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| Sprache: | en |
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Nature communications
2026
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/41708610/ |
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| author | Klann, Marleen Miura, Saori Lee, Shu-Hua Vianello, Stefano Davide Ross, Robert Watanabe, Masakatsu Gairin, Emma Liang, Yipeng Hutto, Harrison W McCluskey, Braedan M Herrera, Marcela Solnica-Krezel, Lila Besseau, Laurence Pigolotti, Simone Parichy, David M Kinoshita, Masato Laudet, Vincent |
| author_facet | Klann, Marleen Miura, Saori Lee, Shu-Hua Vianello, Stefano Davide Ross, Robert Watanabe, Masakatsu Gairin, Emma Liang, Yipeng Hutto, Harrison W McCluskey, Braedan M Herrera, Marcela Solnica-Krezel, Lila Besseau, Laurence Pigolotti, Simone Parichy, David M Kinoshita, Masato Laudet, Vincent Klann, Marleen Miura, Saori Lee, Shu-Hua Vianello, Stefano Davide Ross, Robert Watanabe, Masakatsu Gairin, Emma Liang, Yipeng Hutto, Harrison W McCluskey, Braedan M Herrera, Marcela Solnica-Krezel, Lila Besseau, Laurence Pigolotti, Simone Parichy, David M Kinoshita, Masato Laudet, Vincent |
| collection | PubMed - marine biology |
| contents | Cell-cell communication as underlying principle governing color pattern formation in teleost fishes. Klann, Marleen Miura, Saori Lee, Shu-Hua Vianello, Stefano Davide Ross, Robert Watanabe, Masakatsu Gairin, Emma Liang, Yipeng Hutto, Harrison W McCluskey, Braedan M Herrera, Marcela Solnica-Krezel, Lila Besseau, Laurence Pigolotti, Simone Parichy, David M Kinoshita, Masato Laudet, Vincent Animals Connexins Cell Communication Zebrafish Pigmentation Gap Junctions Gap Junction alpha-5 Protein Fishes Mutation, Missense Fish Proteins Genome-Wide Association Study The diverse pigmentation patterns of animals are crucial for predation avoidance and behavioral display. This diversity arises from interactions among distinct pigment cell types, yet mechanisms generating pattern variation across teleost fishes remain incompletely understood. In zebrafish, Turing models have been proposed to explain stripe patterns, but it is unclear if they apply to other fishes. Here, we investigate the Snowflake mutant of the anemonefish Amphiprion ocellaris, which displays enlarged white bars with irregular boundaries. Using genome-wide association mapping and targeted sequencing, we identify a missense mutation (E42K) in gja5b, encoding the gap junction protein Connexin 41.8. CRISPR/Cas9-mediated genome editing recapitulates the Snowflake phenotype, while pharmacological inhibition of gap junctions phenocopies the boundary defects, supporting a causal role for impaired intercellular communication. Expression analyses reveal that, unlike zebrafish, anemonefish gja5b is predominantly expressed in iridophores. With functional in vitro assays we demonstrate that the E42K mutation acts as a dominant negative, strongly reducing gap junctional coupling. Introducing the same mutation in zebrafish reveals context-dependent effects on pigment patterning. Taken together our findings highlighting gap junction-mediated communication as a conserved but flexible mechanism controlling pigment boundary positioning and pattern diversification. |
| format | Artículo científico |
| id | pubmed_41708610 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Nature communications |
| record_format | pubmed |
| spellingShingle | Cell-cell communication as underlying principle governing color pattern formation in teleost fishes. Klann, Marleen Miura, Saori Lee, Shu-Hua Vianello, Stefano Davide Ross, Robert Watanabe, Masakatsu Gairin, Emma Liang, Yipeng Hutto, Harrison W McCluskey, Braedan M Herrera, Marcela Solnica-Krezel, Lila Besseau, Laurence Pigolotti, Simone Parichy, David M Kinoshita, Masato Laudet, Vincent Animals Connexins Cell Communication Zebrafish Pigmentation Gap Junctions Gap Junction alpha-5 Protein Fishes Mutation, Missense Fish Proteins Genome-Wide Association Study Cell-cell communication as underlying principle governing color pattern formation in teleost fishes. Klann, Marleen Miura, Saori Lee, Shu-Hua Vianello, Stefano Davide Ross, Robert Watanabe, Masakatsu Gairin, Emma Liang, Yipeng Hutto, Harrison W McCluskey, Braedan M Herrera, Marcela Solnica-Krezel, Lila Besseau, Laurence Pigolotti, Simone Parichy, David M Kinoshita, Masato Laudet, Vincent Animals Connexins Cell Communication Zebrafish Pigmentation Gap Junctions Gap Junction alpha-5 Protein Fishes Mutation, Missense Fish Proteins Genome-Wide Association Study The diverse pigmentation patterns of animals are crucial for predation avoidance and behavioral display. This diversity arises from interactions among distinct pigment cell types, yet mechanisms generating pattern variation across teleost fishes remain incompletely understood. In zebrafish, Turing models have been proposed to explain stripe patterns, but it is unclear if they apply to other fishes. Here, we investigate the Snowflake mutant of the anemonefish Amphiprion ocellaris, which displays enlarged white bars with irregular boundaries. Using genome-wide association mapping and targeted sequencing, we identify a missense mutation (E42K) in gja5b, encoding the gap junction protein Connexin 41.8. CRISPR/Cas9-mediated genome editing recapitulates the Snowflake phenotype, while pharmacological inhibition of gap junctions phenocopies the boundary defects, supporting a causal role for impaired intercellular communication. Expression analyses reveal that, unlike zebrafish, anemonefish gja5b is predominantly expressed in iridophores. With functional in vitro assays we demonstrate that the E42K mutation acts as a dominant negative, strongly reducing gap junctional coupling. Introducing the same mutation in zebrafish reveals context-dependent effects on pigment patterning. Taken together our findings highlighting gap junction-mediated communication as a conserved but flexible mechanism controlling pigment boundary positioning and pattern diversification. |
| title | Cell-cell communication as underlying principle governing color pattern formation in teleost fishes. |
| topic | Animals Connexins Cell Communication Zebrafish Pigmentation Gap Junctions Gap Junction alpha-5 Protein Fishes Mutation, Missense Fish Proteins Genome-Wide Association Study |
| url | https://pubmed.ncbi.nlm.nih.gov/41708610/ |