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| Autori principali: | , , , , , , |
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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
International journal of molecular sciences
2026
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| Soggetti: | |
| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/41684017/ |
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| _version_ | 1868266084895817730 |
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| author | Ben Ammar, Hajer Kabtni, Souhir Arena, Donata Amari, Marwen Al Achkar, Nicolas Branca, Ferdinando Marghali, Sonia |
| author_facet | Ben Ammar, Hajer Kabtni, Souhir Arena, Donata Amari, Marwen Al Achkar, Nicolas Branca, Ferdinando Marghali, Sonia Ben Ammar, Hajer Kabtni, Souhir Arena, Donata Amari, Marwen Al Achkar, Nicolas Branca, Ferdinando Marghali, Sonia |
| collection | PubMed - marine biology |
| contents | Integrating Metabolic and Gene Expression Profiling of Glucosinolate Biosynthesis Under Drought Stress in . Ben Ammar, Hajer Kabtni, Souhir Arena, Donata Amari, Marwen Al Achkar, Nicolas Branca, Ferdinando Marghali, Sonia Brassica Glucosinolates Gene Expression Regulation, Plant Gene Expression Profiling Droughts Stress, Physiological Plant Proteins Drought Resistance Promoter Regions, Genetic Drought stress induces pronounced metabolic and transcriptional reprogramming of glucosinolate (GLS) biosynthesis in . An integrative approach combining HPLC-based quantification of individual GLSs, quantitative real-time PCR of core biosynthetic and regulatory genes, correlation-based network analysis, and in silico promoter characterization was applied to evaluate drought responses across genetically diverse accessions. Drought triggered strong, accession-specific shifts in GLS composition, with sinigrin content increasing from 35.9% to 55.1% in BR1 and glucoerucin reaching up to 80.2% in CCP1, while indolic GLSs such as glucobrassicin and neoglucobrassicin accounted for >75% of total GLSs in CV2 and CCP3. Hierarchical clustering separated accessions into four distinct drought response clusters independent of morphotype. Correlation analysis revealed drought-induced rewiring of GLS interdependencies, characterized by strengthened positive associations among aliphatic GLSs (r > 0.75). Gene expression profiling identified conserved MYB-centered regulatory modules (, , , ) alongside strong accession-specific induction of (up to 6.3-fold), (up to 4.8-fold), and (up to 5.1-fold). Promoter analysis revealed enrichment of ABA- and stress-responsive cis-regulatory elements. These findings delineate a genotype-dependent regulatory framework underlying GLS plasticity and identify quantitative metabolic and transcriptional markers relevant for breeding drought-resilient cultivars. |
| format | Artículo científico |
| id | pubmed_41684017 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | International journal of molecular sciences |
| record_format | pubmed |
| spellingShingle | Integrating Metabolic and Gene Expression Profiling of Glucosinolate Biosynthesis Under Drought Stress in . Ben Ammar, Hajer Kabtni, Souhir Arena, Donata Amari, Marwen Al Achkar, Nicolas Branca, Ferdinando Marghali, Sonia Brassica Glucosinolates Gene Expression Regulation, Plant Gene Expression Profiling Droughts Stress, Physiological Plant Proteins Drought Resistance Promoter Regions, Genetic Integrating Metabolic and Gene Expression Profiling of Glucosinolate Biosynthesis Under Drought Stress in . Ben Ammar, Hajer Kabtni, Souhir Arena, Donata Amari, Marwen Al Achkar, Nicolas Branca, Ferdinando Marghali, Sonia Brassica Glucosinolates Gene Expression Regulation, Plant Gene Expression Profiling Droughts Stress, Physiological Plant Proteins Drought Resistance Promoter Regions, Genetic Drought stress induces pronounced metabolic and transcriptional reprogramming of glucosinolate (GLS) biosynthesis in . An integrative approach combining HPLC-based quantification of individual GLSs, quantitative real-time PCR of core biosynthetic and regulatory genes, correlation-based network analysis, and in silico promoter characterization was applied to evaluate drought responses across genetically diverse accessions. Drought triggered strong, accession-specific shifts in GLS composition, with sinigrin content increasing from 35.9% to 55.1% in BR1 and glucoerucin reaching up to 80.2% in CCP1, while indolic GLSs such as glucobrassicin and neoglucobrassicin accounted for >75% of total GLSs in CV2 and CCP3. Hierarchical clustering separated accessions into four distinct drought response clusters independent of morphotype. Correlation analysis revealed drought-induced rewiring of GLS interdependencies, characterized by strengthened positive associations among aliphatic GLSs (r > 0.75). Gene expression profiling identified conserved MYB-centered regulatory modules (, , , ) alongside strong accession-specific induction of (up to 6.3-fold), (up to 4.8-fold), and (up to 5.1-fold). Promoter analysis revealed enrichment of ABA- and stress-responsive cis-regulatory elements. These findings delineate a genotype-dependent regulatory framework underlying GLS plasticity and identify quantitative metabolic and transcriptional markers relevant for breeding drought-resilient cultivars. |
| title | Integrating Metabolic and Gene Expression Profiling of Glucosinolate Biosynthesis Under Drought Stress in . |
| topic | Brassica Glucosinolates Gene Expression Regulation, Plant Gene Expression Profiling Droughts Stress, Physiological Plant Proteins Drought Resistance Promoter Regions, Genetic |
| url | https://pubmed.ncbi.nlm.nih.gov/41684017/ |