Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Dental materials : official publication of the Academy of Dental Materials
2026
|
| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41702774/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Table of Contents:
- Structural analysis of a collagen-fibrin scaffold which promotes osteogenic differentiation and templated biomineralization of dental pulp stem cells. Luo, Huiting Fu, Shi Cui, Muyun Feng, Kuan-Che Wong, Robert Hansen, Adam Zaliznyak, Tatiana Sprouster, David Kleinerman, Olga Singh, Gurtej Cymerman, Jerome Simon, Marcia Rafailovich, Miriam Dental Pulp Cell Differentiation Stem Cells Tissue Scaffolds Osteogenesis Collagen Humans Spectrum Analysis, Raman Fibrin Microscopy, Electron, Scanning Cells, Cultured Biomineralization Microscopy, Atomic Force Hydrogels Clinical regenerative endodontic treatment (RET) studies report enhanced intracanal mineralization when collagen-based scaffolds are combined with blood, but the underlying mechanisms remain unclear. This study used a simplified in vitro collagen-fibrin model, incorporating fibrin, a key blood component, into collagen. The structural, chemical, and mechanical properties of collagen-fibrin scaffolds were compared with those of the individual components and correlated with osteogenic differentiation and biomineralization by dental pulp stem cells (DPSCs). Collagen, fibrin, and collagen-fibrin hydrogels were fabricated and characterized by Raman spectroscopy, cryo-scanning electron microscopy (cryo-SEM), atomic force microscopy (AFM), and oscillatory shear rheology. DPSCs were cultured on the scaffolds for up to 28 days without dexamethasone to isolate scaffold-driven effects. Osteogenic differentiation and mineralization were evaluated by qRT-PCR, Raman spectroscopy, Alizarin Red S (ARS) staining, and SEM-EDX. Collagen-fibrin scaffolds formed composite networks with increased modulus and distinct microstructure relative to the individual hydrogels. DPSCs cultured on collagen-fibrin scaffolds exhibited enhanced osteogenic differentiation, including increased expression of osteocalcin and bone sialoprotein. Although calcium phosphate formed on all scaffolds, collagen-fibrin composites uniquely supported templated, fiber-associated hydroxyapatite with crystallinity comparable to native bone. Collagen-fibrin hydrogels provide a mechanically reinforced, extracellular matrix-mimicking scaffold that promotes biologically regulated mineralization by DPSCs. This model offers mechanistic insight into clinically observed hard tissue formation in RET and highlights fibrin as a key contributor to mineralized tissue formation, with translational relevance when bleeding induction is limited.