A visual protocol for sensitive detection based on hybridization chain reaction cascaded DNAzyme.
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| Auteurs principaux: | , , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
| Publié: |
Analytical methods : advancing methods and applications
2026
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| _version_ | 1868266090050617344 |
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| author | Wang, Jiali Cui, Qian Zeng, Shu Fu, Danfeng Peng, Daoyun Chen, Fei Wan, Yi Wang, Anwei |
| author_facet | Wang, Jiali Cui, Qian Zeng, Shu Fu, Danfeng Peng, Daoyun Chen, Fei Wan, Yi Wang, Anwei Wang, Jiali Cui, Qian Zeng, Shu Fu, Danfeng Peng, Daoyun Chen, Fei Wan, Yi Wang, Anwei |
| collection | PubMed - marine biology |
| contents | A visual protocol for sensitive detection based on hybridization chain reaction cascaded DNAzyme. Wang, Jiali Cui, Qian Zeng, Shu Fu, Danfeng Peng, Daoyun Chen, Fei Wan, Yi Wang, Anwei Vibrio parahaemolyticus DNA, Catalytic Nucleic Acid Hybridization Aptamers, Nucleotide Limit of Detection () is one of the most harmful pathogenic bacteria derived from seafood, and it can cause great damage to aquaculture and human health, even at low concentrations. The rapid and accurate detection of in clinical samples is essential but remains a challenge. Herein, we have developed a highly sensitive, simple and visual method for detection. The capture probe is composed of a -specific aptamer, with its blocking strands designated as the initiator (I), and magnetic beads (MB). In the presence of , the aptamer on the probe specifically recognizes and binds to , leading to the separation of the I strand. Next, the I strand sequentially initiates a hybridization chain reaction (HCR) for signal amplification, releasing the G-quadruplex, which was blocked in hairpin H2, and recovers its peroxidase-mimicking activity. Thus, the results can be visualized through the color variation of 2,2'-azino-di-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), which displays green for -containing samples and colorless for negative samples. With the signal amplification of HCR, this method can achieve a detection limit of 0.82 CFU mL with a wide linear detection range from 10 to 10 CFU mL. It also exhibited excellent specificity against common non-target bacteria in aquaculture and performed well in the detection of -spiked Pacific white shrimp. Furthermore, this method realized the accurate detection of in shrimp seedlings from six different breeding bases in Wenchang, Hainan Province, which indicates tremendous potential for early disease diagnosis and prevention. |
| format | Artículo científico |
| id | pubmed_41636755 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Analytical methods : advancing methods and applications |
| record_format | pubmed |
| spellingShingle | A visual protocol for sensitive detection based on hybridization chain reaction cascaded DNAzyme. Wang, Jiali Cui, Qian Zeng, Shu Fu, Danfeng Peng, Daoyun Chen, Fei Wan, Yi Wang, Anwei Vibrio parahaemolyticus DNA, Catalytic Nucleic Acid Hybridization Aptamers, Nucleotide Limit of Detection A visual protocol for sensitive detection based on hybridization chain reaction cascaded DNAzyme. Wang, Jiali Cui, Qian Zeng, Shu Fu, Danfeng Peng, Daoyun Chen, Fei Wan, Yi Wang, Anwei Vibrio parahaemolyticus DNA, Catalytic Nucleic Acid Hybridization Aptamers, Nucleotide Limit of Detection () is one of the most harmful pathogenic bacteria derived from seafood, and it can cause great damage to aquaculture and human health, even at low concentrations. The rapid and accurate detection of in clinical samples is essential but remains a challenge. Herein, we have developed a highly sensitive, simple and visual method for detection. The capture probe is composed of a -specific aptamer, with its blocking strands designated as the initiator (I), and magnetic beads (MB). In the presence of , the aptamer on the probe specifically recognizes and binds to , leading to the separation of the I strand. Next, the I strand sequentially initiates a hybridization chain reaction (HCR) for signal amplification, releasing the G-quadruplex, which was blocked in hairpin H2, and recovers its peroxidase-mimicking activity. Thus, the results can be visualized through the color variation of 2,2'-azino-di-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), which displays green for -containing samples and colorless for negative samples. With the signal amplification of HCR, this method can achieve a detection limit of 0.82 CFU mL with a wide linear detection range from 10 to 10 CFU mL. It also exhibited excellent specificity against common non-target bacteria in aquaculture and performed well in the detection of -spiked Pacific white shrimp. Furthermore, this method realized the accurate detection of in shrimp seedlings from six different breeding bases in Wenchang, Hainan Province, which indicates tremendous potential for early disease diagnosis and prevention. |
| title | A visual protocol for sensitive detection based on hybridization chain reaction cascaded DNAzyme. |
| topic | Vibrio parahaemolyticus DNA, Catalytic Nucleic Acid Hybridization Aptamers, Nucleotide Limit of Detection |
| url | https://pubmed.ncbi.nlm.nih.gov/41636755/ |