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Main Authors: Ghayth Alzwawy, Ateeqah, Khazri, Abdelhafidh, Kouki, Ahmed, Ben Ali, Manel, Ben-Attia, Mossadok, Mahmoudi, Ezzeddine, Sakly, Mohsen, Sellami, Badreddine
Format: Artículo científico
Language:en
Published: Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals 2026
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Online Access:https://pubmed.ncbi.nlm.nih.gov/41664449/
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author Ghayth Alzwawy, Ateeqah
Khazri, Abdelhafidh
Kouki, Ahmed
Ben Ali, Manel
Ben-Attia, Mossadok
Mahmoudi, Ezzeddine
Sakly, Mohsen
Sellami, Badreddine
author_facet Ghayth Alzwawy, Ateeqah
Khazri, Abdelhafidh
Kouki, Ahmed
Ben Ali, Manel
Ben-Attia, Mossadok
Mahmoudi, Ezzeddine
Sakly, Mohsen
Sellami, Badreddine
Ghayth Alzwawy, Ateeqah
Khazri, Abdelhafidh
Kouki, Ahmed
Ben Ali, Manel
Ben-Attia, Mossadok
Mahmoudi, Ezzeddine
Sakly, Mohsen
Sellami, Badreddine
collection PubMed - marine biology
contents Evaluation of the protective effects of selenium against iron overload-induced toxicity in rats using a multiple-markers approach. Ghayth Alzwawy, Ateeqah Khazri, Abdelhafidh Kouki, Ahmed Ben Ali, Manel Ben-Attia, Mossadok Mahmoudi, Ezzeddine Sakly, Mohsen Sellami, Badreddine Animals Iron Overload Rats, Wistar Oxidative Stress Biomarkers Rats Liver Selenium Glutathione Male Glutathione Transferase Catalase Malondialdehyde Iron Kidney Antioxidants Spleen Acetylcholinesterase Pancreas Iron overload can promote the generation of reactive oxygen species (ROS), leading to oxidative stress and different human diseases. The trace element selenium has biological functions and can act as both an antioxidant and a prooxidant. This study aimed to evaluate the protective effects of selenium against iron overload-induced toxicity in rats. Adult Wistar rats were exposed to three increasing concentrations of iron (25, 50, and 100 mg/kg body weight [b.w.]), either alone or in combination with selenium (0.5 mg/kg b.w.). The biological interactions between these two compounds were investigated at the biochemical level in the liver, spleen, kidney, and pancreas. Our results indicated that iron used alone induces oxidative stress. In all tissues studied and at all the administered doses, we observed changes in the levels of catalase (CAT), glutathione (GSH), glutathione-S-transferase (GST), malondialdehyde (MDA), and acetylcholinesterase (AChE). The responses were dose- and organ-dependent. Selenium administered at 0.5 mg/kg b.w. attenuate the adverse effects of the different iron dosages. These findings highlight the potential application of selenium in mitigating oxidative stress and organ toxicity associated with iron overload. Our research carries significant implications for the development of nutritional and therapeutic strategies aimed at managing disorders related to iron metabolism.
format Artículo científico
id pubmed_41664449
institution PubMed
language en
publishDate 2026
publisher Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals
record_format pubmed
spellingShingle Evaluation of the protective effects of selenium against iron overload-induced toxicity in rats using a multiple-markers approach.
Ghayth Alzwawy, Ateeqah
Khazri, Abdelhafidh
Kouki, Ahmed
Ben Ali, Manel
Ben-Attia, Mossadok
Mahmoudi, Ezzeddine
Sakly, Mohsen
Sellami, Badreddine
Animals
Iron Overload
Rats, Wistar
Oxidative Stress
Biomarkers
Rats
Liver
Selenium
Glutathione
Male
Glutathione Transferase
Catalase
Malondialdehyde
Iron
Kidney
Antioxidants
Spleen
Acetylcholinesterase
Pancreas
Evaluation of the protective effects of selenium against iron overload-induced toxicity in rats using a multiple-markers approach. Ghayth Alzwawy, Ateeqah Khazri, Abdelhafidh Kouki, Ahmed Ben Ali, Manel Ben-Attia, Mossadok Mahmoudi, Ezzeddine Sakly, Mohsen Sellami, Badreddine Animals Iron Overload Rats, Wistar Oxidative Stress Biomarkers Rats Liver Selenium Glutathione Male Glutathione Transferase Catalase Malondialdehyde Iron Kidney Antioxidants Spleen Acetylcholinesterase Pancreas Iron overload can promote the generation of reactive oxygen species (ROS), leading to oxidative stress and different human diseases. The trace element selenium has biological functions and can act as both an antioxidant and a prooxidant. This study aimed to evaluate the protective effects of selenium against iron overload-induced toxicity in rats. Adult Wistar rats were exposed to three increasing concentrations of iron (25, 50, and 100 mg/kg body weight [b.w.]), either alone or in combination with selenium (0.5 mg/kg b.w.). The biological interactions between these two compounds were investigated at the biochemical level in the liver, spleen, kidney, and pancreas. Our results indicated that iron used alone induces oxidative stress. In all tissues studied and at all the administered doses, we observed changes in the levels of catalase (CAT), glutathione (GSH), glutathione-S-transferase (GST), malondialdehyde (MDA), and acetylcholinesterase (AChE). The responses were dose- and organ-dependent. Selenium administered at 0.5 mg/kg b.w. attenuate the adverse effects of the different iron dosages. These findings highlight the potential application of selenium in mitigating oxidative stress and organ toxicity associated with iron overload. Our research carries significant implications for the development of nutritional and therapeutic strategies aimed at managing disorders related to iron metabolism.
title Evaluation of the protective effects of selenium against iron overload-induced toxicity in rats using a multiple-markers approach.
topic Animals
Iron Overload
Rats, Wistar
Oxidative Stress
Biomarkers
Rats
Liver
Selenium
Glutathione
Male
Glutathione Transferase
Catalase
Malondialdehyde
Iron
Kidney
Antioxidants
Spleen
Acetylcholinesterase
Pancreas
url https://pubmed.ncbi.nlm.nih.gov/41664449/