encoding GH13, GH31, and GH65. Furthermore, KMM 8389 possessed PLs 6, 7, 12, and 17, specialized for alginate, confirming the potential adaptation of this strain to algal substrates and surfaces. On the basis of the results of genotypic, chemotaxonomic, and phenotypic analyses, it is clear that the strain KMM 8389 represents a novel species with [.] , [] , [] , [] , [] , [] , and [] as the nearest neighbors. These taxa are classified in a single novel genus, as gen. nov., comb. nov., . comb. nov., . comb. nov., . comb. nov., . comb. nov., . comb. nov., . comb. nov., and sp. nov. 041-53-Ur-6 (=KMM 8389, =KCTC 72011). |
| title | Genome-Based Reclassification of [] Bowman and Nichols 2005 as gen. nov., comb. nov. and description of sp. nov. |
| url | https://pubmed.ncbi.nlm.nih.gov/41597544/ |