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| Main Authors: | , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Biology
2026
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41677690/ |
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Table of Contents:
- Multiplex qPCR Assay for Simultaneous Detection of Three PST-Producing Dinoflagellates in the East China Sea off Southern Korea. Heo, Jung Soo Tran, Biet Thanh Kim, Keun-Yong Kim, Sunju Youn, Seok Hyun Park, Tae Gyu Paralytic shellfish toxins (PSTs) are produced by several toxic species of the dinoflagellate genera and , and they pose significant threats to marine ecosystems and public health. Rapid and accurate detection of harmful algal blooms (HABs) is essential for effective management. In this study, we developed a multiplex quantitative real-time PCR (qPCR) assay targeting the 28S ribosomal DNA region to simultaneously detect three PST-producing dinoflagellates, , , and , in the East China Sea off southern Korea. Species-specific primers and hydrolysis probes labeled with distinct fluorophores were validated for simultaneous detection. The standard curves showed strong linearity ( > 0.99) and high amplification efficiencies (95.268-99.325%). No cross-reactivity was observed among the 20 non-target microalgal species. Field application of the assay using environmental DNA (eDNA) samples collected during spring successfully detected and , whereas was not detected during the survey period. This multiplex qPCR assay provides a rapid and reliable molecular tool for early detection and spatial monitoring of potentially PST-producing dinoflagellates, supporting sustainable HAB management in East Asian coastal ecosystems.