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Bibliographic Details
Main Authors: Calhau, Isabel B, Gomes, Ana C, Bruno, Sofia M, Rosário, Fernanda, Oliveira, Laura, Pereira, Carla, Oliveira, Helena, Almeida, Adelaide, Gonçalves, Isabel S, Pillinger, Martyn
Format: Artículo científico
Language:en
Published: Journal of inorganic biochemistry 2026
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Online Access:https://pubmed.ncbi.nlm.nih.gov/41643237/
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Table of Contents:
  • Molybdenum(II) allyl dicarbonyl complexes with 3-(2-pyridyl)pyrazole as potential CO-releasing molecules, antibacterial and antitumoral agents. Calhau, Isabel B Gomes, Ana C Bruno, Sofia M Rosário, Fernanda Oliveira, Laura Pereira, Carla Oliveira, Helena Almeida, Adelaide Gonçalves, Isabel S Pillinger, Martyn Antineoplastic Agents Humans Anti-Bacterial Agents Molybdenum Escherichia coli Coordination Complexes Pyrazoles Carbon Monoxide Cell Line, Tumor Methicillin-Resistant Staphylococcus aureus Microbial Sensitivity Tests Molybdenum-based CO-releasing molecules (CORMs) are attracting interest for biological and therapeutic applications. In this work two new complexes, [Mo(η-CH)X(CO)(Hpypz)] [X = Cl (1), Br (2); Hpypz = 3-(2-pyridyl)pyrazole] have been synthesized, characterized, and evaluated for their CO-release capacity, as well as antimicrobial and anticancer potential. The evaluation of the CO-release properties by the deoxymyoglobin‑carbonmonoxymyoglobin assay showed that the two complexes are comparably slow CO releasers in aqueous systems, showing a half-life of several hours, and sustained CO release over the course of the assay (6 h). Studies of biological activity were performed with complex 1 due to its better aqueous solubility. The antibacterial activity was investigated by determination of the minimum inhibitory and minimum bactericidal concentrations with the microdilution assay for gram-positive methicillin-resistant Staphylococcus aureus (MRSA) and gram-negative Escherichia coli strains. Complex 1 displayed appreciable concentration-dependent bactericidal activity against both strains. The cytotoxicity of complex 1 was evaluated on human melanoma cells (A375) and immortalized nontumorigenic keratinocytes (HaCaT). Complex 1 exhibited selective cytotoxicity, significantly reducing the cell viability of A375 cells in a dose-dependent manner while having a lower effect on HaCaT cells, suggesting its antitumor potential against melanoma. In contrast, the precursor complex [Mo(η-CH)Cl(CO)(CHCN)] showed reduced activity against A375 cells and higher toxicity toward HaCaT cells, highlighting the beneficial impact of the bidentate 3-(2-pyridyl)pyrazole ligand.