Isolation and Cultivation of Diplonemids.
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PubMed
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| Auteurs principaux: | , , , , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
| Publié: |
Methods in molecular biology (Clifton, N.J.)
2026
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| _version_ | 1868266092648988673 |
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| author | Tashyreva, Daria Prokopchuk, Galina Iorillo, Orsola Valach, Matus Škodová-Sveráková, Ingrid Shin, Dong-Woo Letts, James A Burger, Gertraud Lukeš, Julius Faktorová, Drahomíra |
| author_facet | Tashyreva, Daria Prokopchuk, Galina Iorillo, Orsola Valach, Matus Škodová-Sveráková, Ingrid Shin, Dong-Woo Letts, James A Burger, Gertraud Lukeš, Julius Faktorová, Drahomíra Tashyreva, Daria Prokopchuk, Galina Iorillo, Orsola Valach, Matus Škodová-Sveráková, Ingrid Shin, Dong-Woo Letts, James A Burger, Gertraud Lukeš, Julius Faktorová, Drahomíra |
| collection | PubMed - marine biology |
| contents | Isolation and Cultivation of Diplonemids. Tashyreva, Daria Prokopchuk, Galina Iorillo, Orsola Valach, Matus Škodová-Sveráková, Ingrid Shin, Dong-Woo Letts, James A Burger, Gertraud Lukeš, Julius Faktorová, Drahomíra Cryopreservation Animals Axenic Culture Cell Culture Techniques Marine diplonemids (Diplonemidae and Hemistasiidae), once considered difficult to culture, can now be introduced into stable axenic cultures and grown to high densities. This chapter provides detailed protocols on establishing clonal cultures from environmental cells, their axenization and routine maintenance, as well as instructions on mid- and large-scale cultivation, estimation of cell density, and cryopreservation of selected species. |
| format | Artículo científico |
| id | pubmed_41627728 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Methods in molecular biology (Clifton, N.J.) |
| record_format | pubmed |
| spellingShingle | Isolation and Cultivation of Diplonemids. Tashyreva, Daria Prokopchuk, Galina Iorillo, Orsola Valach, Matus Škodová-Sveráková, Ingrid Shin, Dong-Woo Letts, James A Burger, Gertraud Lukeš, Julius Faktorová, Drahomíra Cryopreservation Animals Axenic Culture Cell Culture Techniques Isolation and Cultivation of Diplonemids. Tashyreva, Daria Prokopchuk, Galina Iorillo, Orsola Valach, Matus Škodová-Sveráková, Ingrid Shin, Dong-Woo Letts, James A Burger, Gertraud Lukeš, Julius Faktorová, Drahomíra Cryopreservation Animals Axenic Culture Cell Culture Techniques Marine diplonemids (Diplonemidae and Hemistasiidae), once considered difficult to culture, can now be introduced into stable axenic cultures and grown to high densities. This chapter provides detailed protocols on establishing clonal cultures from environmental cells, their axenization and routine maintenance, as well as instructions on mid- and large-scale cultivation, estimation of cell density, and cryopreservation of selected species. |
| title | Isolation and Cultivation of Diplonemids. |
| topic | Cryopreservation Animals Axenic Culture Cell Culture Techniques |
| url | https://pubmed.ncbi.nlm.nih.gov/41627728/ |