Chitosan-collagen-G-90 film enhances wound closure and collagen remodeling: FTIR imaging and PCA-kNN analysis.
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| Format: | Artículo científico |
| Language: | en |
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Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
2026
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| _version_ | 1868266092668911616 |
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| author | Elmi, Fatemeh Elmi, Maryam Mitra Vaccari, Lisa Bedolla, Diana E |
| author_facet | Elmi, Fatemeh Elmi, Maryam Mitra Vaccari, Lisa Bedolla, Diana E Elmi, Fatemeh Elmi, Maryam Mitra Vaccari, Lisa Bedolla, Diana E |
| collection | PubMed - marine biology |
| contents | Chitosan-collagen-G-90 film enhances wound closure and collagen remodeling: FTIR imaging and PCA-kNN analysis. Elmi, Fatemeh Elmi, Maryam Mitra Vaccari, Lisa Bedolla, Diana E Chitosan Animals Collagen Spectroscopy, Fourier Transform Infrared Wound Healing Principal Component Analysis Rats Skin Chitosan-based films containing different bioactive components were developed to assess their effect on wound healing in a dermal rat skin tissue. Four formulations pure chitosan, chitosan-collagen (ACS), chitosan conjugated with glycolipoprotein extract from earth worm, namely chitosan-G-90, and a mixture of three constituents, chitosan-ACS-G-90 were compared with an untreated control. 14 days post-injury, wound closure was greatest in the chitosan-ACS-G-90 group. Ratio mapping (1325-1351 cm/1190-1357 cm) confirmed that the chitosan-ACS-G-90 film provided the most consistent improvement in collagen organization, highlighting its superior role in matrix maturation. Vector-normalized, second-derivative spectra from the protein (1710-1475 cm) and fingerprint (1800-900 cm) regions revealed treatment-dependent variations in amide I/II profiles, collagen triple-helix markers (1338 cm, 1203 cm), and distinct absorptions in the carbohydrate associated region (∼1150-1000 cm). These bands while often attributed to glycosaminoglycans or other ECM polysaccharides may also include overlapping contributions from collagen side chains and nucleic acid phosphate vibrations (DNA/RNA), reflecting the inherently complex biochemical composition of dermal ECM. Principal Component Analysis (PCA) clearly separated the chitosan-ACS-G-90 group from untreated dermis, with loadings associated with cross-link-related (∼1690 cm) and amide I/II bands (∼1658 cm, 1566-1543 cm). Classification of PCA scores using k-Nearest Neighbors (kNN) achieved high accuracy (AUC = 0.993). These results show that the chitosan-ACS-G-90 accelerates wound closure and enhances collagen remodeling. |
| format | Artículo científico |
| id | pubmed_41610598 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy |
| record_format | pubmed |
| spellingShingle | Chitosan-collagen-G-90 film enhances wound closure and collagen remodeling: FTIR imaging and PCA-kNN analysis. Elmi, Fatemeh Elmi, Maryam Mitra Vaccari, Lisa Bedolla, Diana E Chitosan Animals Collagen Spectroscopy, Fourier Transform Infrared Wound Healing Principal Component Analysis Rats Skin Chitosan-collagen-G-90 film enhances wound closure and collagen remodeling: FTIR imaging and PCA-kNN analysis. Elmi, Fatemeh Elmi, Maryam Mitra Vaccari, Lisa Bedolla, Diana E Chitosan Animals Collagen Spectroscopy, Fourier Transform Infrared Wound Healing Principal Component Analysis Rats Skin Chitosan-based films containing different bioactive components were developed to assess their effect on wound healing in a dermal rat skin tissue. Four formulations pure chitosan, chitosan-collagen (ACS), chitosan conjugated with glycolipoprotein extract from earth worm, namely chitosan-G-90, and a mixture of three constituents, chitosan-ACS-G-90 were compared with an untreated control. 14 days post-injury, wound closure was greatest in the chitosan-ACS-G-90 group. Ratio mapping (1325-1351 cm/1190-1357 cm) confirmed that the chitosan-ACS-G-90 film provided the most consistent improvement in collagen organization, highlighting its superior role in matrix maturation. Vector-normalized, second-derivative spectra from the protein (1710-1475 cm) and fingerprint (1800-900 cm) regions revealed treatment-dependent variations in amide I/II profiles, collagen triple-helix markers (1338 cm, 1203 cm), and distinct absorptions in the carbohydrate associated region (∼1150-1000 cm). These bands while often attributed to glycosaminoglycans or other ECM polysaccharides may also include overlapping contributions from collagen side chains and nucleic acid phosphate vibrations (DNA/RNA), reflecting the inherently complex biochemical composition of dermal ECM. Principal Component Analysis (PCA) clearly separated the chitosan-ACS-G-90 group from untreated dermis, with loadings associated with cross-link-related (∼1690 cm) and amide I/II bands (∼1658 cm, 1566-1543 cm). Classification of PCA scores using k-Nearest Neighbors (kNN) achieved high accuracy (AUC = 0.993). These results show that the chitosan-ACS-G-90 accelerates wound closure and enhances collagen remodeling. |
| title | Chitosan-collagen-G-90 film enhances wound closure and collagen remodeling: FTIR imaging and PCA-kNN analysis. |
| topic | Chitosan Animals Collagen Spectroscopy, Fourier Transform Infrared Wound Healing Principal Component Analysis Rats Skin |
| url | https://pubmed.ncbi.nlm.nih.gov/41610598/ |