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| Format: | Artículo científico |
| Language: | en |
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Acta parasitologica
2026
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41661452/ |
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| author | Naguib, Doaa Alhussainy, Nabeel H El-Wahsh, Hany M Al Braikan, Faten A Basalem, Osama Salem Mal, Walaa Abdulsamad Ahmed, Ashraf Fawzy Mosa Baakdah, Fadi Alshumrani, Mohammad Mousa Darwish, Wael Sobhy Bakhsh, Wesam Kamal |
| author_facet | Naguib, Doaa Alhussainy, Nabeel H El-Wahsh, Hany M Al Braikan, Faten A Basalem, Osama Salem Mal, Walaa Abdulsamad Ahmed, Ashraf Fawzy Mosa Baakdah, Fadi Alshumrani, Mohammad Mousa Darwish, Wael Sobhy Bakhsh, Wesam Kamal Naguib, Doaa Alhussainy, Nabeel H El-Wahsh, Hany M Al Braikan, Faten A Basalem, Osama Salem Mal, Walaa Abdulsamad Ahmed, Ashraf Fawzy Mosa Baakdah, Fadi Alshumrani, Mohammad Mousa Darwish, Wael Sobhy Bakhsh, Wesam Kamal |
| collection | PubMed - marine biology |
| contents | Prevalence and Subtypes of Blastocystis in Symptomatic Children in Egypt and Anti-Blastocystis Efficacy of Artemisia alba-Loaded Silver Nanoparticles In Vitro and in Swiss Albino Mice. Naguib, Doaa Alhussainy, Nabeel H El-Wahsh, Hany M Al Braikan, Faten A Basalem, Osama Salem Mal, Walaa Abdulsamad Ahmed, Ashraf Fawzy Mosa Baakdah, Fadi Alshumrani, Mohammad Mousa Darwish, Wael Sobhy Bakhsh, Wesam Kamal PURPOSE: Blastocystis hominis (B. hominis) is a common protozoan pathogen that affects the health of both humans and animals. This study aimed to investigate the prevalence of Blastocystis in children and evaluate the effects of Artemisia alba aqueous extract-loaded silver nanoparticles (AgNPs) on B. hominis infection, both in vitro and in mice. METHODS: Fresh fecal samples were collected from 235 children and analyzed for the presence of B. hominis using microscopy and culture techniques. Fifty positive samples were further subtyped using subtype-specific primers through PCR. Additionally, the effectiveness of Artemisia alba aqueous extract-loaded AgNPs in treating B. hominis infection was assessed both in vitro and in Swiss albino mice. RESULTS: Of the 235 children's fecal samples, 75 (32%) tested positive for B. hominis. Genotyping of 50 positive samples revealed that ST3 was the most common genotype, followed by ST1, ST2, and ST4. The results of the second phase showed that treatment with Artemisia alba aqueous extract-loaded AgNPs significantly inhibited the growth of Blastocystis in culture. Additionally, it significantly reduced the number of Blastocystis cysts found in the feces of treated mice. Furthermore, this treatment had minimal adverse effects on the liver, kidney, and intestine, especially when compared to metronidazole. CONCLUSION: Blastocystis is commonly found among children, with subtype ST3 being the most prevalent. The administration of Artemisia alba aqueous extract-loaded AgNPs demonstrated significant anti-Blastocystis activity, both in vitro and in vivo. Furthermore, the treatment was shown to be safe and may represent a promising option for addressing blastocystosis. |
| format | Artículo científico |
| id | pubmed_41661452 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Acta parasitologica |
| record_format | pubmed |
| spellingShingle | Prevalence and Subtypes of Blastocystis in Symptomatic Children in Egypt and Anti-Blastocystis Efficacy of Artemisia alba-Loaded Silver Nanoparticles In Vitro and in Swiss Albino Mice. Naguib, Doaa Alhussainy, Nabeel H El-Wahsh, Hany M Al Braikan, Faten A Basalem, Osama Salem Mal, Walaa Abdulsamad Ahmed, Ashraf Fawzy Mosa Baakdah, Fadi Alshumrani, Mohammad Mousa Darwish, Wael Sobhy Bakhsh, Wesam Kamal Prevalence and Subtypes of Blastocystis in Symptomatic Children in Egypt and Anti-Blastocystis Efficacy of Artemisia alba-Loaded Silver Nanoparticles In Vitro and in Swiss Albino Mice. Naguib, Doaa Alhussainy, Nabeel H El-Wahsh, Hany M Al Braikan, Faten A Basalem, Osama Salem Mal, Walaa Abdulsamad Ahmed, Ashraf Fawzy Mosa Baakdah, Fadi Alshumrani, Mohammad Mousa Darwish, Wael Sobhy Bakhsh, Wesam Kamal PURPOSE: Blastocystis hominis (B. hominis) is a common protozoan pathogen that affects the health of both humans and animals. This study aimed to investigate the prevalence of Blastocystis in children and evaluate the effects of Artemisia alba aqueous extract-loaded silver nanoparticles (AgNPs) on B. hominis infection, both in vitro and in mice. METHODS: Fresh fecal samples were collected from 235 children and analyzed for the presence of B. hominis using microscopy and culture techniques. Fifty positive samples were further subtyped using subtype-specific primers through PCR. Additionally, the effectiveness of Artemisia alba aqueous extract-loaded AgNPs in treating B. hominis infection was assessed both in vitro and in Swiss albino mice. RESULTS: Of the 235 children's fecal samples, 75 (32%) tested positive for B. hominis. Genotyping of 50 positive samples revealed that ST3 was the most common genotype, followed by ST1, ST2, and ST4. The results of the second phase showed that treatment with Artemisia alba aqueous extract-loaded AgNPs significantly inhibited the growth of Blastocystis in culture. Additionally, it significantly reduced the number of Blastocystis cysts found in the feces of treated mice. Furthermore, this treatment had minimal adverse effects on the liver, kidney, and intestine, especially when compared to metronidazole. CONCLUSION: Blastocystis is commonly found among children, with subtype ST3 being the most prevalent. The administration of Artemisia alba aqueous extract-loaded AgNPs demonstrated significant anti-Blastocystis activity, both in vitro and in vivo. Furthermore, the treatment was shown to be safe and may represent a promising option for addressing blastocystosis. |
| title | Prevalence and Subtypes of Blastocystis in Symptomatic Children in Egypt and Anti-Blastocystis Efficacy of Artemisia alba-Loaded Silver Nanoparticles In Vitro and in Swiss Albino Mice. |
| url | https://pubmed.ncbi.nlm.nih.gov/41661452/ |