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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Methods in molecular biology (Clifton, N.J.)
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41627728/ |
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Table of Contents:
- Isolation and Cultivation of Diplonemids. Tashyreva, Daria Prokopchuk, Galina Iorillo, Orsola Valach, Matus Škodová-Sveráková, Ingrid Shin, Dong-Woo Letts, James A Burger, Gertraud Lukeš, Julius Faktorová, Drahomíra Cryopreservation Animals Axenic Culture Cell Culture Techniques Marine diplonemids (Diplonemidae and Hemistasiidae), once considered difficult to culture, can now be introduced into stable axenic cultures and grown to high densities. This chapter provides detailed protocols on establishing clonal cultures from environmental cells, their axenization and routine maintenance, as well as instructions on mid- and large-scale cultivation, estimation of cell density, and cryopreservation of selected species.