Salvato in:
| Autori principali: | , , , , , , , , , , , , , , |
|---|---|
| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Microbial genomics
2026
|
| Soggetti: | |
| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/41712274/ |
| Tags: |
Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne!!
|
| _version_ | 1868266082239774721 |
|---|---|
| author | Winkler, Mia A Hetland, Marit A K Kaspersen, Håkon Pedersen Bakksjø, Ragna-Johanne Bernhoff, Eva Fostervold, Aasmund Hawkey, Jane Lunestad, Bjørn-Tore Marathe, Nachiket P Raffelsberger, Niclas Samuelsen, Ørjan Sunde, Marianne Sundsfjord, Arnfinn Lam, Margaret M C Löhr, Iren H |
| author_facet | Winkler, Mia A Hetland, Marit A K Kaspersen, Håkon Pedersen Bakksjø, Ragna-Johanne Bernhoff, Eva Fostervold, Aasmund Hawkey, Jane Lunestad, Bjørn-Tore Marathe, Nachiket P Raffelsberger, Niclas Samuelsen, Ørjan Sunde, Marianne Sundsfjord, Arnfinn Lam, Margaret M C Löhr, Iren H Winkler, Mia A Hetland, Marit A K Kaspersen, Håkon Pedersen Bakksjø, Ragna-Johanne Bernhoff, Eva Fostervold, Aasmund Hawkey, Jane Lunestad, Bjørn-Tore Marathe, Nachiket P Raffelsberger, Niclas Samuelsen, Ørjan Sunde, Marianne Sundsfjord, Arnfinn Lam, Margaret M C Löhr, Iren H |
| collection | PubMed - marine biology |
| contents | A One Health study of species complex plasmids shows a highly diverse and ecologically adaptable plasmidome. Winkler, Mia A Hetland, Marit A K Kaspersen, Håkon Pedersen Bakksjø, Ragna-Johanne Bernhoff, Eva Fostervold, Aasmund Hawkey, Jane Lunestad, Bjørn-Tore Marathe, Nachiket P Raffelsberger, Niclas Samuelsen, Ørjan Sunde, Marianne Sundsfjord, Arnfinn Lam, Margaret M C Löhr, Iren H Klebsiella pneumoniae Plasmids Humans Animals Klebsiella Infections Gene Transfer, Horizontal Whole Genome Sequencing Virulence Genome, Bacterial Genetic Variation Drug Resistance, Bacterial Norway Phylogeny Extrachromosomal DNA Plasmids play a pivotal role in the horizontal gene transfer (HGT) of antimicrobial resistance (AMR) and virulence determinants among bacteria. Members of the species complex (KpSC) can colonize humans, animals and various environments and frequently cause nosocomial and community-acquired infections in humans. While plasmid-borne AMR genes are prevalent in clinical strains, the diversity, distribution and association of plasmids encoding AMR and virulence across ecological niches remain poorly characterized. Understanding the traits governing successful plasmid transmission within and between ecological niches is critical for developing effective AMR prevention strategies. Here, we identify ecological and structural factors shaping plasmid persistence and dissemination. We analysed the plasmidome (i.e. total genetic content attributable to plasmids) of 578 whole-genome sequenced KpSC isolates collected in Norway between 2001 and 2020 from human (=453), animal (=102) and marine (=23) sources. Plasmids from complete hybrid assemblies were annotated and clustered to evaluate the plasmid diversity and content across niches. Additionally, the representativeness of this plasmid collection was determined by clustering with a global collection of 8,656 circularized KpSC plasmids. In total, 1,415 circularized plasmids were identified and grouped according to rearrangement distance using Pling, resulting in 130 clusters (≥2 plasmids each), of which 36% (=47) contained plasmids from more than one niche. The plasmids exhibited significant diversity, as 37% (=524) remained singletons after clustering. AMR and virulence genes existed across diverse clusters and singletons but predominantly resided on 120-250 kbp conjugative or mobilizable plasmids harbouring various transposable elements. Human isolates carried higher overall plasmid burdens and harboured most AMR-encoding plasmids, while animal isolates were significantly enriched for virulence plasmids ( |
| format | Artículo científico |
| id | pubmed_41712274 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Microbial genomics |
| record_format | pubmed |
| spellingShingle | A One Health study of species complex plasmids shows a highly diverse and ecologically adaptable plasmidome. Winkler, Mia A Hetland, Marit A K Kaspersen, Håkon Pedersen Bakksjø, Ragna-Johanne Bernhoff, Eva Fostervold, Aasmund Hawkey, Jane Lunestad, Bjørn-Tore Marathe, Nachiket P Raffelsberger, Niclas Samuelsen, Ørjan Sunde, Marianne Sundsfjord, Arnfinn Lam, Margaret M C Löhr, Iren H Klebsiella pneumoniae Plasmids Humans Animals Klebsiella Infections Gene Transfer, Horizontal Whole Genome Sequencing Virulence Genome, Bacterial Genetic Variation Drug Resistance, Bacterial Norway Phylogeny Extrachromosomal DNA A One Health study of species complex plasmids shows a highly diverse and ecologically adaptable plasmidome. Winkler, Mia A Hetland, Marit A K Kaspersen, Håkon Pedersen Bakksjø, Ragna-Johanne Bernhoff, Eva Fostervold, Aasmund Hawkey, Jane Lunestad, Bjørn-Tore Marathe, Nachiket P Raffelsberger, Niclas Samuelsen, Ørjan Sunde, Marianne Sundsfjord, Arnfinn Lam, Margaret M C Löhr, Iren H Klebsiella pneumoniae Plasmids Humans Animals Klebsiella Infections Gene Transfer, Horizontal Whole Genome Sequencing Virulence Genome, Bacterial Genetic Variation Drug Resistance, Bacterial Norway Phylogeny Extrachromosomal DNA Plasmids play a pivotal role in the horizontal gene transfer (HGT) of antimicrobial resistance (AMR) and virulence determinants among bacteria. Members of the species complex (KpSC) can colonize humans, animals and various environments and frequently cause nosocomial and community-acquired infections in humans. While plasmid-borne AMR genes are prevalent in clinical strains, the diversity, distribution and association of plasmids encoding AMR and virulence across ecological niches remain poorly characterized. Understanding the traits governing successful plasmid transmission within and between ecological niches is critical for developing effective AMR prevention strategies. Here, we identify ecological and structural factors shaping plasmid persistence and dissemination. We analysed the plasmidome (i.e. total genetic content attributable to plasmids) of 578 whole-genome sequenced KpSC isolates collected in Norway between 2001 and 2020 from human (=453), animal (=102) and marine (=23) sources. Plasmids from complete hybrid assemblies were annotated and clustered to evaluate the plasmid diversity and content across niches. Additionally, the representativeness of this plasmid collection was determined by clustering with a global collection of 8,656 circularized KpSC plasmids. In total, 1,415 circularized plasmids were identified and grouped according to rearrangement distance using Pling, resulting in 130 clusters (≥2 plasmids each), of which 36% (=47) contained plasmids from more than one niche. The plasmids exhibited significant diversity, as 37% (=524) remained singletons after clustering. AMR and virulence genes existed across diverse clusters and singletons but predominantly resided on 120-250 kbp conjugative or mobilizable plasmids harbouring various transposable elements. Human isolates carried higher overall plasmid burdens and harboured most AMR-encoding plasmids, while animal isolates were significantly enriched for virulence plasmids ( |
| title | A One Health study of species complex plasmids shows a highly diverse and ecologically adaptable plasmidome. |
| topic | Klebsiella pneumoniae Plasmids Humans Animals Klebsiella Infections Gene Transfer, Horizontal Whole Genome Sequencing Virulence Genome, Bacterial Genetic Variation Drug Resistance, Bacterial Norway Phylogeny Extrachromosomal DNA |
| url | https://pubmed.ncbi.nlm.nih.gov/41712274/ |