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| Main Authors: | , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
eLife
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41642075/ |
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| _version_ | 1868266090043277312 |
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| author | Yamamoto, Sakiya Furukawa, Saya Ohashi, Ayaka Hamada, Mayuko Satoh, Akira |
| author_facet | Yamamoto, Sakiya Furukawa, Saya Ohashi, Ayaka Hamada, Mayuko Satoh, Akira Yamamoto, Sakiya Furukawa, Saya Ohashi, Ayaka Hamada, Mayuko Satoh, Akira |
| collection | PubMed - marine biology |
| contents | Dorsoventral-mediated induction is required for axolotl limb regeneration. Yamamoto, Sakiya Furukawa, Saya Ohashi, Ayaka Hamada, Mayuko Satoh, Akira Animals Hedgehog Proteins Regeneration Ambystoma mexicanum Extremities Body Patterning Gene Expression Regulation, Developmental Signal Transduction Axolotls () exhibit a remarkable ability to regenerate limbs. Classical experiments have suggested that contact between cells derived from distinct orientations-dorsal, ventral, anterior, and posterior-within the regenerating blastema is necessary for accurate limb pattern formation. However, the molecular basis for this requirement has remained largely unknown. Here, we demonstrate that both dorsal and ventral tissues are required for limb formation via induction of expression, which plays a crucial role in limb patterning. Using the accessory limb model, we induced position-specific blastemas lacking cells derived from a single orientation (anterior, posterior, dorsal, or ventral). Limb patterning occurred only in blastemas containing both dorsal- and ventral-derived cells. We further observed that expression requires dorsoventral contact within a blastema, highlighting the necessity of dorsoventral contact for inducing expression. Additionally, we identified WNT10B and FGF2 as dorsal- and ventral-mediated signals, respectively, that create the inductive environment for expression. Our findings clarify the role of dorsal and ventral cells in inducing , a mechanism that has rarely been studied in the context of limb regeneration and pattern formation. This model provides new insights into how cells with different positional identities drive the regeneration process. |
| format | Artículo científico |
| id | pubmed_41642075 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | eLife |
| record_format | pubmed |
| spellingShingle | Dorsoventral-mediated induction is required for axolotl limb regeneration. Yamamoto, Sakiya Furukawa, Saya Ohashi, Ayaka Hamada, Mayuko Satoh, Akira Animals Hedgehog Proteins Regeneration Ambystoma mexicanum Extremities Body Patterning Gene Expression Regulation, Developmental Signal Transduction Dorsoventral-mediated induction is required for axolotl limb regeneration. Yamamoto, Sakiya Furukawa, Saya Ohashi, Ayaka Hamada, Mayuko Satoh, Akira Animals Hedgehog Proteins Regeneration Ambystoma mexicanum Extremities Body Patterning Gene Expression Regulation, Developmental Signal Transduction Axolotls () exhibit a remarkable ability to regenerate limbs. Classical experiments have suggested that contact between cells derived from distinct orientations-dorsal, ventral, anterior, and posterior-within the regenerating blastema is necessary for accurate limb pattern formation. However, the molecular basis for this requirement has remained largely unknown. Here, we demonstrate that both dorsal and ventral tissues are required for limb formation via induction of expression, which plays a crucial role in limb patterning. Using the accessory limb model, we induced position-specific blastemas lacking cells derived from a single orientation (anterior, posterior, dorsal, or ventral). Limb patterning occurred only in blastemas containing both dorsal- and ventral-derived cells. We further observed that expression requires dorsoventral contact within a blastema, highlighting the necessity of dorsoventral contact for inducing expression. Additionally, we identified WNT10B and FGF2 as dorsal- and ventral-mediated signals, respectively, that create the inductive environment for expression. Our findings clarify the role of dorsal and ventral cells in inducing , a mechanism that has rarely been studied in the context of limb regeneration and pattern formation. This model provides new insights into how cells with different positional identities drive the regeneration process. |
| title | Dorsoventral-mediated induction is required for axolotl limb regeneration. |
| topic | Animals Hedgehog Proteins Regeneration Ambystoma mexicanum Extremities Body Patterning Gene Expression Regulation, Developmental Signal Transduction |
| url | https://pubmed.ncbi.nlm.nih.gov/41642075/ |