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| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Biology
2026
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41677680/ |
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Table of Contents:
- Identification of Genes Associated with the Pan-Vibrios Resistance (PVR) Trait of Pacific White Shrimp () Using a Genome-Wide Association Study. Wen, Shuyang Cheng, Chuhang Yin, Jiayue Lv, Ying Zhang, Xin Ma, Bo Liu, Yang Qiu, Yueshan He, Huteng Luo, Peng Yuan, Lihong Vibriosis, caused by diverse species, is among the most devastating bacterial diseases in shrimp aquaculture. Consequently, breeding shrimp for pan-vibrios resistance (PVR) presents a crucial strategy for sustainable shrimp farming. In this work, we performed a GWAS in to identify genetic loci underlying resistance to pan-vibrios and validate the identified SNPs. A total of 300 shrimp from nine different regions were subjected to a comprehensive challenge. Selective genotyping of 300 resistant and susceptible individuals was conducted using a specific length amplified fragment sequencing (SLAF-seq) approach. A total of 18,184,608 high-quality SNPs were detected across the whole genome of . Screening identified 283 SNPs located within genes, 26 of which were associated with the PVR trait. These SNPs were subsequently validated in verification group of 80 shrimps, leading to the identification of two genotypes (GG at SNP20 and AA at SNP21) and one genotype combination (GG/AA at SNP20 and SNP21) that were significantly associated with the PVR trait. Notably, these linked SNPs were identified in the intron of gene. The highest expression was observed in immune-related tissues including hemocytes, the gills, and the hepatopancreas. Furthermore, qPCR results showed that expression was significantly higher in the vibrios-resistant (RES) group than in the vibrios-susceptible (SUS) group. This study proposed the PVR concept and provided valuable molecular markers for the genetic improvement of vibrios-resistance in .