Emergence of Hypervirulent, Multidrug-Resistant Klebsiella pneumoniae Harboring magA in Coastal Water Sources: A Public Health Threat in Flood-Prone Communities.
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| Auteurs principaux: | , , , |
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| Format: | Artículo científico |
| Langue: | en |
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Water environment research : a research publication of the Water Environment Federation
2026
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| _version_ | 1868266084876943361 |
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| author | Yousuf, Jesmi Aneesa, P A Mujeeb Rahiman, K M Mohamed Hatha, A A |
| author_facet | Yousuf, Jesmi Aneesa, P A Mujeeb Rahiman, K M Mohamed Hatha, A A Yousuf, Jesmi Aneesa, P A Mujeeb Rahiman, K M Mohamed Hatha, A A |
| collection | PubMed - marine biology |
| contents | Emergence of Hypervirulent, Multidrug-Resistant Klebsiella pneumoniae Harboring magA in Coastal Water Sources: A Public Health Threat in Flood-Prone Communities. Yousuf, Jesmi Aneesa, P A Mujeeb Rahiman, K M Mohamed Hatha, A A Klebsiella pneumoniae Drug Resistance, Multiple, Bacterial Anti-Bacterial Agents Floods Microbial Sensitivity Tests India Biofilms Virulence RNA, Ribosomal, 16S Water Microbiology Coastal flooding increasingly compromises water security while accelerating antimicrobial resistance through enhanced microbial gene transfer. In light of growing concerns over the widespread occurrence of multidrug-resistant Klebsiella pneumoniae in natural environments, we investigated the emergence and specific traits of classical and hypervirulent phenotypes of K. pneumoniae from domestic water sources in Cochin, India, a region frequently affected by flooding. Isolates were identified using 16S rRNA gene sequencing. Phenotypic characterization included the string test for hypermucoviscous traits, while antimicrobial susceptibility was determined via disc diffusion and microdilution. The ESBL production was confirmed using the combined disc diffusion test. Biofilm formation was quantified via the microtiter plate method with crystal violet staining. Finally, PCR was employed to detect key resistance (bla, bla, and blao) and virulence (magA) genes. Findings revealed that 95% of the isolates were multidrug resistant, with 75% confirmed as ESBLs and 42% displaying hypermucoviscous phenotype. Resistance was observed against critically important antibiotics, including carbapenems and colistin, with the highest Minimum inhibitory concentration (MIC) values against ceftazidime and ampicillin (> 256 μg/mL). Hypervirulent strains exhibited significantly higher resistance levels and formed thicker biofilms compared to classical strains (p = 0.01) and highly correlated with multiple antibiotic resistance index (ρ = 0.90, p |
| format | Artículo científico |
| id | pubmed_41699885 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Water environment research : a research publication of the Water Environment Federation |
| record_format | pubmed |
| spellingShingle | Emergence of Hypervirulent, Multidrug-Resistant Klebsiella pneumoniae Harboring magA in Coastal Water Sources: A Public Health Threat in Flood-Prone Communities. Yousuf, Jesmi Aneesa, P A Mujeeb Rahiman, K M Mohamed Hatha, A A Klebsiella pneumoniae Drug Resistance, Multiple, Bacterial Anti-Bacterial Agents Floods Microbial Sensitivity Tests India Biofilms Virulence RNA, Ribosomal, 16S Water Microbiology Emergence of Hypervirulent, Multidrug-Resistant Klebsiella pneumoniae Harboring magA in Coastal Water Sources: A Public Health Threat in Flood-Prone Communities. Yousuf, Jesmi Aneesa, P A Mujeeb Rahiman, K M Mohamed Hatha, A A Klebsiella pneumoniae Drug Resistance, Multiple, Bacterial Anti-Bacterial Agents Floods Microbial Sensitivity Tests India Biofilms Virulence RNA, Ribosomal, 16S Water Microbiology Coastal flooding increasingly compromises water security while accelerating antimicrobial resistance through enhanced microbial gene transfer. In light of growing concerns over the widespread occurrence of multidrug-resistant Klebsiella pneumoniae in natural environments, we investigated the emergence and specific traits of classical and hypervirulent phenotypes of K. pneumoniae from domestic water sources in Cochin, India, a region frequently affected by flooding. Isolates were identified using 16S rRNA gene sequencing. Phenotypic characterization included the string test for hypermucoviscous traits, while antimicrobial susceptibility was determined via disc diffusion and microdilution. The ESBL production was confirmed using the combined disc diffusion test. Biofilm formation was quantified via the microtiter plate method with crystal violet staining. Finally, PCR was employed to detect key resistance (bla, bla, and blao) and virulence (magA) genes. Findings revealed that 95% of the isolates were multidrug resistant, with 75% confirmed as ESBLs and 42% displaying hypermucoviscous phenotype. Resistance was observed against critically important antibiotics, including carbapenems and colistin, with the highest Minimum inhibitory concentration (MIC) values against ceftazidime and ampicillin (> 256 μg/mL). Hypervirulent strains exhibited significantly higher resistance levels and formed thicker biofilms compared to classical strains (p = 0.01) and highly correlated with multiple antibiotic resistance index (ρ = 0.90, p |
| title | Emergence of Hypervirulent, Multidrug-Resistant Klebsiella pneumoniae Harboring magA in Coastal Water Sources: A Public Health Threat in Flood-Prone Communities. |
| topic | Klebsiella pneumoniae Drug Resistance, Multiple, Bacterial Anti-Bacterial Agents Floods Microbial Sensitivity Tests India Biofilms Virulence RNA, Ribosomal, 16S Water Microbiology |
| url | https://pubmed.ncbi.nlm.nih.gov/41699885/ |