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| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Nature communications
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41629293/ |
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| _version_ | 1868266090068443136 |
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| author | Huang, Zhengyun Liu, Xiangpeng Chen, Xiyue Zhou, You Chen, Qian Liu, Yan Zhu, Hongyun Cheng, Ken Feng, Yu Dong, Miren Song, Linsheng Wang, Lingling Liu, Shiqi Shan, Tizhong Kuang, Shihuan Dong, Yingying Vidal-Puig, Antonio Zhang, Yong Jia, Zhihao |
| author_facet | Huang, Zhengyun Liu, Xiangpeng Chen, Xiyue Zhou, You Chen, Qian Liu, Yan Zhu, Hongyun Cheng, Ken Feng, Yu Dong, Miren Song, Linsheng Wang, Lingling Liu, Shiqi Shan, Tizhong Kuang, Shihuan Dong, Yingying Vidal-Puig, Antonio Zhang, Yong Jia, Zhihao Huang, Zhengyun Liu, Xiangpeng Chen, Xiyue Zhou, You Chen, Qian Liu, Yan Zhu, Hongyun Cheng, Ken Feng, Yu Dong, Miren Song, Linsheng Wang, Lingling Liu, Shiqi Shan, Tizhong Kuang, Shihuan Dong, Yingying Vidal-Puig, Antonio Zhang, Yong Jia, Zhihao |
| collection | PubMed - marine biology |
| contents | PRMT3-mediated post-translational adaptation to fasting regulates metabolic flexibility. Huang, Zhengyun Liu, Xiangpeng Chen, Xiyue Zhou, You Chen, Qian Liu, Yan Zhu, Hongyun Cheng, Ken Feng, Yu Dong, Miren Song, Linsheng Wang, Lingling Liu, Shiqi Shan, Tizhong Kuang, Shihuan Dong, Yingying Vidal-Puig, Antonio Zhang, Yong Jia, Zhihao Animals Protein-Arginine N-Methyltransferases Male Mice Arginine Fasting Obesity Intermittent Fasting Insulin Mice, Inbred C57BL Methylation Adipocytes Protein Processing, Post-Translational Insulin Resistance Signal Transduction Humans Mice, Knockout Adaptation, Physiological Diet, High-Fat Obesity impairs metabolic flexibility-the capacity to adapt to fluctuating energy demands. Emerging evidence suggests that dietary interventions, particularly time-restricted feeding (TRF), may help restore this flexibility. In this study, we demonstrate that feeding upregulates PRMT3 and asymmetric dimethylarginine (ADMA)-containing proteins via insulin-pAKT signaling, while fasting reduces their expression. Pharmacological inhibition of PRMT3 attenuates diet-induced obesity (DIO) and enhances adipocyte glycolysis in male mice. Mechanistically, PRMT3 drives the expression of citrate transporter SLC25A1 during feeding through direct arginine methylation. A 16:8 TRF regimen normalizes PRMT3 and ADMA levels while suppressing SLC25A1 expression. Notably, PRMT3 inhibition recapitulates the metabolic benefits of 16:8 TRF and improves metabolic flexibility. Furthermore, adipocyte-specific deletion of Slc25a1 in male mice protects against DIO and enhances insulin sensitivity. Collectively, these findings identify PRMT3-mediated arginine methylation in vWAT as a nutrient-responsive regulatory axis that impairs metabolic flexibility in obesity, which is a potential therapeutic target. |
| format | Artículo científico |
| id | pubmed_41629293 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Nature communications |
| record_format | pubmed |
| spellingShingle | PRMT3-mediated post-translational adaptation to fasting regulates metabolic flexibility. Huang, Zhengyun Liu, Xiangpeng Chen, Xiyue Zhou, You Chen, Qian Liu, Yan Zhu, Hongyun Cheng, Ken Feng, Yu Dong, Miren Song, Linsheng Wang, Lingling Liu, Shiqi Shan, Tizhong Kuang, Shihuan Dong, Yingying Vidal-Puig, Antonio Zhang, Yong Jia, Zhihao Animals Protein-Arginine N-Methyltransferases Male Mice Arginine Fasting Obesity Intermittent Fasting Insulin Mice, Inbred C57BL Methylation Adipocytes Protein Processing, Post-Translational Insulin Resistance Signal Transduction Humans Mice, Knockout Adaptation, Physiological Diet, High-Fat PRMT3-mediated post-translational adaptation to fasting regulates metabolic flexibility. Huang, Zhengyun Liu, Xiangpeng Chen, Xiyue Zhou, You Chen, Qian Liu, Yan Zhu, Hongyun Cheng, Ken Feng, Yu Dong, Miren Song, Linsheng Wang, Lingling Liu, Shiqi Shan, Tizhong Kuang, Shihuan Dong, Yingying Vidal-Puig, Antonio Zhang, Yong Jia, Zhihao Animals Protein-Arginine N-Methyltransferases Male Mice Arginine Fasting Obesity Intermittent Fasting Insulin Mice, Inbred C57BL Methylation Adipocytes Protein Processing, Post-Translational Insulin Resistance Signal Transduction Humans Mice, Knockout Adaptation, Physiological Diet, High-Fat Obesity impairs metabolic flexibility-the capacity to adapt to fluctuating energy demands. Emerging evidence suggests that dietary interventions, particularly time-restricted feeding (TRF), may help restore this flexibility. In this study, we demonstrate that feeding upregulates PRMT3 and asymmetric dimethylarginine (ADMA)-containing proteins via insulin-pAKT signaling, while fasting reduces their expression. Pharmacological inhibition of PRMT3 attenuates diet-induced obesity (DIO) and enhances adipocyte glycolysis in male mice. Mechanistically, PRMT3 drives the expression of citrate transporter SLC25A1 during feeding through direct arginine methylation. A 16:8 TRF regimen normalizes PRMT3 and ADMA levels while suppressing SLC25A1 expression. Notably, PRMT3 inhibition recapitulates the metabolic benefits of 16:8 TRF and improves metabolic flexibility. Furthermore, adipocyte-specific deletion of Slc25a1 in male mice protects against DIO and enhances insulin sensitivity. Collectively, these findings identify PRMT3-mediated arginine methylation in vWAT as a nutrient-responsive regulatory axis that impairs metabolic flexibility in obesity, which is a potential therapeutic target. |
| title | PRMT3-mediated post-translational adaptation to fasting regulates metabolic flexibility. |
| topic | Animals Protein-Arginine N-Methyltransferases Male Mice Arginine Fasting Obesity Intermittent Fasting Insulin Mice, Inbred C57BL Methylation Adipocytes Protein Processing, Post-Translational Insulin Resistance Signal Transduction Humans Mice, Knockout Adaptation, Physiological Diet, High-Fat |
| url | https://pubmed.ncbi.nlm.nih.gov/41629293/ |