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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Journal of agricultural and food chemistry
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41632806/ |
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Table of Contents:
- Designing a Hypoallergenic Mutant of Cra a 2 with T-Cell Reactivity. Huan, Fei Gao, Shuai Gu, Yi Li, Yong-Peng Huang, Xin-Yi Liu, Meng Yang, Yang Lai, Dong Xiao, An-Feng Liu, Guang-Ming Animals T-Lymphocytes Mice Humans Ostreidae Allergens Arginine Kinase Immunoglobulin E Female Mice, Inbred BALB C Immunoglobulin G Epitopes, T-Lymphocyte Mutation Shellfish Hypersensitivity Crossostrea angulata arginine kinase (Cra a 2) is a major allergen responsible for oyster allergy, emphasizing the need to develop its hypoallergenic mutant for oral desensitization. In this study, a mutant Cra a 2 (mCra a 2) was generated by simultaneously substituting the four key amino acids within the conformational B-cell epitopes. These site-directed mutations altered the structure and surface electrostatic potential, leading to reduction in immunoglobulin (Ig)G-/IgE-binding capacity and attenuated basophil activation, thereby confirming the diminished allergenic potential of mCra a 2. For T-cell reactivity analysis, two T-cell epitopes were identified and found to remain preserved in mCra a 2, thereby preserving its ability to activate T-cell. Notably, splenocytes from wild-type Cra a 2-immunized mice stimulation with mCra a 2 up-regulate IL-10 secretion. Collectively, mCra a 2 not only exhibits attenuated immunoreactivity but also retains robust T-cell reactivity, suggesting its potential utility in oral desensitization of oyster allergy.