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| Main Authors: | , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Microorganisms
2026
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41597644/ |
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| _version_ | 1868266092685688832 |
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| author | Romanenko, Lyudmila Eremeev, Viacheslav Bystritskaya, Evgeniya Velansky, Peter Kurilenko, Valeriya Isaeva, Marina |
| author_facet | Romanenko, Lyudmila Eremeev, Viacheslav Bystritskaya, Evgeniya Velansky, Peter Kurilenko, Valeriya Isaeva, Marina Romanenko, Lyudmila Eremeev, Viacheslav Bystritskaya, Evgeniya Velansky, Peter Kurilenko, Valeriya Isaeva, Marina |
| collection | PubMed - marine biology |
| contents | Genomic Insights into sp. nov., Isolated from Okhotsk Sea Bottom Sediments, Suggest Plasmid-Mediated Strain-Specific Motility. Romanenko, Lyudmila Eremeev, Viacheslav Bystritskaya, Evgeniya Velansky, Peter Kurilenko, Valeriya Isaeva, Marina Two Gram-negative aerobic halophilic bacteria, designated KMM 9989 and KMM 9879, were isolated from a bottom sediment sample of the Okhotsk Sea, Russia. The novel strains grew in 0.5-4% NaCl, at 5-35 °C and pH 5.5-10.0. Phylogenetic analyses based on 16S rRNA gene and whole genome sequences placed strains KMM 9989 and KMM 9879 within the family , where they were clustered with their closest relative KCTC 22095. The average nucleotide identity (ANI) between strain KMM 9989 and KCTC 22095 was 81.4%. The level of digital DNA-DNA hybridization (dDDH) between the novel isolates KMM 9989 and KMM 9879 was 97%, while between strain KMM 9989 and KCTC 22095, it was 27%. Strains KMM 9989 and KMM 9879 contained Q-10 as the predominant ubiquinone and C as the major fatty acid. The polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, an unidentified aminolipid, two unidentified phospholipids, and three unidentified lipids. The genomic size of strains KMM 9989 and KMM 9879 was determined to be 4,040,543 bp and 3,969,839 bp with a DNA GC content of 61.3 and 61.4 mol%, respectively. Both strains contained a common plasmid of 238,277 bp and a strain-specific plasmid (188,734 bp for KMM 9989 and 118,029 bp for KMM 9879). It is suggested that the motility of KMM 9879 may be mediated by the presence of a complete -type operon in the strain-specific chromid. Thus, based on the phylogenetic analyses and distinctive phenotypic characteristics, the novel marine strains KMM 9989 and KMM 9879 are proposed to be classified as a novel species sp. nov. with the strain KMM 9989 (=KCTC 8835) as the type strain of the species. |
| format | Artículo científico |
| id | pubmed_41597644 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Microorganisms |
| record_format | pubmed |
| spellingShingle | Genomic Insights into sp. nov., Isolated from Okhotsk Sea Bottom Sediments, Suggest Plasmid-Mediated Strain-Specific Motility. Romanenko, Lyudmila Eremeev, Viacheslav Bystritskaya, Evgeniya Velansky, Peter Kurilenko, Valeriya Isaeva, Marina Genomic Insights into sp. nov., Isolated from Okhotsk Sea Bottom Sediments, Suggest Plasmid-Mediated Strain-Specific Motility. Romanenko, Lyudmila Eremeev, Viacheslav Bystritskaya, Evgeniya Velansky, Peter Kurilenko, Valeriya Isaeva, Marina Two Gram-negative aerobic halophilic bacteria, designated KMM 9989 and KMM 9879, were isolated from a bottom sediment sample of the Okhotsk Sea, Russia. The novel strains grew in 0.5-4% NaCl, at 5-35 °C and pH 5.5-10.0. Phylogenetic analyses based on 16S rRNA gene and whole genome sequences placed strains KMM 9989 and KMM 9879 within the family , where they were clustered with their closest relative KCTC 22095. The average nucleotide identity (ANI) between strain KMM 9989 and KCTC 22095 was 81.4%. The level of digital DNA-DNA hybridization (dDDH) between the novel isolates KMM 9989 and KMM 9879 was 97%, while between strain KMM 9989 and KCTC 22095, it was 27%. Strains KMM 9989 and KMM 9879 contained Q-10 as the predominant ubiquinone and C as the major fatty acid. The polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, an unidentified aminolipid, two unidentified phospholipids, and three unidentified lipids. The genomic size of strains KMM 9989 and KMM 9879 was determined to be 4,040,543 bp and 3,969,839 bp with a DNA GC content of 61.3 and 61.4 mol%, respectively. Both strains contained a common plasmid of 238,277 bp and a strain-specific plasmid (188,734 bp for KMM 9989 and 118,029 bp for KMM 9879). It is suggested that the motility of KMM 9879 may be mediated by the presence of a complete -type operon in the strain-specific chromid. Thus, based on the phylogenetic analyses and distinctive phenotypic characteristics, the novel marine strains KMM 9989 and KMM 9879 are proposed to be classified as a novel species sp. nov. with the strain KMM 9989 (=KCTC 8835) as the type strain of the species. |
| title | Genomic Insights into sp. nov., Isolated from Okhotsk Sea Bottom Sediments, Suggest Plasmid-Mediated Strain-Specific Motility. |
| url | https://pubmed.ncbi.nlm.nih.gov/41597644/ |