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| Main Authors: | , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Journal of applied microbiology
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41609416/ |
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Table of Contents:
- Genomic characterization of colistin- and carbapenem-resistant Pseudomonas aeruginosa ST1560 from Guanabara Bay, Brazil. Assunção, Vinicius Carneiro Magaldi, Mariana Lopes-Carvalho, Maiara Santos, Hugo Sérgio Oliveira Gonçalves-Brito, Andressa Vianna, Thereza Cristina Costa de Souza, Hosana Dau Ferreira Montenegro, Kaylanne Paranhos, Rodolfo Cardoso, Alexander Machado Bianco, Kayo Clementino, Maysa Mandetta Pseudomonas aeruginosa Colistin Brazil Carbapenems Anti-Bacterial Agents Microbial Sensitivity Tests beta-Lactamases Bacterial Proteins Genome, Bacterial Drug Resistance, Bacterial Drug Resistance, Multiple, Bacterial Water Microbiology This study aimed to characterize a colistin- and carbapenem-resistant Pseudomonas aeruginosa ST1560 strain isolated from Guanabara Bay, Brazil, and to investigate the molecular mechanisms underlying its resistance phenotype. Six surface water samples from Guanabara Bay were collected, yielding 71 P. aeruginosa subjected to antimicrobial susceptibility testing. Three isolates exhibited elevated minimal inhibitory concentrations (MICs) to colistin (≥512, 64, and 8 mg/l) in the absence of mcr genes (1-10). Among these, only strain CCVSU 5861 demonstrated carbapenemase confirmed by Blue Carba test. This strain was selected for whole-genome sequencing (Illumina). Genomic analysis identified the presence of blaKPC-2 and blaOXA-395, along with additional resistance determinants associated with aminoglycosides and fosfomycin. Genes involved in lipopolysaccharide modification, (arnA, arnT, and basS) were also detected, likely contributing to colistin resistance. The blaKPC-2 gene was located adjacent to the mobile genetic element ISKpn6, suggesting potential horizontal gene transfer. The P. aeruginosa ST1560 displays a complex multidrug resistance profile, including resistance to both colistin and carbapenems. This phenotype appears to be mediated by a combination of acquired resistance genes and chromosomal mechanisms. The localization of blaKPC-2 within a mobile genetic element underscores the risk of dissemination in aquatic environments.