Designing a Hypoallergenic Mutant of Cra a 2 with T-Cell Reactivity.
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| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
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Journal of agricultural and food chemistry
2026
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| _version_ | 1868266090060054529 |
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| author | Huan, Fei Gao, Shuai Gu, Yi Li, Yong-Peng Huang, Xin-Yi Liu, Meng Yang, Yang Lai, Dong Xiao, An-Feng Liu, Guang-Ming |
| author_facet | Huan, Fei Gao, Shuai Gu, Yi Li, Yong-Peng Huang, Xin-Yi Liu, Meng Yang, Yang Lai, Dong Xiao, An-Feng Liu, Guang-Ming Huan, Fei Gao, Shuai Gu, Yi Li, Yong-Peng Huang, Xin-Yi Liu, Meng Yang, Yang Lai, Dong Xiao, An-Feng Liu, Guang-Ming |
| collection | PubMed - marine biology |
| contents | Designing a Hypoallergenic Mutant of Cra a 2 with T-Cell Reactivity. Huan, Fei Gao, Shuai Gu, Yi Li, Yong-Peng Huang, Xin-Yi Liu, Meng Yang, Yang Lai, Dong Xiao, An-Feng Liu, Guang-Ming Animals T-Lymphocytes Mice Humans Ostreidae Allergens Arginine Kinase Immunoglobulin E Female Mice, Inbred BALB C Immunoglobulin G Epitopes, T-Lymphocyte Mutation Shellfish Hypersensitivity Crossostrea angulata arginine kinase (Cra a 2) is a major allergen responsible for oyster allergy, emphasizing the need to develop its hypoallergenic mutant for oral desensitization. In this study, a mutant Cra a 2 (mCra a 2) was generated by simultaneously substituting the four key amino acids within the conformational B-cell epitopes. These site-directed mutations altered the structure and surface electrostatic potential, leading to reduction in immunoglobulin (Ig)G-/IgE-binding capacity and attenuated basophil activation, thereby confirming the diminished allergenic potential of mCra a 2. For T-cell reactivity analysis, two T-cell epitopes were identified and found to remain preserved in mCra a 2, thereby preserving its ability to activate T-cell. Notably, splenocytes from wild-type Cra a 2-immunized mice stimulation with mCra a 2 up-regulate IL-10 secretion. Collectively, mCra a 2 not only exhibits attenuated immunoreactivity but also retains robust T-cell reactivity, suggesting its potential utility in oral desensitization of oyster allergy. |
| format | Artículo científico |
| id | pubmed_41632806 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Journal of agricultural and food chemistry |
| record_format | pubmed |
| spellingShingle | Designing a Hypoallergenic Mutant of Cra a 2 with T-Cell Reactivity. Huan, Fei Gao, Shuai Gu, Yi Li, Yong-Peng Huang, Xin-Yi Liu, Meng Yang, Yang Lai, Dong Xiao, An-Feng Liu, Guang-Ming Animals T-Lymphocytes Mice Humans Ostreidae Allergens Arginine Kinase Immunoglobulin E Female Mice, Inbred BALB C Immunoglobulin G Epitopes, T-Lymphocyte Mutation Shellfish Hypersensitivity Designing a Hypoallergenic Mutant of Cra a 2 with T-Cell Reactivity. Huan, Fei Gao, Shuai Gu, Yi Li, Yong-Peng Huang, Xin-Yi Liu, Meng Yang, Yang Lai, Dong Xiao, An-Feng Liu, Guang-Ming Animals T-Lymphocytes Mice Humans Ostreidae Allergens Arginine Kinase Immunoglobulin E Female Mice, Inbred BALB C Immunoglobulin G Epitopes, T-Lymphocyte Mutation Shellfish Hypersensitivity Crossostrea angulata arginine kinase (Cra a 2) is a major allergen responsible for oyster allergy, emphasizing the need to develop its hypoallergenic mutant for oral desensitization. In this study, a mutant Cra a 2 (mCra a 2) was generated by simultaneously substituting the four key amino acids within the conformational B-cell epitopes. These site-directed mutations altered the structure and surface electrostatic potential, leading to reduction in immunoglobulin (Ig)G-/IgE-binding capacity and attenuated basophil activation, thereby confirming the diminished allergenic potential of mCra a 2. For T-cell reactivity analysis, two T-cell epitopes were identified and found to remain preserved in mCra a 2, thereby preserving its ability to activate T-cell. Notably, splenocytes from wild-type Cra a 2-immunized mice stimulation with mCra a 2 up-regulate IL-10 secretion. Collectively, mCra a 2 not only exhibits attenuated immunoreactivity but also retains robust T-cell reactivity, suggesting its potential utility in oral desensitization of oyster allergy. |
| title | Designing a Hypoallergenic Mutant of Cra a 2 with T-Cell Reactivity. |
| topic | Animals T-Lymphocytes Mice Humans Ostreidae Allergens Arginine Kinase Immunoglobulin E Female Mice, Inbred BALB C Immunoglobulin G Epitopes, T-Lymphocyte Mutation Shellfish Hypersensitivity |
| url | https://pubmed.ncbi.nlm.nih.gov/41632806/ |