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| Format: | Artículo científico |
| Sprache: | en |
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Journal of natural products
2026
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| Schlagworte: | |
| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/41677145/ |
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| author | McAtamney, Allyson Lagousis, Cayla Colquitt, Bradley M Pita, Lucia Eustaquio, Alessandra S Sanchez, Laura M |
| author_facet | McAtamney, Allyson Lagousis, Cayla Colquitt, Bradley M Pita, Lucia Eustaquio, Alessandra S Sanchez, Laura M McAtamney, Allyson Lagousis, Cayla Colquitt, Bradley M Pita, Lucia Eustaquio, Alessandra S Sanchez, Laura M |
| collection | PubMed - marine biology |
| contents | MALDI-FISH for Colocalization of Brominated Metabolites and spp. in Tissue. McAtamney, Allyson Lagousis, Cayla Colquitt, Bradley M Pita, Lucia Eustaquio, Alessandra S Sanchez, Laura M Animals Porifera In Situ Hybridization, Fluorescence Tyrosine Molecular Structure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Rhodobacteraceae Bromotyrosine-containing natural products have been isolated from marine sponges. While studying the uptake of engineered bacterial strains by sponges, we observed the production of fistularin-3 in the sponge. To explore these results, we employed multimodal imaging to colocalize metabolites with fluorescent in situ hybridization (FISH) probes. Using mass spectrometry imaging (MSI), we measured fistularin-3 and mapped this against the FISH probes to locate the bacteria. A recent study reported that produced fistularin-3 in pure culture, but these data have not yet been reproduced. We employed MSI and FISH to colocalize the fistularin-3 spatial distribution with in sponge tissue. |
| format | Artículo científico |
| id | pubmed_41677145 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Journal of natural products |
| record_format | pubmed |
| spellingShingle | MALDI-FISH for Colocalization of Brominated Metabolites and spp. in Tissue. McAtamney, Allyson Lagousis, Cayla Colquitt, Bradley M Pita, Lucia Eustaquio, Alessandra S Sanchez, Laura M Animals Porifera In Situ Hybridization, Fluorescence Tyrosine Molecular Structure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Rhodobacteraceae MALDI-FISH for Colocalization of Brominated Metabolites and spp. in Tissue. McAtamney, Allyson Lagousis, Cayla Colquitt, Bradley M Pita, Lucia Eustaquio, Alessandra S Sanchez, Laura M Animals Porifera In Situ Hybridization, Fluorescence Tyrosine Molecular Structure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Rhodobacteraceae Bromotyrosine-containing natural products have been isolated from marine sponges. While studying the uptake of engineered bacterial strains by sponges, we observed the production of fistularin-3 in the sponge. To explore these results, we employed multimodal imaging to colocalize metabolites with fluorescent in situ hybridization (FISH) probes. Using mass spectrometry imaging (MSI), we measured fistularin-3 and mapped this against the FISH probes to locate the bacteria. A recent study reported that produced fistularin-3 in pure culture, but these data have not yet been reproduced. We employed MSI and FISH to colocalize the fistularin-3 spatial distribution with in sponge tissue. |
| title | MALDI-FISH for Colocalization of Brominated Metabolites and spp. in Tissue. |
| topic | Animals Porifera In Situ Hybridization, Fluorescence Tyrosine Molecular Structure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Rhodobacteraceae |
| url | https://pubmed.ncbi.nlm.nih.gov/41677145/ |