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| Autori principali: | , , , , , |
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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Journal of natural products
2026
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| Soggetti: | |
| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/41677145/ |
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Sommario:
- MALDI-FISH for Colocalization of Brominated Metabolites and spp. in Tissue. McAtamney, Allyson Lagousis, Cayla Colquitt, Bradley M Pita, Lucia Eustaquio, Alessandra S Sanchez, Laura M Animals Porifera In Situ Hybridization, Fluorescence Tyrosine Molecular Structure Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Rhodobacteraceae Bromotyrosine-containing natural products have been isolated from marine sponges. While studying the uptake of engineered bacterial strains by sponges, we observed the production of fistularin-3 in the sponge. To explore these results, we employed multimodal imaging to colocalize metabolites with fluorescent in situ hybridization (FISH) probes. Using mass spectrometry imaging (MSI), we measured fistularin-3 and mapped this against the FISH probes to locate the bacteria. A recent study reported that produced fistularin-3 in pure culture, but these data have not yet been reproduced. We employed MSI and FISH to colocalize the fistularin-3 spatial distribution with in sponge tissue.