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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
The Science of the total environment
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41701995/ |
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Table of Contents:
- Acidic-alkaline shocks in vinasse fermentation shape methanogenesis and sulfate reduction dynamics. do Vale Borges, André Fuess, Lucas Tadeu Dornelles, Henrique Takeda, Paula Yumi Saia, Flávia Talarico Rogeri, Renan Coghi Gomes, Kaio Gustavo Damianovic, Márcia Helena Rissato Zamariolli Methane Sulfates Fermentation Bioreactors Hydrogen-Ion Concentration Saccharum Anaerobiosis Waste Disposal, Fluid Oxidation-Reduction Efficient two-phase anaerobic digestion (2nd-AD) of sugarcane vinasse hinges on effectively suppressing methanogenesis within the initial sulfate-reducing stage (acidogenesis) to maximize downstream methane production and mitigate safety risks associated with HS/CH co-production. This study investigates the strategic in-process application of sequential acidic and alkaline pH shocks to achieve this critical control. An anaerobic structured-bed reactor (AnSTBR), reactivated from prolonged storage (5 months) to mimic off-season conditions and test long-term system resilience, was fed with vinasse for 90 days at 30 °C across six operational stages. The results demonstrate that these pH shocks hindered the hydrogenotrophic and, mainly, acetoclastic methanogenesis, reducing methane content to 3% while restoring sulfidogenesis to 82% (Stage VI) even after the system returned to original conditions. This robust sulfate removal in high-rate fermentative systems yielded an effluent rich in acetate (>3.0 g-HAc L) with enhanced buffering capacity, ideal for subsequent acetoclastic methanogenesis. Microbial community analysis identified Desulfovibrio (28.69-49.38%) as the dominant and most active dissimilatory sulfate reducer, while Bacteroides (6.49-3.44%) and Aminobacterium (1.73-8.82%) were key acetate producers driving fermentative metabolism. This work establishes a novel operational strategy to efficiently modulate microbial pathways in vinasse biorefineries, advancing biogas production, environmental protection, and sustainable waste management.