PRICE: direct and robust detection of microRNAs at single-nucleotide resolution.
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| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
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Nature communications
2026
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| author | Wang, Buhua Zhou, Shuai Zhang, Xi Wang, Rui Huang, Shuo Li, Anyi He, Haowei Zhang, Yingai Wei, Yangdao Yang, Zhiqing Song, Fengge Li, Xiangpeng Wan, Xinyi Shen, Yi Ma, Chunxin Mao, Haimei Ledesma-Amaro, Rodrigo Yin, Dapeng Wei, Qingshan Deng, Ruijie Yang, Tao Liu, Shanrong Wan, Yi Mao, Chuanbin |
| author_facet | Wang, Buhua Zhou, Shuai Zhang, Xi Wang, Rui Huang, Shuo Li, Anyi He, Haowei Zhang, Yingai Wei, Yangdao Yang, Zhiqing Song, Fengge Li, Xiangpeng Wan, Xinyi Shen, Yi Ma, Chunxin Mao, Haimei Ledesma-Amaro, Rodrigo Yin, Dapeng Wei, Qingshan Deng, Ruijie Yang, Tao Liu, Shanrong Wan, Yi Mao, Chuanbin Wang, Buhua Zhou, Shuai Zhang, Xi Wang, Rui Huang, Shuo Li, Anyi He, Haowei Zhang, Yingai Wei, Yangdao Yang, Zhiqing Song, Fengge Li, Xiangpeng Wan, Xinyi Shen, Yi Ma, Chunxin Mao, Haimei Ledesma-Amaro, Rodrigo Yin, Dapeng Wei, Qingshan Deng, Ruijie Yang, Tao Liu, Shanrong Wan, Yi Mao, Chuanbin |
| collection | PubMed - marine biology |
| contents | PRICE: direct and robust detection of microRNAs at single-nucleotide resolution. Wang, Buhua Zhou, Shuai Zhang, Xi Wang, Rui Huang, Shuo Li, Anyi He, Haowei Zhang, Yingai Wei, Yangdao Yang, Zhiqing Song, Fengge Li, Xiangpeng Wan, Xinyi Shen, Yi Ma, Chunxin Mao, Haimei Ledesma-Amaro, Rodrigo Yin, Dapeng Wei, Qingshan Deng, Ruijie Yang, Tao Liu, Shanrong Wan, Yi Mao, Chuanbin MicroRNAs Peptide Nucleic Acids Humans CRISPR-Cas Systems Polymorphism, Single Nucleotide Accurate single-nucleotide discrimination of miRNA is clinically vital because small sequence variations can have significant phenotypic and clinical consequences, yet existing techniques can only detect single nucleotide variations (SNVs) at specific loci. Here, we present a generalized peptide nucleic acid (PNA) mediated CRISPR/Cas13a system (PRICE), enabling detection of SNVs in miRNA sequence without sacrificing the sensitivity. PRICE utilizes PNA blockers fully complementary to non-target miRNAs (e.g., miRNAs containing SNVs at loci of no interest) but not to the target miRNA. These blockers selectively hybridize with and inhibit non-target sequences in samples (serum, cells, or tissues). Only the unhybridized target miRNA then binds to crRNA within the Cas13a complex, activating Cas13a to cleave a fluorescent reporter-quencher linker, generating a detectable signal (~10 fM limit). By designing a panel of PNAs against SNVs, PRICE provides a versatile, amplification-free platform for precise miRNA analysis, advancing cancer diagnosis, prognosis, and biology. |
| format | Artículo científico |
| id | pubmed_41680160 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Nature communications |
| record_format | pubmed |
| spellingShingle | PRICE: direct and robust detection of microRNAs at single-nucleotide resolution. Wang, Buhua Zhou, Shuai Zhang, Xi Wang, Rui Huang, Shuo Li, Anyi He, Haowei Zhang, Yingai Wei, Yangdao Yang, Zhiqing Song, Fengge Li, Xiangpeng Wan, Xinyi Shen, Yi Ma, Chunxin Mao, Haimei Ledesma-Amaro, Rodrigo Yin, Dapeng Wei, Qingshan Deng, Ruijie Yang, Tao Liu, Shanrong Wan, Yi Mao, Chuanbin MicroRNAs Peptide Nucleic Acids Humans CRISPR-Cas Systems Polymorphism, Single Nucleotide PRICE: direct and robust detection of microRNAs at single-nucleotide resolution. Wang, Buhua Zhou, Shuai Zhang, Xi Wang, Rui Huang, Shuo Li, Anyi He, Haowei Zhang, Yingai Wei, Yangdao Yang, Zhiqing Song, Fengge Li, Xiangpeng Wan, Xinyi Shen, Yi Ma, Chunxin Mao, Haimei Ledesma-Amaro, Rodrigo Yin, Dapeng Wei, Qingshan Deng, Ruijie Yang, Tao Liu, Shanrong Wan, Yi Mao, Chuanbin MicroRNAs Peptide Nucleic Acids Humans CRISPR-Cas Systems Polymorphism, Single Nucleotide Accurate single-nucleotide discrimination of miRNA is clinically vital because small sequence variations can have significant phenotypic and clinical consequences, yet existing techniques can only detect single nucleotide variations (SNVs) at specific loci. Here, we present a generalized peptide nucleic acid (PNA) mediated CRISPR/Cas13a system (PRICE), enabling detection of SNVs in miRNA sequence without sacrificing the sensitivity. PRICE utilizes PNA blockers fully complementary to non-target miRNAs (e.g., miRNAs containing SNVs at loci of no interest) but not to the target miRNA. These blockers selectively hybridize with and inhibit non-target sequences in samples (serum, cells, or tissues). Only the unhybridized target miRNA then binds to crRNA within the Cas13a complex, activating Cas13a to cleave a fluorescent reporter-quencher linker, generating a detectable signal (~10 fM limit). By designing a panel of PNAs against SNVs, PRICE provides a versatile, amplification-free platform for precise miRNA analysis, advancing cancer diagnosis, prognosis, and biology. |
| title | PRICE: direct and robust detection of microRNAs at single-nucleotide resolution. |
| topic | MicroRNAs Peptide Nucleic Acids Humans CRISPR-Cas Systems Polymorphism, Single Nucleotide |
| url | https://pubmed.ncbi.nlm.nih.gov/41680160/ |